Plasma proteomics data from hibernating and active Scandinavian brown bears
In this article, we present mass-spectrometry based plasma proteomics data from hibernating and active free-ranging Scandinavian brown bears (Ursus arctos).The brown bear hibernates for half the year. Despite obesity when entering the den and the prolonged period of inactivity, the bear shows no sig...
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ftdoajarticles:oai:doaj.org/article:7510bc2ecf444b269e65ab0062d5d5c1 2023-05-15T18:41:50+02:00 Plasma proteomics data from hibernating and active Scandinavian brown bears Anne Mette Frøbert Simon Gregersen Malene Brohus Karen G. Welinder Jonas Kindberg Ole Fröbert Michael T. Overgaard 2022-04-01T00:00:00Z https://doi.org/10.1016/j.dib.2022.107959 https://doaj.org/article/7510bc2ecf444b269e65ab0062d5d5c1 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2352340922001706 https://doaj.org/toc/2352-3409 2352-3409 doi:10.1016/j.dib.2022.107959 https://doaj.org/article/7510bc2ecf444b269e65ab0062d5d5c1 Data in Brief, Vol 41, Iss , Pp 107959- (2022) Ursus arctos Hibernation Proteins Blood Mass spectrometry Translational medicine Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 article 2022 ftdoajarticles https://doi.org/10.1016/j.dib.2022.107959 2022-12-30T22:42:43Z In this article, we present mass-spectrometry based plasma proteomics data from hibernating and active free-ranging Scandinavian brown bears (Ursus arctos).The brown bear hibernates for half the year. Despite obesity when entering the den and the prolonged period of inactivity, the bear shows no signs of the harmful effects associated with these conditions in humans. Thus, the hibernating bear is a potential translational model for addressing these complications in humans.We analyzed plasma samples from fourteen 2- to 3-year-old bears (6 males and 8 females) collected both during hibernation and the active state, and for some of the bears during two seasons, resulting in a total of 38 analyzed plasma samples.In triplicates, the plasma proteins were unfolded and reduced. To increase the chance of detecting low-molecular-weight proteins and peptides, we filtered the samples using a 50 K molecular weight cut-off filter with the aim to deplete larger abundant proteins, including albumin, and thereby increase the depth of the analysis. The proteins in the permeate were then tryptically digested, desalted, and analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed with the MaxQuant software searching against an Ursus arctos horribilis protein database.Here, we provide the raw data, a list with identified proteins in the plasma samples, and the databases applied for protein identification.Based on the provided data, differentially expressed proteins in hibernation compared to active state can be identified. These proteins may be involved in the bears’ adaptions to hibernation physiology and hold potential as novel therapeutic targets. Article in Journal/Newspaper Ursus arctos Directory of Open Access Journals: DOAJ Articles Data in Brief 41 107959 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ursus arctos Hibernation Proteins Blood Mass spectrometry Translational medicine Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 |
spellingShingle |
Ursus arctos Hibernation Proteins Blood Mass spectrometry Translational medicine Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 Anne Mette Frøbert Simon Gregersen Malene Brohus Karen G. Welinder Jonas Kindberg Ole Fröbert Michael T. Overgaard Plasma proteomics data from hibernating and active Scandinavian brown bears |
topic_facet |
Ursus arctos Hibernation Proteins Blood Mass spectrometry Translational medicine Computer applications to medicine. Medical informatics R858-859.7 Science (General) Q1-390 |
description |
In this article, we present mass-spectrometry based plasma proteomics data from hibernating and active free-ranging Scandinavian brown bears (Ursus arctos).The brown bear hibernates for half the year. Despite obesity when entering the den and the prolonged period of inactivity, the bear shows no signs of the harmful effects associated with these conditions in humans. Thus, the hibernating bear is a potential translational model for addressing these complications in humans.We analyzed plasma samples from fourteen 2- to 3-year-old bears (6 males and 8 females) collected both during hibernation and the active state, and for some of the bears during two seasons, resulting in a total of 38 analyzed plasma samples.In triplicates, the plasma proteins were unfolded and reduced. To increase the chance of detecting low-molecular-weight proteins and peptides, we filtered the samples using a 50 K molecular weight cut-off filter with the aim to deplete larger abundant proteins, including albumin, and thereby increase the depth of the analysis. The proteins in the permeate were then tryptically digested, desalted, and analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed with the MaxQuant software searching against an Ursus arctos horribilis protein database.Here, we provide the raw data, a list with identified proteins in the plasma samples, and the databases applied for protein identification.Based on the provided data, differentially expressed proteins in hibernation compared to active state can be identified. These proteins may be involved in the bears’ adaptions to hibernation physiology and hold potential as novel therapeutic targets. |
format |
Article in Journal/Newspaper |
author |
Anne Mette Frøbert Simon Gregersen Malene Brohus Karen G. Welinder Jonas Kindberg Ole Fröbert Michael T. Overgaard |
author_facet |
Anne Mette Frøbert Simon Gregersen Malene Brohus Karen G. Welinder Jonas Kindberg Ole Fröbert Michael T. Overgaard |
author_sort |
Anne Mette Frøbert |
title |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_short |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_full |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_fullStr |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_full_unstemmed |
Plasma proteomics data from hibernating and active Scandinavian brown bears |
title_sort |
plasma proteomics data from hibernating and active scandinavian brown bears |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.dib.2022.107959 https://doaj.org/article/7510bc2ecf444b269e65ab0062d5d5c1 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Data in Brief, Vol 41, Iss , Pp 107959- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2352340922001706 https://doaj.org/toc/2352-3409 2352-3409 doi:10.1016/j.dib.2022.107959 https://doaj.org/article/7510bc2ecf444b269e65ab0062d5d5c1 |
op_doi |
https://doi.org/10.1016/j.dib.2022.107959 |
container_title |
Data in Brief |
container_volume |
41 |
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107959 |
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1766231391268241408 |