A novel rapid test for detecting antibody responses to Loa loa infections.
Ivermectin-based mass drug administration (MDA) programs have achieved remarkable success towards the elimination of onchocerciasis and lymphatic filariasis. However, their full implementation has been hindered in Central Africa by the occurrence of ivermectin-related severe adverse events (SAEs) in...
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ftdoajarticles:oai:doaj.org/article:75e896981e0d41149101d542497e8617 2023-05-15T15:12:44+02:00 A novel rapid test for detecting antibody responses to Loa loa infections. Bijan Pedram Valérie Pasquetto Papa M Drame Yongchang Ji Maria J Gonzalez-Moa Richard K Baldwin Thomas B Nutman Marco A Biamonte 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005741 https://doaj.org/article/75e896981e0d41149101d542497e8617 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5531435?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005741 https://doaj.org/article/75e896981e0d41149101d542497e8617 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005741 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005741 2022-12-31T10:41:52Z Ivermectin-based mass drug administration (MDA) programs have achieved remarkable success towards the elimination of onchocerciasis and lymphatic filariasis. However, their full implementation has been hindered in Central Africa by the occurrence of ivermectin-related severe adverse events (SAEs) in a subset of individuals with high circulating levels of Loa loa microfilariae. Extending MDA to areas with coincident L. loa infection is problematic, and inexpensive point-of-care tests for L. loa are acutely needed. Herein, we present a lateral flow assay (LFA) to identify subjects with a serological response to Ll-SXP-1, a specific and validated marker of L. loa. The test was evaluated on serum samples from patients infected with L. loa (n = 109) and other helminths (n = 204), as well as on uninfected controls (n = 77). When read with the naked eye, the test was 94% sensitive for L. loa infection and was 100% specific when sera from healthy endemic and non-endemic controls or from those with S. stercoralis infections were used as the comparators. When sera of patients with O. volvulus, W. bancrofti, or M. perstans were used as the comparators, the specificity of the LFA was 82%, 87%, and 88%, respectively. A companion smartphone reader allowed measurement of the test line intensities and establishment of cutoff values. With a cutoff of 600 Units, the assay sensitivity decreased to 71%, but the specificity increased to 96% for O. volvulus, 100% for W. bancrofti, and 100% for M. perstans-infected individuals. The LFA may find applications in refining the current maps of L. loa prevalence, which are needed to eliminate onchocerciasis and lymphatic filariasis from the African continent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005741 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bijan Pedram Valérie Pasquetto Papa M Drame Yongchang Ji Maria J Gonzalez-Moa Richard K Baldwin Thomas B Nutman Marco A Biamonte A novel rapid test for detecting antibody responses to Loa loa infections. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Ivermectin-based mass drug administration (MDA) programs have achieved remarkable success towards the elimination of onchocerciasis and lymphatic filariasis. However, their full implementation has been hindered in Central Africa by the occurrence of ivermectin-related severe adverse events (SAEs) in a subset of individuals with high circulating levels of Loa loa microfilariae. Extending MDA to areas with coincident L. loa infection is problematic, and inexpensive point-of-care tests for L. loa are acutely needed. Herein, we present a lateral flow assay (LFA) to identify subjects with a serological response to Ll-SXP-1, a specific and validated marker of L. loa. The test was evaluated on serum samples from patients infected with L. loa (n = 109) and other helminths (n = 204), as well as on uninfected controls (n = 77). When read with the naked eye, the test was 94% sensitive for L. loa infection and was 100% specific when sera from healthy endemic and non-endemic controls or from those with S. stercoralis infections were used as the comparators. When sera of patients with O. volvulus, W. bancrofti, or M. perstans were used as the comparators, the specificity of the LFA was 82%, 87%, and 88%, respectively. A companion smartphone reader allowed measurement of the test line intensities and establishment of cutoff values. With a cutoff of 600 Units, the assay sensitivity decreased to 71%, but the specificity increased to 96% for O. volvulus, 100% for W. bancrofti, and 100% for M. perstans-infected individuals. The LFA may find applications in refining the current maps of L. loa prevalence, which are needed to eliminate onchocerciasis and lymphatic filariasis from the African continent. |
format |
Article in Journal/Newspaper |
author |
Bijan Pedram Valérie Pasquetto Papa M Drame Yongchang Ji Maria J Gonzalez-Moa Richard K Baldwin Thomas B Nutman Marco A Biamonte |
author_facet |
Bijan Pedram Valérie Pasquetto Papa M Drame Yongchang Ji Maria J Gonzalez-Moa Richard K Baldwin Thomas B Nutman Marco A Biamonte |
author_sort |
Bijan Pedram |
title |
A novel rapid test for detecting antibody responses to Loa loa infections. |
title_short |
A novel rapid test for detecting antibody responses to Loa loa infections. |
title_full |
A novel rapid test for detecting antibody responses to Loa loa infections. |
title_fullStr |
A novel rapid test for detecting antibody responses to Loa loa infections. |
title_full_unstemmed |
A novel rapid test for detecting antibody responses to Loa loa infections. |
title_sort |
novel rapid test for detecting antibody responses to loa loa infections. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005741 https://doaj.org/article/75e896981e0d41149101d542497e8617 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005741 (2017) |
op_relation |
http://europepmc.org/articles/PMC5531435?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005741 https://doaj.org/article/75e896981e0d41149101d542497e8617 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005741 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
7 |
container_start_page |
e0005741 |
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1766343375825403904 |