Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome
ABSTRACT Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mo...
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American Society for Microbiology
2022
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ftdoajarticles:oai:doaj.org/article:75fa1e05d8d14784802567508ecfa8c8 2023-05-15T15:31:15+02:00 Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome Raminta Kazlauskaite Bachar Cheaib Joseph Humble Chloe Heys Umer Zeeshan Ijaz Stephanie Connelly William T. Sloan Julie Russell Laura Martinez-Rubio John Sweetman Alex Kitts Philip McGinnity Philip Lyons Martin S. Llewellyn 2022-06-01T00:00:00Z https://doi.org/10.1128/spectrum.01953-21 https://doaj.org/article/75fa1e05d8d14784802567508ecfa8c8 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/spectrum.01953-21 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.01953-21 2165-0497 https://doaj.org/article/75fa1e05d8d14784802567508ecfa8c8 Microbiology Spectrum, Vol 10, Iss 3 (2022) MOS Atlantic salmon microbiome in vitro gut model NGS Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.1128/spectrum.01953-21 2022-12-30T21:32:25Z ABSTRACT Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators. IMPORTANCE In this paper we report the results of the impact of a ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Microbiology Spectrum 10 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
MOS Atlantic salmon microbiome in vitro gut model NGS Microbiology QR1-502 |
spellingShingle |
MOS Atlantic salmon microbiome in vitro gut model NGS Microbiology QR1-502 Raminta Kazlauskaite Bachar Cheaib Joseph Humble Chloe Heys Umer Zeeshan Ijaz Stephanie Connelly William T. Sloan Julie Russell Laura Martinez-Rubio John Sweetman Alex Kitts Philip McGinnity Philip Lyons Martin S. Llewellyn Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
topic_facet |
MOS Atlantic salmon microbiome in vitro gut model NGS Microbiology QR1-502 |
description |
ABSTRACT Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators. IMPORTANCE In this paper we report the results of the impact of a ... |
format |
Article in Journal/Newspaper |
author |
Raminta Kazlauskaite Bachar Cheaib Joseph Humble Chloe Heys Umer Zeeshan Ijaz Stephanie Connelly William T. Sloan Julie Russell Laura Martinez-Rubio John Sweetman Alex Kitts Philip McGinnity Philip Lyons Martin S. Llewellyn |
author_facet |
Raminta Kazlauskaite Bachar Cheaib Joseph Humble Chloe Heys Umer Zeeshan Ijaz Stephanie Connelly William T. Sloan Julie Russell Laura Martinez-Rubio John Sweetman Alex Kitts Philip McGinnity Philip Lyons Martin S. Llewellyn |
author_sort |
Raminta Kazlauskaite |
title |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_short |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_full |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_fullStr |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_full_unstemmed |
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome |
title_sort |
deploying an in vitro gut model to assay the impact of the mannan-oligosaccharide prebiotic bio-mos on the atlantic salmon (salmo salar) gut microbiome |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
https://doi.org/10.1128/spectrum.01953-21 https://doaj.org/article/75fa1e05d8d14784802567508ecfa8c8 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Microbiology Spectrum, Vol 10, Iss 3 (2022) |
op_relation |
https://journals.asm.org/doi/10.1128/spectrum.01953-21 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.01953-21 2165-0497 https://doaj.org/article/75fa1e05d8d14784802567508ecfa8c8 |
op_doi |
https://doi.org/10.1128/spectrum.01953-21 |
container_title |
Microbiology Spectrum |
container_volume |
10 |
container_issue |
3 |
_version_ |
1766361745181376512 |