A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas
A single and practical method to slain Malassezia furfur and Corynebacterium minutissimum in lesions' scales is described. The scales are collected by pressing small pieces of scotch tape (about 4 cm lenght and 2 cm width) onto the lesions and following withdrawl the furfuraceous scales will re...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Language: | English |
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Universidade de São Paulo (USP)
1996
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Online Access: | https://doi.org/10.1590/S0036-46651996000400011 https://doaj.org/article/75081b2e48d342069b2d7c06093d066b |
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ftdoajarticles:oai:doaj.org/article:75081b2e48d342069b2d7c06093d066b 2024-09-09T19:28:33+00:00 A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas Antar Padilha-Gonçalves 1996-08-01T00:00:00Z https://doi.org/10.1590/S0036-46651996000400011 https://doaj.org/article/75081b2e48d342069b2d7c06093d066b EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000400011 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000400011 0036-4665 1678-9946 https://doaj.org/article/75081b2e48d342069b2d7c06093d066b Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 4, Pp 299-302 (1996) Corynebacterium minutissimum Pityriasis versicolor Erythrasma Staining method Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1996 ftdoajarticles https://doi.org/10.1590/S0036-46651996000400011 2024-08-05T17:49:30Z A single and practical method to slain Malassezia furfur and Corynebacterium minutissimum in lesions' scales is described. The scales are collected by pressing small pieces of scotch tape (about 4 cm lenght and 2 cm width) onto the lesions and following withdrawl the furfuraceous scales will remain on the glue side. These pieces are then immersed for some minutes in lactophenol-cotton blue stain. Following absorption of the stain the scales are washed in current water to remove the excess of blue stain, dried with filter paper, dehydrated via passage in two bottles containing absolute alcohol and then placed in xylene in a centrifugation tube. The xylene dissolves the scotch tape glue and the scales fall free in the tube. After centrifugation and decantation the scales concentrated on the bottom of the tube are collected with a platinum-loop, placed in Canada balsam on a microscopy slide and closed with a cover slip. The preparations are then ready to be submitted to microscopic examination. Other stains may also be used instead of lactophenol-cotton blue. This method is simple, easily performed, and offers good conditions to study these fungi as well as being useful for the diagnosis of the diseases that they cause. É descrito um método simples e prático para corar Malassezia furfur e Corynebacterium minutissimum nas escamas das lesões. O material é colhido com o auxílio de fita durex que será usada na maior parte das etapas do método para ajudar a fácil execução do processo de coloração. Para colher as escamas, pequenos pedaços de fita durex com cerca de 4 cm de comprimento por 2 cm de largura são colocados e pressionados sobre as lesões, e quando retirados trazem aderidas as escamas furfuráceas na face com goma. Esses pedaços de fita durex são imersos por alguns minutos no corante lactofenol-azul cotton e logo que as escamas estiverem coradas em azul são lavadas em água corrente para remover o excesso de corante azul, secos com papel de filtro, desidratados pela passagem em dois frascos com álcool absoluto e ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Canada Revista do Instituto de Medicina Tropical de São Paulo 38 4 299 302 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Corynebacterium minutissimum Pityriasis versicolor Erythrasma Staining method Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Corynebacterium minutissimum Pityriasis versicolor Erythrasma Staining method Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Antar Padilha-Gonçalves A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas |
topic_facet |
Corynebacterium minutissimum Pityriasis versicolor Erythrasma Staining method Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
A single and practical method to slain Malassezia furfur and Corynebacterium minutissimum in lesions' scales is described. The scales are collected by pressing small pieces of scotch tape (about 4 cm lenght and 2 cm width) onto the lesions and following withdrawl the furfuraceous scales will remain on the glue side. These pieces are then immersed for some minutes in lactophenol-cotton blue stain. Following absorption of the stain the scales are washed in current water to remove the excess of blue stain, dried with filter paper, dehydrated via passage in two bottles containing absolute alcohol and then placed in xylene in a centrifugation tube. The xylene dissolves the scotch tape glue and the scales fall free in the tube. After centrifugation and decantation the scales concentrated on the bottom of the tube are collected with a platinum-loop, placed in Canada balsam on a microscopy slide and closed with a cover slip. The preparations are then ready to be submitted to microscopic examination. Other stains may also be used instead of lactophenol-cotton blue. This method is simple, easily performed, and offers good conditions to study these fungi as well as being useful for the diagnosis of the diseases that they cause. É descrito um método simples e prático para corar Malassezia furfur e Corynebacterium minutissimum nas escamas das lesões. O material é colhido com o auxílio de fita durex que será usada na maior parte das etapas do método para ajudar a fácil execução do processo de coloração. Para colher as escamas, pequenos pedaços de fita durex com cerca de 4 cm de comprimento por 2 cm de largura são colocados e pressionados sobre as lesões, e quando retirados trazem aderidas as escamas furfuráceas na face com goma. Esses pedaços de fita durex são imersos por alguns minutos no corante lactofenol-azul cotton e logo que as escamas estiverem coradas em azul são lavadas em água corrente para remover o excesso de corante azul, secos com papel de filtro, desidratados pela passagem em dois frascos com álcool absoluto e ... |
format |
Article in Journal/Newspaper |
author |
Antar Padilha-Gonçalves |
author_facet |
Antar Padilha-Gonçalves |
author_sort |
Antar Padilha-Gonçalves |
title |
A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas |
title_short |
A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas |
title_full |
A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas |
title_fullStr |
A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas |
title_full_unstemmed |
A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales Um método simples para corar Malassezia furfur e Corynebacterium minutissimum nas escamas |
title_sort |
single method to stain malassezia furfur and corynebacterium minutissimum in scales um método simples para corar malassezia furfur e corynebacterium minutissimum nas escamas |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1996 |
url |
https://doi.org/10.1590/S0036-46651996000400011 https://doaj.org/article/75081b2e48d342069b2d7c06093d066b |
geographic |
Arctic Canada |
geographic_facet |
Arctic Canada |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 4, Pp 299-302 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000400011 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000400011 0036-4665 1678-9946 https://doaj.org/article/75081b2e48d342069b2d7c06093d066b |
op_doi |
https://doi.org/10.1590/S0036-46651996000400011 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
38 |
container_issue |
4 |
container_start_page |
299 |
op_container_end_page |
302 |
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1809897865571467264 |