Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens Teste dot-ELISA para detecção de anticorpos anti-Cysticercus cellulosae em líquido cefalorraquiano utilizando um novo suporte (tecido de poliéster-resina) e antígenos de Cysticercus longicollis

A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) cova...

Full description

Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Adelaide José Vaz, Paulo Mutuko Nakamura, Mario E. Camargo, Eide Dias Camargo, A. Walter Ferreira
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1996
Subjects:
Neurocysticercosis
Dot-ELISA
Cerebrospinal fluid
Cysticercus cellulosae
Cysticercus longicollis
Resin-treated polyester fabric
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651996000600001
https://doaj.org/article/7772856640604cfcb3011489e5da6de5
Description
Summary:A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) covalently bound to a new solid phase consisting of polyester fabric treated with N-methylol-acrylamide resin (dot-RT). The test was performed at room temperature, with reduced incubation times and with no need for special care in the manipulation of the support. The sensitivity rates obtained were 95.1% for antigen Cc and 97.6% for antigen Cl. Specificity was 90.6% when Cc was used, and 96.9% and 100% when M-Cl and VF-Cl were used, respectively. No significant differences in titer were observed between tests carried out with homologous and heterologous antigens. The low cost and easy execution of the dot-RT test using antigen extracts of Cysticercus longicollis indicate the test for use in the immunodiagnosis of human neurocysticercosis. Foi desenvolvido o teste dot-ELISA para detecção de anticorpos em líquido cefalorraquiano (LCR) no diagnóstico imunológico da neurocisticercose humana, utilizando antígenos de membrana e escólex de Cysticercus cellulosae (M+E-Cc) e, alternativamente, membrana (M) e líquido vesicular (LV) de Cysticercus longicollis (Cl) covalentemente ligados a um novo suporte constituído de tecido de poliéster-resina de N-metilol-acrilamida (dot-TR). O teste foi realizado à temperatura ambiente, com tempos de incubação reduzidos e sem necessidade de cuidados na manipulação do suporte. A sensibilidade obtida foi de 95,1% para o antígeno Cc e 97,6% para o Cl. A especificidade foi de 90,6% quando o antígeno Cc foi usado, e 96,9% e 100% para M-Cl e LV-Cl, respectivamente. Não foi observada diferença significativa entre os antígenos homólogo e heterólogo. O baixo custo e a fácil execução do teste dot-TR empregando extratos antigênicos de Cysticercus longicollis indicam que pode ser empregado como alternativa ...

Similar Items