Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo)
Abstract Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Portfolio
2024
|
Subjects: | |
Online Access: | https://doi.org/10.1038/s41598-024-52975-8 https://doaj.org/article/770d2e6669fc4ff3a4a135bbe00bd1bf |
id |
ftdoajarticles:oai:doaj.org/article:770d2e6669fc4ff3a4a135bbe00bd1bf |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:770d2e6669fc4ff3a4a135bbe00bd1bf 2024-09-15T18:26:08+00:00 Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) Niki Chondrelli Emily Kuehn Sara Meurling Maria Cortázar-Chinarro Anssi Laurila Jacob Höglund 2024-01-01T00:00:00Z https://doi.org/10.1038/s41598-024-52975-8 https://doaj.org/article/770d2e6669fc4ff3a4a135bbe00bd1bf EN eng Nature Portfolio https://doi.org/10.1038/s41598-024-52975-8 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-024-52975-8 2045-2322 https://doaj.org/article/770d2e6669fc4ff3a4a135bbe00bd1bf Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024) Medicine R Science Q article 2024 ftdoajarticles https://doi.org/10.1038/s41598-024-52975-8 2024-08-05T17:49:52Z Abstract Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected B. bufo eggs in southern and northern Sweden, and infected the laboratory-raised metamorphs with two strains of the global panzoonotic lineage Bd-GPL. Differential expression analysis showed significant differences between infected and control individuals in both liver and skin. The skin samples showed no discernible differences in gene expression between the two strains used, while liver samples were differentiated by strain, with one of the strains eliciting no immune response from infected toads. Immune system genes were overexpressed in skin samples from surviving infected individuals, while in liver samples the pattern was more diffuse. Splitting samples by population revealed a stronger immune response in northern individuals. Differences in transcriptional regulation between populations are particularly relevant to study in Swedish amphibians, which may have experienced varying exposure to Bd. Earlier exposure to this pathogen and subsequent adaptation or selection pressure may contribute to the survival of some populations over others, while standing genetic diversity in different populations may also affect the infection outcome. Article in Journal/Newspaper Northern Sweden Directory of Open Access Journals: DOAJ Articles Scientific Reports 14 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Niki Chondrelli Emily Kuehn Sara Meurling Maria Cortázar-Chinarro Anssi Laurila Jacob Höglund Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
topic_facet |
Medicine R Science Q |
description |
Abstract Batrachochytrium dendrobatidis (Bd) is a fungal pathogen that has decimated amphibian populations worldwide for several decades. We examined the changes in gene expression in response to Bd infection in two populations of the common toad, Bufo bufo, in a laboratory experiment. We collected B. bufo eggs in southern and northern Sweden, and infected the laboratory-raised metamorphs with two strains of the global panzoonotic lineage Bd-GPL. Differential expression analysis showed significant differences between infected and control individuals in both liver and skin. The skin samples showed no discernible differences in gene expression between the two strains used, while liver samples were differentiated by strain, with one of the strains eliciting no immune response from infected toads. Immune system genes were overexpressed in skin samples from surviving infected individuals, while in liver samples the pattern was more diffuse. Splitting samples by population revealed a stronger immune response in northern individuals. Differences in transcriptional regulation between populations are particularly relevant to study in Swedish amphibians, which may have experienced varying exposure to Bd. Earlier exposure to this pathogen and subsequent adaptation or selection pressure may contribute to the survival of some populations over others, while standing genetic diversity in different populations may also affect the infection outcome. |
format |
Article in Journal/Newspaper |
author |
Niki Chondrelli Emily Kuehn Sara Meurling Maria Cortázar-Chinarro Anssi Laurila Jacob Höglund |
author_facet |
Niki Chondrelli Emily Kuehn Sara Meurling Maria Cortázar-Chinarro Anssi Laurila Jacob Höglund |
author_sort |
Niki Chondrelli |
title |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_short |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_full |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_fullStr |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_full_unstemmed |
Batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (Bufo bufo) |
title_sort |
batrachochytrium dendrobatidis strain affects transcriptomic response in liver but not skin in latitudinal populations of the common toad (bufo bufo) |
publisher |
Nature Portfolio |
publishDate |
2024 |
url |
https://doi.org/10.1038/s41598-024-52975-8 https://doaj.org/article/770d2e6669fc4ff3a4a135bbe00bd1bf |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024) |
op_relation |
https://doi.org/10.1038/s41598-024-52975-8 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-024-52975-8 2045-2322 https://doaj.org/article/770d2e6669fc4ff3a4a135bbe00bd1bf |
op_doi |
https://doi.org/10.1038/s41598-024-52975-8 |
container_title |
Scientific Reports |
container_volume |
14 |
container_issue |
1 |
_version_ |
1810466592978370560 |