High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran
Abstract: INTRODUCTION: Plasmid-mediated quinolone resistance (PMQR) is a growing clinical concern worldwide. The main aims of this study were to detect qnr-encoding genes and to evaluate the clonal relatedness of qnr-positive Enterobacter cloacae isolates. METHODS: A total of 116 E. cloacae isolate...
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ftdoajarticles:oai:doaj.org/article:75253a0bb7204b3f93dd67cd237657ea 2023-05-15T15:11:45+02:00 High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran Amir Peymani Taghi Naserpour Farivar Reza Najafipour Samaneh Mansouri 2016-06-01T00:00:00Z https://doi.org/10.1590/0037-8682-0454-2015 https://doaj.org/article/75253a0bb7204b3f93dd67cd237657ea EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000300286&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0454-2015 https://doaj.org/article/75253a0bb7204b3f93dd67cd237657ea Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 3, Pp 286-291 (2016) Enterobacter cloacae Enterobacterial repetitive intergenic consensus-PCR Plasmid-mediated quinolone resistance Arctic medicine. Tropical medicine RC955-962 article 2016 ftdoajarticles https://doi.org/10.1590/0037-8682-0454-2015 2022-12-30T21:27:18Z Abstract: INTRODUCTION: Plasmid-mediated quinolone resistance (PMQR) is a growing clinical concern worldwide. The main aims of this study were to detect qnr-encoding genes and to evaluate the clonal relatedness of qnr-positive Enterobacter cloacae isolates. METHODS: A total of 116 E. cloacae isolates that were not susceptible to quinolone were obtained from seven hospitals in Tehran, five hospitals in Qazvin, and two hospitals in Karaj (Iran). Bacterial identification was performed using standard laboratory methods and API 20E strips. Quinolone resistance was determined using the Kirby-Bauer disk diffusion method according to the Clinical Laboratory Standards Institute (CLSI) guidelines. PCR and sequencing were employed to detect qnrA, qnrB, and qnrS genes, and clonal relatedness was assessed using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method. RESULTS: In total, 45 (38.8%) and 71 (61.2%) of isolates showed high- and low-level quinolone resistance, respectively, and qnr-encoding genes were detected in 70 (60.3%) of them. qnrB1 [45 (38.8%) isolates] was the most commonly detected gene, followed by qnrS1 [28 (24.1%) isolates] and qnrB4 [18 (15.5%) isolates] either alone or in combination with other genes. The results of the ERIC-PCR revealed that 53 (75.7%) qnr-positive isolates were genetically unrelated. CONCLUSIONS: This study describes, for the first time, the high prevalence of the qnrB1, qnrS1, and qnrB4 genes among E. cloacae isolates in Iran. The detection of qnr genes emphasizes the need for establishing tactful policies associated with infection control measures in hospital settings in Iran. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 49 3 286 291 |
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English |
topic |
Enterobacter cloacae Enterobacterial repetitive intergenic consensus-PCR Plasmid-mediated quinolone resistance Arctic medicine. Tropical medicine RC955-962 |
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Enterobacter cloacae Enterobacterial repetitive intergenic consensus-PCR Plasmid-mediated quinolone resistance Arctic medicine. Tropical medicine RC955-962 Amir Peymani Taghi Naserpour Farivar Reza Najafipour Samaneh Mansouri High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran |
topic_facet |
Enterobacter cloacae Enterobacterial repetitive intergenic consensus-PCR Plasmid-mediated quinolone resistance Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract: INTRODUCTION: Plasmid-mediated quinolone resistance (PMQR) is a growing clinical concern worldwide. The main aims of this study were to detect qnr-encoding genes and to evaluate the clonal relatedness of qnr-positive Enterobacter cloacae isolates. METHODS: A total of 116 E. cloacae isolates that were not susceptible to quinolone were obtained from seven hospitals in Tehran, five hospitals in Qazvin, and two hospitals in Karaj (Iran). Bacterial identification was performed using standard laboratory methods and API 20E strips. Quinolone resistance was determined using the Kirby-Bauer disk diffusion method according to the Clinical Laboratory Standards Institute (CLSI) guidelines. PCR and sequencing were employed to detect qnrA, qnrB, and qnrS genes, and clonal relatedness was assessed using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method. RESULTS: In total, 45 (38.8%) and 71 (61.2%) of isolates showed high- and low-level quinolone resistance, respectively, and qnr-encoding genes were detected in 70 (60.3%) of them. qnrB1 [45 (38.8%) isolates] was the most commonly detected gene, followed by qnrS1 [28 (24.1%) isolates] and qnrB4 [18 (15.5%) isolates] either alone or in combination with other genes. The results of the ERIC-PCR revealed that 53 (75.7%) qnr-positive isolates were genetically unrelated. CONCLUSIONS: This study describes, for the first time, the high prevalence of the qnrB1, qnrS1, and qnrB4 genes among E. cloacae isolates in Iran. The detection of qnr genes emphasizes the need for establishing tactful policies associated with infection control measures in hospital settings in Iran. |
format |
Article in Journal/Newspaper |
author |
Amir Peymani Taghi Naserpour Farivar Reza Najafipour Samaneh Mansouri |
author_facet |
Amir Peymani Taghi Naserpour Farivar Reza Najafipour Samaneh Mansouri |
author_sort |
Amir Peymani |
title |
High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran |
title_short |
High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran |
title_full |
High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran |
title_fullStr |
High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran |
title_full_unstemmed |
High prevalence of plasmid-mediated quinolone resistance determinants in Enterobacter cloacae isolated from hospitals of the Qazvin, Alborz, and Tehran provinces, Iran |
title_sort |
high prevalence of plasmid-mediated quinolone resistance determinants in enterobacter cloacae isolated from hospitals of the qazvin, alborz, and tehran provinces, iran |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2016 |
url |
https://doi.org/10.1590/0037-8682-0454-2015 https://doaj.org/article/75253a0bb7204b3f93dd67cd237657ea |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 3, Pp 286-291 (2016) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000300286&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0454-2015 https://doaj.org/article/75253a0bb7204b3f93dd67cd237657ea |
op_doi |
https://doi.org/10.1590/0037-8682-0454-2015 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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49 |
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286 |
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