Phytochemical profiling of Artocarpus lakoocha Roxb. leaf methanol extract and its antioxidant, antimicrobial and antioxidative activities

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Ekta Bhattacharya, Rajashree Dutta, Swati Chakraborty, Suparna Mandal Biswas
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2019
• Subjects: moraceae; bioactive compounds; natural pharmaceuticals; diethyl phthalate; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic; Lambda
• Online Access: https://doi.org/10.4103/2221-1691.270984; https://doaj.org/article/76cb10d57f4b456aaf4c63eff46aa57f

Objective: To explore the phytochemical profile of Artocarpus lakoocha Roxb. leaves both qualitatively and quantitatively, and validate its role as a potent antioxidant and antimicrobial agent. Methods: Extraction and isolation of different compounds were done from the leaves of Artocarpus lakoocha based on solvent fractionation method. Subsequently, quantitative and qualitative phytochemical profiling along with antioxidant, antimicrobial and antioxidative activities were tested following standard protocols. Results: Among the five fractions, methanol fraction of Artocarpus lakoocha exhibited higher content of phytochemical compounds [phenols = (3 175.21 ±290.43) mg GAE/g dry extract, flavonoids = (1 173.15±47.52) mg QE/g dry extract and tannins = (923.53±95.21) mg TAE/ g dry extract] as compared to other fractions. The methanol fraction showed the highest antioxidant activity in DPPH and ABTS radical scavenging assays with IC50 of (111.98±34.20) μg/mL and (138.26±0.66) μg/mL, respectively, and the best reduction potential with a value of (316.81±2.96) mg QE/g dry extract in reducing power assay. There was significant correlation between the amount of phytochemicals and antioxidant activities. Moreover, the extract successfully protected Lambda phage DNA from damage at 5 and 6 mg/mL concentration and exhibited substantial bactericidal as well as fungicidal activity. The GC-MS analysis of methanol fraction of Artocarpus lakoocha revealed diethyl phthalate as the main phytochemical compound, along with 3,4-dihydroxymandelic acid, 9-octyl eicosane and 7,8-didehydro-3- methoxy-17-methyl-6-methylene morphinan. Conclusions: The methanol fraction of Artocarpus lakoocha could be used as a potent antioxidant and antimicrobial agent for sustainable agriculture and pharmaceutical purposes.