Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes )
Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, t...
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ftdoajarticles:oai:doaj.org/article:7665c5056e224732bdb2143231f90062 2023-05-15T15:56:36+02:00 Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab 2020-10-01T00:00:00Z https://doi.org/10.3390/ani10101841 https://doaj.org/article/7665c5056e224732bdb2143231f90062 EN eng MDPI AG https://www.mdpi.com/2076-2615/10/10/1841 https://doaj.org/toc/2076-2615 doi:10.3390/ani10101841 2076-2615 https://doaj.org/article/7665c5056e224732bdb2143231f90062 Animals, Vol 10, Iss 1841, p 1841 (2020) Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy Veterinary medicine SF600-1100 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.3390/ani10101841 2022-12-31T01:00:43Z Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, the phylogenetic relationship among these viruses is poorly resolved, since some of them were known as rodent-related so far. The red fox, the most widespread carnivore worldwide, is a known reservoir of several viruses that transmit from wildlife to humans or domestic animals; they might have a defined role in the circulation of rodent-borne HEV. In this study, we performed a HEV survey by heminested RT-PCR (Reverse Transcription PCR) on red fox fecal samples to investigate the presence of HEV in red foxes living in natural conditions, and to explore the origin of the virus via phylogenetic analysis. Out of the 26 investigated samples, HEV RNA was identified in one sample. Following Sanger sequencing, the novel sequence displayed 91% identity on the nucleotide level with recently published European common vole-HEV derived from Microtus arvalis . In contrast, it shared 85% nucleotide similarity with HEV strains described previously in red foxes. Our results strongly support “the dietary-origin” of unclassified HEV-like strains described from predators that usually prey on rodents. Article in Journal/Newspaper Common vole Microtus arvalis Directory of Open Access Journals: DOAJ Articles Animals 10 10 1841 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy Veterinary medicine SF600-1100 Zoology QL1-991 Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) |
topic_facet |
Hepeviridae Orthohepevirus C cvHEV small mammal zoonoses taxonomy Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Orthohepeviruses (HEV) can infect a wide range of animals, showing a relatively strict host specificity; however, its zoonotic potential, natural transmission in the wildlife are less known. Several new HEV-like viruses have been identified in various animal species, including carnivores; however, the phylogenetic relationship among these viruses is poorly resolved, since some of them were known as rodent-related so far. The red fox, the most widespread carnivore worldwide, is a known reservoir of several viruses that transmit from wildlife to humans or domestic animals; they might have a defined role in the circulation of rodent-borne HEV. In this study, we performed a HEV survey by heminested RT-PCR (Reverse Transcription PCR) on red fox fecal samples to investigate the presence of HEV in red foxes living in natural conditions, and to explore the origin of the virus via phylogenetic analysis. Out of the 26 investigated samples, HEV RNA was identified in one sample. Following Sanger sequencing, the novel sequence displayed 91% identity on the nucleotide level with recently published European common vole-HEV derived from Microtus arvalis . In contrast, it shared 85% nucleotide similarity with HEV strains described previously in red foxes. Our results strongly support “the dietary-origin” of unclassified HEV-like strains described from predators that usually prey on rodents. |
format |
Article in Journal/Newspaper |
author |
Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab |
author_facet |
Zsófia Lanszki Kornélia Kurucz Safia Zeghbib Gábor Kemenesi József Lanszki Ferenc Jakab |
author_sort |
Zsófia Lanszki |
title |
Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) |
title_short |
Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) |
title_full |
Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) |
title_fullStr |
Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) |
title_full_unstemmed |
Identification of Hepatitis E Virus in the Feces of Red Foxes ( Vulpes vulpes ) |
title_sort |
identification of hepatitis e virus in the feces of red foxes ( vulpes vulpes ) |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ani10101841 https://doaj.org/article/7665c5056e224732bdb2143231f90062 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Animals, Vol 10, Iss 1841, p 1841 (2020) |
op_relation |
https://www.mdpi.com/2076-2615/10/10/1841 https://doaj.org/toc/2076-2615 doi:10.3390/ani10101841 2076-2615 https://doaj.org/article/7665c5056e224732bdb2143231f90062 |
op_doi |
https://doi.org/10.3390/ani10101841 |
container_title |
Animals |
container_volume |
10 |
container_issue |
10 |
container_start_page |
1841 |
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1766391997079224320 |