The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313.
Over recent decades, Salmonella infection research has predominantly relied on murine infection models. However, in many cases the infection phenotypes of Salmonella pathovars in mice do not recapitulate human disease. For example, Salmonella Typhimurium ST313 is associated with enhanced invasive in...
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ftdoajarticles:oai:doaj.org/article:7619b0e213f646d79a0796d953ea6446 2023-05-15T15:11:15+02:00 The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. Lizeth Lacharme-Lora Siân V Owen Richard Blundell Rocío Canals Nicolas Wenner Blanca Perez-Sepulveda Wai Yee Fong Rachel Gilroy Paul Wigley Jay C D Hinton 2019-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007540 https://doaj.org/article/7619b0e213f646d79a0796d953ea6446 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007540 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007540 https://doaj.org/article/7619b0e213f646d79a0796d953ea6446 PLoS Neglected Tropical Diseases, Vol 13, Iss 7, p e0007540 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007540 2022-12-31T07:17:08Z Over recent decades, Salmonella infection research has predominantly relied on murine infection models. However, in many cases the infection phenotypes of Salmonella pathovars in mice do not recapitulate human disease. For example, Salmonella Typhimurium ST313 is associated with enhanced invasive infection of immunocompromised people in Africa, but infection of mice and other animal models with ST313 have not consistently reproduced this invasive phenotype. The introduction of alternative infection models could help to improve the quality and reproducibility of pathogenesis research by facilitating larger-scale experiments. To investigate the virulence of S. Typhimurium ST313 in comparison with ST19, a combination of avian and insect disease models were used. We performed experimental infections in five lines of inbred and one line of outbred chickens, as well as in the alternative chick embryo and Galleria mellonella wax moth larvae models. This extensive set of experiments identified broadly similar patterns of disease caused by the African and global pathovariants of Salmonella Typhimurium in the chicken, the chicken embryo and insect models. A comprehensive analysis of all the chicken infection experiments revealed that the African ST313 isolate D23580 had a subtle phenotype of reduced levels of organ colonisation in inbred chickens, relative to ST19 strain 4/74. ST313 isolate D23580 also caused reduced mortality in chicken embryos and insect larvae, when compared with ST19 4/74. We conclude that these three infection models do not reproduce the characteristics of the systemic disease caused by S. Typhimurium ST313 in humans. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 7 e0007540 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lizeth Lacharme-Lora Siân V Owen Richard Blundell Rocío Canals Nicolas Wenner Blanca Perez-Sepulveda Wai Yee Fong Rachel Gilroy Paul Wigley Jay C D Hinton The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Over recent decades, Salmonella infection research has predominantly relied on murine infection models. However, in many cases the infection phenotypes of Salmonella pathovars in mice do not recapitulate human disease. For example, Salmonella Typhimurium ST313 is associated with enhanced invasive infection of immunocompromised people in Africa, but infection of mice and other animal models with ST313 have not consistently reproduced this invasive phenotype. The introduction of alternative infection models could help to improve the quality and reproducibility of pathogenesis research by facilitating larger-scale experiments. To investigate the virulence of S. Typhimurium ST313 in comparison with ST19, a combination of avian and insect disease models were used. We performed experimental infections in five lines of inbred and one line of outbred chickens, as well as in the alternative chick embryo and Galleria mellonella wax moth larvae models. This extensive set of experiments identified broadly similar patterns of disease caused by the African and global pathovariants of Salmonella Typhimurium in the chicken, the chicken embryo and insect models. A comprehensive analysis of all the chicken infection experiments revealed that the African ST313 isolate D23580 had a subtle phenotype of reduced levels of organ colonisation in inbred chickens, relative to ST19 strain 4/74. ST313 isolate D23580 also caused reduced mortality in chicken embryos and insect larvae, when compared with ST19 4/74. We conclude that these three infection models do not reproduce the characteristics of the systemic disease caused by S. Typhimurium ST313 in humans. |
format |
Article in Journal/Newspaper |
author |
Lizeth Lacharme-Lora Siân V Owen Richard Blundell Rocío Canals Nicolas Wenner Blanca Perez-Sepulveda Wai Yee Fong Rachel Gilroy Paul Wigley Jay C D Hinton |
author_facet |
Lizeth Lacharme-Lora Siân V Owen Richard Blundell Rocío Canals Nicolas Wenner Blanca Perez-Sepulveda Wai Yee Fong Rachel Gilroy Paul Wigley Jay C D Hinton |
author_sort |
Lizeth Lacharme-Lora |
title |
The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. |
title_short |
The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. |
title_full |
The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. |
title_fullStr |
The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. |
title_full_unstemmed |
The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313. |
title_sort |
use of chicken and insect infection models to assess the virulence of african salmonella typhimurium st313. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007540 https://doaj.org/article/7619b0e213f646d79a0796d953ea6446 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 7, p e0007540 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007540 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007540 https://doaj.org/article/7619b0e213f646d79a0796d953ea6446 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007540 |
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PLOS Neglected Tropical Diseases |
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13 |
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7 |
container_start_page |
e0007540 |
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1766342139941224448 |