In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations
Trypanosoma cruzi, the etiologic Chagas' disease agent, induces changes in protein pattern of the human placenta syncytiotrophoblast. The glucose transporter protein-1 (GLUT1) is the primary isoform involved in transplacental glucose transport. We carried out in vitro assays to determine if T....
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ftdoajarticles:oai:doaj.org/article:767a1f30d1ff48c8be152db5c0ddf6a8 2023-05-15T15:05:17+02:00 In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations Luciana Mezzano Gastón Repossi Ricardo E. Fretes Susana Lin María José Sartori Sofía G. Parisi de Fabro 2012-01-01T00:00:00Z https://doi.org/10.1155/2012/969243 https://doaj.org/article/767a1f30d1ff48c8be152db5c0ddf6a8 EN eng Hindawi Limited http://dx.doi.org/10.1155/2012/969243 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2012/969243 https://doaj.org/article/767a1f30d1ff48c8be152db5c0ddf6a8 Journal of Tropical Medicine, Vol 2012 (2012) Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1155/2012/969243 2022-12-31T01:34:27Z Trypanosoma cruzi, the etiologic Chagas' disease agent, induces changes in protein pattern of the human placenta syncytiotrophoblast. The glucose transporter protein-1 (GLUT1) is the primary isoform involved in transplacental glucose transport. We carried out in vitro assays to determine if T. cruzi infection would induce changes in placental GLUT1 protein expression under normal and high concentration of glucose. Using Western blot and immunohistological techniques, GLUT1 expression was determined in normal placental villi cultured under normal or high concentrations of glucose, with or without in vitro T. cruzi infection, for 24 and 48 hours. High glucose media or T. cruzi infection alone reduced GLUT1 expression. A yet more accentuated reduction was observed when infection and high glucose condition took place together. We inform, for the first time, that T. cruzi infection may induce reduction of GLUT1 expression under normal and high glucose concentrations, and this effect is synergic to high glucose concentrations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2012 1 8 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Luciana Mezzano Gastón Repossi Ricardo E. Fretes Susana Lin María José Sartori Sofía G. Parisi de Fabro In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Trypanosoma cruzi, the etiologic Chagas' disease agent, induces changes in protein pattern of the human placenta syncytiotrophoblast. The glucose transporter protein-1 (GLUT1) is the primary isoform involved in transplacental glucose transport. We carried out in vitro assays to determine if T. cruzi infection would induce changes in placental GLUT1 protein expression under normal and high concentration of glucose. Using Western blot and immunohistological techniques, GLUT1 expression was determined in normal placental villi cultured under normal or high concentrations of glucose, with or without in vitro T. cruzi infection, for 24 and 48 hours. High glucose media or T. cruzi infection alone reduced GLUT1 expression. A yet more accentuated reduction was observed when infection and high glucose condition took place together. We inform, for the first time, that T. cruzi infection may induce reduction of GLUT1 expression under normal and high glucose concentrations, and this effect is synergic to high glucose concentrations. |
format |
Article in Journal/Newspaper |
author |
Luciana Mezzano Gastón Repossi Ricardo E. Fretes Susana Lin María José Sartori Sofía G. Parisi de Fabro |
author_facet |
Luciana Mezzano Gastón Repossi Ricardo E. Fretes Susana Lin María José Sartori Sofía G. Parisi de Fabro |
author_sort |
Luciana Mezzano |
title |
In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations |
title_short |
In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations |
title_full |
In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations |
title_fullStr |
In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations |
title_full_unstemmed |
In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations |
title_sort |
in vitro infection of trypanosoma cruzi causes decrease in glucose transporter protein-1 (glut1) expression in explants of human placental villi cultured under normal and high glucose concentrations |
publisher |
Hindawi Limited |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/969243 https://doaj.org/article/767a1f30d1ff48c8be152db5c0ddf6a8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2012 (2012) |
op_relation |
http://dx.doi.org/10.1155/2012/969243 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2012/969243 https://doaj.org/article/767a1f30d1ff48c8be152db5c0ddf6a8 |
op_doi |
https://doi.org/10.1155/2012/969243 |
container_title |
Journal of Tropical Medicine |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
8 |
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1766337020426190848 |