Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic
It was recognized only recently that subglacial ecosystems support considerable methanogenic activity, thus significantly contributing the global methane production. However, only limited knowledge is available on the physiological characteristics of this kind of methanogenic community because of th...
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ftdoajarticles:oai:doaj.org/article:77b155407ee24291b9330ad083b5eee6 2023-05-15T13:37:57+02:00 Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic Hongmei Ma Wenkai Yan Xiang Xiao Guitao Shi Yuansheng Li Bo Sun Yinke Dou Yu Zhang 2018-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2018.00237 https://doaj.org/article/77b155407ee24291b9330ad083b5eee6 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fmicb.2018.00237/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2018.00237 https://doaj.org/article/77b155407ee24291b9330ad083b5eee6 Frontiers in Microbiology, Vol 9 (2018) hydrogenotrophic methanogenesis East Antarctic subglacial ecosystem mcrA gene ex situ cultivation climate change Microbiology QR1-502 article 2018 ftdoajarticles https://doi.org/10.3389/fmicb.2018.00237 2022-12-31T06:14:51Z It was recognized only recently that subglacial ecosystems support considerable methanogenic activity, thus significantly contributing the global methane production. However, only limited knowledge is available on the physiological characteristics of this kind of methanogenic community because of the technical constraints associated with sampling and cultivation under corresponding environmental conditions. To elucidate methanogenesis beneath the glacial margin in East Antarctic Ice Sheet, we took an integrated approach that included cultivation of microbes associated with the sediment samples in the lab and analysis of mcrA gene therein. After 7 months of incubation, the highest rate of methanogenesis [398 (pmol/day)/gram] was observed at 1°C on a supply of H2. The rates of methanogenesis were lower on acetate or unamended substrate than on H2. The rates on these two substrates increased when the temperature was raised. Methanomicrobiales predominated before and after prolonged incubation, regardless whether H2, acetate, or unamended substrate were the energy source. Therefore, it was inferred that psychrophilic hydrogenotrophic methanogenesis was the primary methane-producing pathway in the subglacial ecosystem we sampled. These findings highlight the effects of temperature and substrate on potential methanogenesis in the subglacial sediment of this area, and may help us for a better estimation on the Antarctica methane production in a changing climate. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ice Sheet Directory of Open Access Journals: DOAJ Articles Antarctic East Antarctic Ice Sheet Larsemann Hills ENVELOPE(76.217,76.217,-69.400,-69.400) Frontiers in Microbiology 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
hydrogenotrophic methanogenesis East Antarctic subglacial ecosystem mcrA gene ex situ cultivation climate change Microbiology QR1-502 |
spellingShingle |
hydrogenotrophic methanogenesis East Antarctic subglacial ecosystem mcrA gene ex situ cultivation climate change Microbiology QR1-502 Hongmei Ma Wenkai Yan Xiang Xiao Guitao Shi Yuansheng Li Bo Sun Yinke Dou Yu Zhang Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic |
topic_facet |
hydrogenotrophic methanogenesis East Antarctic subglacial ecosystem mcrA gene ex situ cultivation climate change Microbiology QR1-502 |
description |
It was recognized only recently that subglacial ecosystems support considerable methanogenic activity, thus significantly contributing the global methane production. However, only limited knowledge is available on the physiological characteristics of this kind of methanogenic community because of the technical constraints associated with sampling and cultivation under corresponding environmental conditions. To elucidate methanogenesis beneath the glacial margin in East Antarctic Ice Sheet, we took an integrated approach that included cultivation of microbes associated with the sediment samples in the lab and analysis of mcrA gene therein. After 7 months of incubation, the highest rate of methanogenesis [398 (pmol/day)/gram] was observed at 1°C on a supply of H2. The rates of methanogenesis were lower on acetate or unamended substrate than on H2. The rates on these two substrates increased when the temperature was raised. Methanomicrobiales predominated before and after prolonged incubation, regardless whether H2, acetate, or unamended substrate were the energy source. Therefore, it was inferred that psychrophilic hydrogenotrophic methanogenesis was the primary methane-producing pathway in the subglacial ecosystem we sampled. These findings highlight the effects of temperature and substrate on potential methanogenesis in the subglacial sediment of this area, and may help us for a better estimation on the Antarctica methane production in a changing climate. |
format |
Article in Journal/Newspaper |
author |
Hongmei Ma Wenkai Yan Xiang Xiao Guitao Shi Yuansheng Li Bo Sun Yinke Dou Yu Zhang |
author_facet |
Hongmei Ma Wenkai Yan Xiang Xiao Guitao Shi Yuansheng Li Bo Sun Yinke Dou Yu Zhang |
author_sort |
Hongmei Ma |
title |
Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic |
title_short |
Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic |
title_full |
Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic |
title_fullStr |
Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic |
title_full_unstemmed |
Ex Situ Culturing Experiments Revealed Psychrophilic Hydrogentrophic Methanogenesis Being the Potential Dominant Methane-Producing Pathway in Subglacial Sediment in Larsemann Hills, Antarctic |
title_sort |
ex situ culturing experiments revealed psychrophilic hydrogentrophic methanogenesis being the potential dominant methane-producing pathway in subglacial sediment in larsemann hills, antarctic |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.00237 https://doaj.org/article/77b155407ee24291b9330ad083b5eee6 |
long_lat |
ENVELOPE(76.217,76.217,-69.400,-69.400) |
geographic |
Antarctic East Antarctic Ice Sheet Larsemann Hills |
geographic_facet |
Antarctic East Antarctic Ice Sheet Larsemann Hills |
genre |
Antarc* Antarctic Antarctica Ice Sheet |
genre_facet |
Antarc* Antarctic Antarctica Ice Sheet |
op_source |
Frontiers in Microbiology, Vol 9 (2018) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00237/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2018.00237 https://doaj.org/article/77b155407ee24291b9330ad083b5eee6 |
op_doi |
https://doi.org/10.3389/fmicb.2018.00237 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
_version_ |
1766099961008619520 |