Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments?
Over the last decades, exhaled breath sampling has been established for laboratory analysis in various cetacean species. Due to their small size, the usability of respiratory vapor for hormone assessments was questionable in harbor porpoises ( Phocoena phocoena ). This pilot study compared three dif...
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MDPI AG
2021
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ftdoajarticles:oai:doaj.org/article:753874472cf146748b29b2582039e96c 2023-05-15T17:59:08+02:00 Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? Anja Reckendorf Marion Schmicke Paulien Bunskoek Kirstin Anderson Hansen Mette Thybo Christina Strube Ursula Siebert 2021-03-01T00:00:00Z https://doi.org/10.3390/ani11030907 https://doaj.org/article/753874472cf146748b29b2582039e96c EN eng MDPI AG https://www.mdpi.com/2076-2615/11/3/907 https://doaj.org/toc/2076-2615 doi:10.3390/ani11030907 2076-2615 https://doaj.org/article/753874472cf146748b29b2582039e96c Animals, Vol 11, Iss 907, p 907 (2021) harbor porpoise animal welfare non-invasive method exhaled breath blow sampling cortisol Veterinary medicine SF600-1100 Zoology QL1-991 article 2021 ftdoajarticles https://doi.org/10.3390/ani11030907 2022-12-31T09:40:06Z Over the last decades, exhaled breath sampling has been established for laboratory analysis in various cetacean species. Due to their small size, the usability of respiratory vapor for hormone assessments was questionable in harbor porpoises ( Phocoena phocoena ). This pilot study compared three different blow collection devices for their suitability in the field and during laboratory processing: a sterile petri dish covered by a Nitex membrane, as well as sterile 50 mL centrifuge tubes with or without manganese(II) chloride as a stabilizer. Collected exhales varied between three, five or ten, depending on feasibility. Hormones were extracted through an ether mix, followed by centrifugal evaporation and cortisol analysis using an immunoassay. Although close to the lower end of the assay's dynamic range, the ELISA produced results ( n = 110, 0.102–0.937 ng/mL). Hence, a simple 50 mL centrifuge tube was determined as the best suited blow collection device, while three consecutive exhales proved sufficient to yield results. These findings are promising regarding the suitability of exhaled breath as a matrix for future endocrine and immune system-related studies in harbor porpoises. If further advanced, blow sampling can become an important, non-invasive tool for studying and monitoring health, stress levels and diseases in harbor porpoises. Article in Journal/Newspaper Phocoena phocoena Directory of Open Access Journals: DOAJ Articles Animals 11 3 907 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
harbor porpoise animal welfare non-invasive method exhaled breath blow sampling cortisol Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
harbor porpoise animal welfare non-invasive method exhaled breath blow sampling cortisol Veterinary medicine SF600-1100 Zoology QL1-991 Anja Reckendorf Marion Schmicke Paulien Bunskoek Kirstin Anderson Hansen Mette Thybo Christina Strube Ursula Siebert Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? |
topic_facet |
harbor porpoise animal welfare non-invasive method exhaled breath blow sampling cortisol Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Over the last decades, exhaled breath sampling has been established for laboratory analysis in various cetacean species. Due to their small size, the usability of respiratory vapor for hormone assessments was questionable in harbor porpoises ( Phocoena phocoena ). This pilot study compared three different blow collection devices for their suitability in the field and during laboratory processing: a sterile petri dish covered by a Nitex membrane, as well as sterile 50 mL centrifuge tubes with or without manganese(II) chloride as a stabilizer. Collected exhales varied between three, five or ten, depending on feasibility. Hormones were extracted through an ether mix, followed by centrifugal evaporation and cortisol analysis using an immunoassay. Although close to the lower end of the assay's dynamic range, the ELISA produced results ( n = 110, 0.102–0.937 ng/mL). Hence, a simple 50 mL centrifuge tube was determined as the best suited blow collection device, while three consecutive exhales proved sufficient to yield results. These findings are promising regarding the suitability of exhaled breath as a matrix for future endocrine and immune system-related studies in harbor porpoises. If further advanced, blow sampling can become an important, non-invasive tool for studying and monitoring health, stress levels and diseases in harbor porpoises. |
format |
Article in Journal/Newspaper |
author |
Anja Reckendorf Marion Schmicke Paulien Bunskoek Kirstin Anderson Hansen Mette Thybo Christina Strube Ursula Siebert |
author_facet |
Anja Reckendorf Marion Schmicke Paulien Bunskoek Kirstin Anderson Hansen Mette Thybo Christina Strube Ursula Siebert |
author_sort |
Anja Reckendorf |
title |
Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? |
title_short |
Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? |
title_full |
Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? |
title_fullStr |
Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? |
title_full_unstemmed |
Is Harbor Porpoise ( Phocoena phocoena ) Exhaled Breath Sampling Suitable for Hormonal Assessments? |
title_sort |
is harbor porpoise ( phocoena phocoena ) exhaled breath sampling suitable for hormonal assessments? |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ani11030907 https://doaj.org/article/753874472cf146748b29b2582039e96c |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Animals, Vol 11, Iss 907, p 907 (2021) |
op_relation |
https://www.mdpi.com/2076-2615/11/3/907 https://doaj.org/toc/2076-2615 doi:10.3390/ani11030907 2076-2615 https://doaj.org/article/753874472cf146748b29b2582039e96c |
op_doi |
https://doi.org/10.3390/ani11030907 |
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Animals |
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11 |
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3 |
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907 |
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1766167891892240384 |