Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species
Low-oxygen stress, mainly caused by soil flooding, is a serious abiotic stress affecting crop productivity worldwide. To understand the mechanisms of low-oxygen stress responses and adaptation of plants, we characterized and compared low-oxygen responses in six species with different accessions of t...
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ftdoajarticles:oai:doaj.org/article:760b69bfa87c4c369298144b0b5632d2 2023-05-15T18:07:22+02:00 Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species Ji-Hye Hwang Si-in Yu Byeong-ha Lee Dong-Hee Lee 2020-03-01T00:00:00Z https://doi.org/10.3390/ijms21051787 https://doaj.org/article/760b69bfa87c4c369298144b0b5632d2 EN eng MDPI AG https://www.mdpi.com/1422-0067/21/5/1787 https://doaj.org/toc/1422-0067 1422-0067 doi:10.3390/ijms21051787 https://doaj.org/article/760b69bfa87c4c369298144b0b5632d2 International Journal of Molecular Sciences, Vol 21, Iss 5, p 1787 (2020) low oxygen hypoxia energy metabolism brassicaceae gene set enrichment analysis (gsea) Biology (General) QH301-705.5 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/ijms21051787 2022-12-31T11:44:07Z Low-oxygen stress, mainly caused by soil flooding, is a serious abiotic stress affecting crop productivity worldwide. To understand the mechanisms of low-oxygen stress responses and adaptation of plants, we characterized and compared low-oxygen responses in six species with different accessions of the Brassicaceae family. Based on the growth and survival responses to submergence or low-oxygen condition, these accessions could be divided into three groups: (i) Highly tolerant species ( Rorippa islandica and Arabis stelleri ); (ii) moderately tolerant species ( Arabidopsis thaliana [ esk-1 , Ler, Ws and Col-0 ecotype]); and (iii) intolerant species ( Thlaspi arvense , Thellungiella salsuginea [Shandong and Yukon ecotype], and Thellungiella parvula ). Gene expression profiling using Operon Arabidopsis microarray was carried out with RNA from roots of A. thaliana (Col-0), A. stelleri , R. islandica , and T. salsuginea (Shandong) treated with low-oxygen stress (0.1% O 2 /99.9% N 2 ) for 0, 1, 3, 8, 24, and 72 h. We performed a comparative analysis of the gene expression profiles using the gene set enrichment analysis (GSEA) method. Our comparative analysis suggested that under low-oxygen stress each species distinctively reconfigures the energy metabolic pathways including sucrose−starch metabolism, glycolysis, fermentation and nitrogen metabolism, tricarboxylic acid flow, and fatty acid degradation via beta oxidation and glyoxylate cycle. In A. thaliana , a moderately tolerant species, the dynamical reconfiguration of energy metabolisms occurred in the early time points of low-oxygen treatment, but the energy reconfiguration in the late time points was not as dynamic as in the early time points. Highly tolerant A. stelleri appeared to have high photosynthesis capacity that could produce more O 2 and in turn additional ATP energy to cope with energy depletion caused by low-oxygen stress. R. islandica seemed to retain some ATP energy produced by anaerobic energy metabolism during a prolonged period of low-oxygen ... Article in Journal/Newspaper Rorippa islandica Yukon Directory of Open Access Journals: DOAJ Articles Yukon International Journal of Molecular Sciences 21 5 1787 |
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language |
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topic |
low oxygen hypoxia energy metabolism brassicaceae gene set enrichment analysis (gsea) Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
low oxygen hypoxia energy metabolism brassicaceae gene set enrichment analysis (gsea) Biology (General) QH301-705.5 Chemistry QD1-999 Ji-Hye Hwang Si-in Yu Byeong-ha Lee Dong-Hee Lee Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species |
topic_facet |
low oxygen hypoxia energy metabolism brassicaceae gene set enrichment analysis (gsea) Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Low-oxygen stress, mainly caused by soil flooding, is a serious abiotic stress affecting crop productivity worldwide. To understand the mechanisms of low-oxygen stress responses and adaptation of plants, we characterized and compared low-oxygen responses in six species with different accessions of the Brassicaceae family. Based on the growth and survival responses to submergence or low-oxygen condition, these accessions could be divided into three groups: (i) Highly tolerant species ( Rorippa islandica and Arabis stelleri ); (ii) moderately tolerant species ( Arabidopsis thaliana [ esk-1 , Ler, Ws and Col-0 ecotype]); and (iii) intolerant species ( Thlaspi arvense , Thellungiella salsuginea [Shandong and Yukon ecotype], and Thellungiella parvula ). Gene expression profiling using Operon Arabidopsis microarray was carried out with RNA from roots of A. thaliana (Col-0), A. stelleri , R. islandica , and T. salsuginea (Shandong) treated with low-oxygen stress (0.1% O 2 /99.9% N 2 ) for 0, 1, 3, 8, 24, and 72 h. We performed a comparative analysis of the gene expression profiles using the gene set enrichment analysis (GSEA) method. Our comparative analysis suggested that under low-oxygen stress each species distinctively reconfigures the energy metabolic pathways including sucrose−starch metabolism, glycolysis, fermentation and nitrogen metabolism, tricarboxylic acid flow, and fatty acid degradation via beta oxidation and glyoxylate cycle. In A. thaliana , a moderately tolerant species, the dynamical reconfiguration of energy metabolisms occurred in the early time points of low-oxygen treatment, but the energy reconfiguration in the late time points was not as dynamic as in the early time points. Highly tolerant A. stelleri appeared to have high photosynthesis capacity that could produce more O 2 and in turn additional ATP energy to cope with energy depletion caused by low-oxygen stress. R. islandica seemed to retain some ATP energy produced by anaerobic energy metabolism during a prolonged period of low-oxygen ... |
format |
Article in Journal/Newspaper |
author |
Ji-Hye Hwang Si-in Yu Byeong-ha Lee Dong-Hee Lee |
author_facet |
Ji-Hye Hwang Si-in Yu Byeong-ha Lee Dong-Hee Lee |
author_sort |
Ji-Hye Hwang |
title |
Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species |
title_short |
Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species |
title_full |
Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species |
title_fullStr |
Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species |
title_full_unstemmed |
Modulation of Energy Metabolism Is Important for Low-Oxygen Stress Adaptation in Brassicaceae Species |
title_sort |
modulation of energy metabolism is important for low-oxygen stress adaptation in brassicaceae species |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21051787 https://doaj.org/article/760b69bfa87c4c369298144b0b5632d2 |
geographic |
Yukon |
geographic_facet |
Yukon |
genre |
Rorippa islandica Yukon |
genre_facet |
Rorippa islandica Yukon |
op_source |
International Journal of Molecular Sciences, Vol 21, Iss 5, p 1787 (2020) |
op_relation |
https://www.mdpi.com/1422-0067/21/5/1787 https://doaj.org/toc/1422-0067 1422-0067 doi:10.3390/ijms21051787 https://doaj.org/article/760b69bfa87c4c369298144b0b5632d2 |
op_doi |
https://doi.org/10.3390/ijms21051787 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
5 |
container_start_page |
1787 |
_version_ |
1766179462475415552 |