DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes
Notacanthiformes Goodrich, 1909 is an order of deep-sea, benthopelagic or benthic fishes distributed from the continental slope to the abyssal plain, at a depth of between 125 and 4,900 m, but mostly occurring at depths of 450-2,500 m. They are characterized by an eel-like body, a snout projecting c...
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ftdoajarticles:oai:doaj.org/article:774a0a3887074f5c8228e5770c907c85 2023-05-15T17:30:08+02:00 DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes David Barros-García Juan Carlos Arronte Lourdes Fernandez 2015-11-01T00:00:00Z https://doi.org/10.3389/conf.FMARS.2015.03.00179 https://doaj.org/article/774a0a3887074f5c8228e5770c907c85 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/conf.FMARS.2015.03.00179/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/conf.FMARS.2015.03.00179 https://doaj.org/article/774a0a3887074f5c8228e5770c907c85 Frontiers in Marine Science, Vol 2 (2015) Population Genetics fish DNA barcoding systematics Evolution Molecular Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2015 ftdoajarticles https://doi.org/10.3389/conf.FMARS.2015.03.00179 2022-12-31T14:01:17Z Notacanthiformes Goodrich, 1909 is an order of deep-sea, benthopelagic or benthic fishes distributed from the continental slope to the abyssal plain, at a depth of between 125 and 4,900 m, but mostly occurring at depths of 450-2,500 m. They are characterized by an eel-like body, a snout projecting conspicuously beyond the mouth, large connective tissue nodules inserted between the pterygoid arch and maxilla and pelvic fin webs joined in the ventral midline. Fishes from this order were classified applying DNA barcoding. Cytochrome c oxidase subunit I (COI) sequences belonging to new North Atlantic specimens and already deposited BOLD public records were used. The specimens from the two families of the order, Halosauridae (halosaurs) and Notacanthidae (spiny eels), formed separated monophyletic clades in neighbor-joining trees and the sequences clustered as coherent species. Nine out of 16 species of Halosauridae and 9 out of 10 species of Notacanthidae were represented including 166 sequences of which 96% were successfully identified. The DNA barcode of the rare species Lipogenys gillii was obtained for the first time ever. The DNA barcode was further tested by exploring the genetic structure and historical demography of four species of notacanthiforms from five sample locations of the North Atlantic and South West Pacific. Neutrality tests, mismatch distribution and haplotype networks analyses pointed to a past bottleneck episode followed by a fast demographic expansion for all the samples. The genetic structure of the abyssal halosaur Halosauropsis macrochir showed no significant differences between the North Atlantic and South West Pacific samples. DNA barcoding was successful in validating field identifications and assigning species names to sequences of notacanthiforms worldwide. These results constitute a first example of high connectivity and gene flow in this group of deep-sea fish species. The historical demography suggests population variations that may be due to past glaciation events. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Marine Science 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Population Genetics fish DNA barcoding systematics Evolution Molecular Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Population Genetics fish DNA barcoding systematics Evolution Molecular Science Q General. Including nature conservation geographical distribution QH1-199.5 David Barros-García Juan Carlos Arronte Lourdes Fernandez DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes |
topic_facet |
Population Genetics fish DNA barcoding systematics Evolution Molecular Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Notacanthiformes Goodrich, 1909 is an order of deep-sea, benthopelagic or benthic fishes distributed from the continental slope to the abyssal plain, at a depth of between 125 and 4,900 m, but mostly occurring at depths of 450-2,500 m. They are characterized by an eel-like body, a snout projecting conspicuously beyond the mouth, large connective tissue nodules inserted between the pterygoid arch and maxilla and pelvic fin webs joined in the ventral midline. Fishes from this order were classified applying DNA barcoding. Cytochrome c oxidase subunit I (COI) sequences belonging to new North Atlantic specimens and already deposited BOLD public records were used. The specimens from the two families of the order, Halosauridae (halosaurs) and Notacanthidae (spiny eels), formed separated monophyletic clades in neighbor-joining trees and the sequences clustered as coherent species. Nine out of 16 species of Halosauridae and 9 out of 10 species of Notacanthidae were represented including 166 sequences of which 96% were successfully identified. The DNA barcode of the rare species Lipogenys gillii was obtained for the first time ever. The DNA barcode was further tested by exploring the genetic structure and historical demography of four species of notacanthiforms from five sample locations of the North Atlantic and South West Pacific. Neutrality tests, mismatch distribution and haplotype networks analyses pointed to a past bottleneck episode followed by a fast demographic expansion for all the samples. The genetic structure of the abyssal halosaur Halosauropsis macrochir showed no significant differences between the North Atlantic and South West Pacific samples. DNA barcoding was successful in validating field identifications and assigning species names to sequences of notacanthiforms worldwide. These results constitute a first example of high connectivity and gene flow in this group of deep-sea fish species. The historical demography suggests population variations that may be due to past glaciation events. |
format |
Article in Journal/Newspaper |
author |
David Barros-García Juan Carlos Arronte Lourdes Fernandez |
author_facet |
David Barros-García Juan Carlos Arronte Lourdes Fernandez |
author_sort |
David Barros-García |
title |
DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes |
title_short |
DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes |
title_full |
DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes |
title_fullStr |
DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes |
title_full_unstemmed |
DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes |
title_sort |
dna barcoding and genetic structure analysis of deep-sea notacanthiform fishes |
publisher |
Frontiers Media S.A. |
publishDate |
2015 |
url |
https://doi.org/10.3389/conf.FMARS.2015.03.00179 https://doaj.org/article/774a0a3887074f5c8228e5770c907c85 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Frontiers in Marine Science, Vol 2 (2015) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/conf.FMARS.2015.03.00179/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/conf.FMARS.2015.03.00179 https://doaj.org/article/774a0a3887074f5c8228e5770c907c85 |
op_doi |
https://doi.org/10.3389/conf.FMARS.2015.03.00179 |
container_title |
Frontiers in Marine Science |
container_volume |
2 |
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1766125931699634176 |