Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste.
The live attenuated tetravalent CYD-TDV vaccine (Dengvaxia) is effective but has scarcely been used due to safety concerns among seronegative recipients. Rapid diagnostic tests (RDTs) which can accurately determine individual dengue serostatus are needed for use in pre-vaccination screening. This st...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0010877 https://doaj.org/article/76c291fffcc942108820b307faf7b902 |
id |
ftdoajarticles:oai:doaj.org/article:76c291fffcc942108820b307faf7b902 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:76c291fffcc942108820b307faf7b902 2023-05-15T15:13:39+02:00 Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. Paul Arkell Maria Tanesi Nelia Gomes Josefina C Joao Tessa Oakley Frederico Bosco Jennifer Yan Nicholas S S Fancourt Joshua R Francis 2022-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010877 https://doaj.org/article/76c291fffcc942108820b307faf7b902 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010877 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010877 https://doaj.org/article/76c291fffcc942108820b307faf7b902 PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010877 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010877 2022-12-30T19:35:03Z The live attenuated tetravalent CYD-TDV vaccine (Dengvaxia) is effective but has scarcely been used due to safety concerns among seronegative recipients. Rapid diagnostic tests (RDTs) which can accurately determine individual dengue serostatus are needed for use in pre-vaccination screening. This study aimed to determine the performance of existing RDTs (which have been designed to detect levels of immunoglobulin G, IgG, associated with acute post-primary dengue) when repurposed for detection of previous dengue infection (where concentrations of IgG are typically lower). A convenience sample of four-hundred-and-six participants including 217 children were recruited during a community serosurvey. Whole blood was collected by phlebotomy and tested using Bioline Dengue IgG/IgM (Abbott) and Standard Q Dengue IgM/IgG (SD Biosensor) RDTs in the field. Serum samples from the same individuals were also tested at National Health Laboratory. The Panbio indirect IgG ELISA was used as a reference test. Reference testing determined that 370 (91.1%) participants were dengue IgG seropositive. Both assays were highly specific (100.0%) but had low sensitivity (Bioline = 21.1% and Standard Q = 4.6%) when used in the field. Sensitivity was improved when RDTs were used under laboratory conditions, and when assays were allowed to run beyond manufacturer recommendations and read at a delayed time-point, but specificity was reduced. Efforts to develop RDTs with high sensitivity and specificity for prior dengue infection which can be operationalised for pre-vaccination screening are ongoing. Performance of forthcoming candidate assays should be tested under field conditions with blood samples, as well as in the laboratory. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Abbott ENVELOPE(-62.133,-62.133,-64.100,-64.100) Arctic PLOS Neglected Tropical Diseases 16 11 e0010877 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Paul Arkell Maria Tanesi Nelia Gomes Josefina C Joao Tessa Oakley Frederico Bosco Jennifer Yan Nicholas S S Fancourt Joshua R Francis Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The live attenuated tetravalent CYD-TDV vaccine (Dengvaxia) is effective but has scarcely been used due to safety concerns among seronegative recipients. Rapid diagnostic tests (RDTs) which can accurately determine individual dengue serostatus are needed for use in pre-vaccination screening. This study aimed to determine the performance of existing RDTs (which have been designed to detect levels of immunoglobulin G, IgG, associated with acute post-primary dengue) when repurposed for detection of previous dengue infection (where concentrations of IgG are typically lower). A convenience sample of four-hundred-and-six participants including 217 children were recruited during a community serosurvey. Whole blood was collected by phlebotomy and tested using Bioline Dengue IgG/IgM (Abbott) and Standard Q Dengue IgM/IgG (SD Biosensor) RDTs in the field. Serum samples from the same individuals were also tested at National Health Laboratory. The Panbio indirect IgG ELISA was used as a reference test. Reference testing determined that 370 (91.1%) participants were dengue IgG seropositive. Both assays were highly specific (100.0%) but had low sensitivity (Bioline = 21.1% and Standard Q = 4.6%) when used in the field. Sensitivity was improved when RDTs were used under laboratory conditions, and when assays were allowed to run beyond manufacturer recommendations and read at a delayed time-point, but specificity was reduced. Efforts to develop RDTs with high sensitivity and specificity for prior dengue infection which can be operationalised for pre-vaccination screening are ongoing. Performance of forthcoming candidate assays should be tested under field conditions with blood samples, as well as in the laboratory. |
format |
Article in Journal/Newspaper |
author |
Paul Arkell Maria Tanesi Nelia Gomes Josefina C Joao Tessa Oakley Frederico Bosco Jennifer Yan Nicholas S S Fancourt Joshua R Francis |
author_facet |
Paul Arkell Maria Tanesi Nelia Gomes Josefina C Joao Tessa Oakley Frederico Bosco Jennifer Yan Nicholas S S Fancourt Joshua R Francis |
author_sort |
Paul Arkell |
title |
Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. |
title_short |
Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. |
title_full |
Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. |
title_fullStr |
Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. |
title_full_unstemmed |
Field evaluation of rapid diagnostic tests to determine dengue serostatus in Timor-Leste. |
title_sort |
field evaluation of rapid diagnostic tests to determine dengue serostatus in timor-leste. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010877 https://doaj.org/article/76c291fffcc942108820b307faf7b902 |
long_lat |
ENVELOPE(-62.133,-62.133,-64.100,-64.100) |
geographic |
Abbott Arctic |
geographic_facet |
Abbott Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010877 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010877 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010877 https://doaj.org/article/76c291fffcc942108820b307faf7b902 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010877 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
11 |
container_start_page |
e0010877 |
_version_ |
1766344179341852672 |