Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats
Objective: To evaluate the anti-arthritic effect of aqueous and methanolic extracts of Distemonanthus benthamianus. Methods: Monoarthritis was induced by an injection of 0.3 mL zymosan A (0.9% NaCl, v/v) in the right posterior knee joints of rats. Then, joint diameter and pain threshold were determi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2022
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Online Access: | https://doi.org/10.4103/2221-1691.357740 https://doaj.org/article/75afe7d47b2a435b898b0ac56f0e84a3 |
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ftdoajarticles:oai:doaj.org/article:75afe7d47b2a435b898b0ac56f0e84a3 2023-05-15T15:13:43+02:00 Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats William Yousseu Nana Ernest Allah Hoki Kwenteh Gilbert Ateufack Eric Gonzal Tsafack Stephanie Flore Djuichou Nguemnang Zenab Linda Fagni Njoya Albert Donatien Atsamo Vanessa Mba Matah Marthe Carine Flore Adjouzem Yacine Karelle Madjo Kouam Elvira Ngoufack Azanze Marius Mbiantcha 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.357740 https://doaj.org/article/75afe7d47b2a435b898b0ac56f0e84a3 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=10;spage=411;epage=420;aulast=Nana https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.357740 https://doaj.org/article/75afe7d47b2a435b898b0ac56f0e84a3 Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 10, Pp 411-420 (2022) distemonanthus benthamianus rheumatoid arthritis anti-arthritis complete freund’s adjuvant hyperalgesia inflammation oxidative stress Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.357740 2022-12-30T22:40:09Z Objective: To evaluate the anti-arthritic effect of aqueous and methanolic extracts of Distemonanthus benthamianus. Methods: Monoarthritis was induced by an injection of 0.3 mL zymosan A (0.9% NaCl, v/v) in the right posterior knee joints of rats. Then, joint diameter and pain threshold were determined. Polyarthritis was induced by an intracaudal injection of complete Freund’s adjuvant and rats were treated from day 14 post 1st complete Freund’s adjuvant injection until 28 day. The clinical, hematological, biochemical and oxidative stress parameters were evaluated. In addition, histological analysis of the knee joint was perfomed in both tests. Results: The aqueous and methanolic extracts of Distemonanthus benthamianus at a dose of 500 mg/kg ameliorated zymosan A-induced monoarthritis, as evidenced by reduced joint diameter, increased pain threshold, as well as improved joint architecture. In addition, both extracts of Distemonanthus benthamianus markedly increased body weight and pain threshold, while reducing paw edema in polyarthritic rats. They also led to a marked decrease in platelets and white blood cells (P<0.05), as well as a significant increase in red blood cells, hemoglobin and hematocrit (P<0.05). The aqueous and methanolic extracts of Distemonanthus benthamianus significantly reduced alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase activities, while increasing serum protein levels (P<0.05) with no significant variation in creatinine level. Moreover, both extracts increased catalase and glutathione activities (P<0.05), and inhibited malondialdehyde and nitric oxide production (P<0.01 and P<0.001) in the liver and kidneys. Histological analysis of the joints showed that both extracts triggered tissue reparation. Conclusions: Distemonanthus benthamianus could be used as a potential candidate in the treatment of rheumatoid arthritis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 10 411 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
distemonanthus benthamianus rheumatoid arthritis anti-arthritis complete freund’s adjuvant hyperalgesia inflammation oxidative stress Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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distemonanthus benthamianus rheumatoid arthritis anti-arthritis complete freund’s adjuvant hyperalgesia inflammation oxidative stress Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 William Yousseu Nana Ernest Allah Hoki Kwenteh Gilbert Ateufack Eric Gonzal Tsafack Stephanie Flore Djuichou Nguemnang Zenab Linda Fagni Njoya Albert Donatien Atsamo Vanessa Mba Matah Marthe Carine Flore Adjouzem Yacine Karelle Madjo Kouam Elvira Ngoufack Azanze Marius Mbiantcha Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
topic_facet |
distemonanthus benthamianus rheumatoid arthritis anti-arthritis complete freund’s adjuvant hyperalgesia inflammation oxidative stress Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the anti-arthritic effect of aqueous and methanolic extracts of Distemonanthus benthamianus. Methods: Monoarthritis was induced by an injection of 0.3 mL zymosan A (0.9% NaCl, v/v) in the right posterior knee joints of rats. Then, joint diameter and pain threshold were determined. Polyarthritis was induced by an intracaudal injection of complete Freund’s adjuvant and rats were treated from day 14 post 1st complete Freund’s adjuvant injection until 28 day. The clinical, hematological, biochemical and oxidative stress parameters were evaluated. In addition, histological analysis of the knee joint was perfomed in both tests. Results: The aqueous and methanolic extracts of Distemonanthus benthamianus at a dose of 500 mg/kg ameliorated zymosan A-induced monoarthritis, as evidenced by reduced joint diameter, increased pain threshold, as well as improved joint architecture. In addition, both extracts of Distemonanthus benthamianus markedly increased body weight and pain threshold, while reducing paw edema in polyarthritic rats. They also led to a marked decrease in platelets and white blood cells (P<0.05), as well as a significant increase in red blood cells, hemoglobin and hematocrit (P<0.05). The aqueous and methanolic extracts of Distemonanthus benthamianus significantly reduced alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase activities, while increasing serum protein levels (P<0.05) with no significant variation in creatinine level. Moreover, both extracts increased catalase and glutathione activities (P<0.05), and inhibited malondialdehyde and nitric oxide production (P<0.01 and P<0.001) in the liver and kidneys. Histological analysis of the joints showed that both extracts triggered tissue reparation. Conclusions: Distemonanthus benthamianus could be used as a potential candidate in the treatment of rheumatoid arthritis. |
format |
Article in Journal/Newspaper |
author |
William Yousseu Nana Ernest Allah Hoki Kwenteh Gilbert Ateufack Eric Gonzal Tsafack Stephanie Flore Djuichou Nguemnang Zenab Linda Fagni Njoya Albert Donatien Atsamo Vanessa Mba Matah Marthe Carine Flore Adjouzem Yacine Karelle Madjo Kouam Elvira Ngoufack Azanze Marius Mbiantcha |
author_facet |
William Yousseu Nana Ernest Allah Hoki Kwenteh Gilbert Ateufack Eric Gonzal Tsafack Stephanie Flore Djuichou Nguemnang Zenab Linda Fagni Njoya Albert Donatien Atsamo Vanessa Mba Matah Marthe Carine Flore Adjouzem Yacine Karelle Madjo Kouam Elvira Ngoufack Azanze Marius Mbiantcha |
author_sort |
William Yousseu Nana |
title |
Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
title_short |
Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
title_full |
Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
title_fullStr |
Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
title_full_unstemmed |
Anti-arthritic effect of Distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
title_sort |
anti-arthritic effect of distemonanthus benthamianus extracts against rheumatoid arthritis in rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.357740 https://doaj.org/article/75afe7d47b2a435b898b0ac56f0e84a3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 10, Pp 411-420 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=10;spage=411;epage=420;aulast=Nana https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.357740 https://doaj.org/article/75afe7d47b2a435b898b0ac56f0e84a3 |
op_doi |
https://doi.org/10.4103/2221-1691.357740 |
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Asian Pacific Journal of Tropical Biomedicine |
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12 |
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10 |
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411 |
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