Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe
Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: Toxoplasma gondii , Neospora caninum and microsporidia E...
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ftdoajarticles:oai:doaj.org/article:750e69607f89439ebb8c65b4c925ebdd 2023-07-23T04:21:04+02:00 Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka 2023-06-01T00:00:00Z https://doi.org/10.3390/ijms24129887 https://doaj.org/article/750e69607f89439ebb8c65b4c925ebdd EN eng MDPI AG https://www.mdpi.com/1422-0067/24/12/9887 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms24129887 1422-0067 1661-6596 https://doaj.org/article/750e69607f89439ebb8c65b4c925ebdd International Journal of Molecular Sciences, Vol 24, Iss 9887, p 9887 (2023) insectivore microsporidiosis molecular methods neosporosis toxoplasmosis Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms24129887 2023-07-02T00:38:10Z Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: Toxoplasma gondii , Neospora caninum and microsporidia Encephalitozoon spp. In total, brain and small intestine tissues of 100 bats (52 Myotis myotis , 43 Nyctalus noctula and 5 Vespertilio murinus ) were used for the DNA isolation and PCR detection of the abovementioned agents. Toxoplasma gondii DNA was detected by real-time PCR in 1% of bats (in one male of M. myotis ), while all bats were negative for N. caninum DNA. Encephalitozoon spp. DNA was detected by nested PCR in 25% of bats, including three species (twenty-two M. myotis , two N. noctula and one V. murinus ). Positive samples were sequenced and showed homology with the genotypes Encephalitozoon cuniculi II and Encephalitozoon hellem 2C. This is the first study on wild vespertilionid bats from Central Europe and worldwide, with a relatively high positivity of Encephalitozoon spp. detected in bats. Article in Journal/Newspaper Nyctalus noctula Vespertilio murinus Directory of Open Access Journals: DOAJ Articles Hellem ENVELOPE(9.604,9.604,63.780,63.780) International Journal of Molecular Sciences 24 12 9887 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
insectivore microsporidiosis molecular methods neosporosis toxoplasmosis Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
insectivore microsporidiosis molecular methods neosporosis toxoplasmosis Biology (General) QH301-705.5 Chemistry QD1-999 Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe |
topic_facet |
insectivore microsporidiosis molecular methods neosporosis toxoplasmosis Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: Toxoplasma gondii , Neospora caninum and microsporidia Encephalitozoon spp. In total, brain and small intestine tissues of 100 bats (52 Myotis myotis , 43 Nyctalus noctula and 5 Vespertilio murinus ) were used for the DNA isolation and PCR detection of the abovementioned agents. Toxoplasma gondii DNA was detected by real-time PCR in 1% of bats (in one male of M. myotis ), while all bats were negative for N. caninum DNA. Encephalitozoon spp. DNA was detected by nested PCR in 25% of bats, including three species (twenty-two M. myotis , two N. noctula and one V. murinus ). Positive samples were sequenced and showed homology with the genotypes Encephalitozoon cuniculi II and Encephalitozoon hellem 2C. This is the first study on wild vespertilionid bats from Central Europe and worldwide, with a relatively high positivity of Encephalitozoon spp. detected in bats. |
format |
Article in Journal/Newspaper |
author |
Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka |
author_facet |
Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka |
author_sort |
Eva Bártová |
title |
Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe |
title_short |
Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe |
title_full |
Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe |
title_fullStr |
Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe |
title_full_unstemmed |
Molecular Detection of Toxoplasma gondii , Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe |
title_sort |
molecular detection of toxoplasma gondii , neospora caninum and encephalitozoon spp. in vespertilionid bats from central europe |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms24129887 https://doaj.org/article/750e69607f89439ebb8c65b4c925ebdd |
long_lat |
ENVELOPE(9.604,9.604,63.780,63.780) |
geographic |
Hellem |
geographic_facet |
Hellem |
genre |
Nyctalus noctula Vespertilio murinus |
genre_facet |
Nyctalus noctula Vespertilio murinus |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 9887, p 9887 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/12/9887 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms24129887 1422-0067 1661-6596 https://doaj.org/article/750e69607f89439ebb8c65b4c925ebdd |
op_doi |
https://doi.org/10.3390/ijms24129887 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
12 |
container_start_page |
9887 |
_version_ |
1772186248352366592 |