Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models
Abstract Background Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important. Methods E...
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ftdoajarticles:oai:doaj.org/article:74fb8d76c555459199e9a2c7951514c9 2023-05-15T15:51:01+02:00 Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models Ratanaporn Tangwangvivat Supassama Chaiyawong Nutthawan Nonthabenjawan Kamonpan Charoenkul Taveesak Janethanakit Kitikhun Udom Sawang Kesdangsakonwut Rachod Tantilertcharoen Aunyaratana Thontiravong Alongkorn Amonsin 2022-10-01T00:00:00Z https://doi.org/10.1186/s12985-022-01888-x https://doaj.org/article/74fb8d76c555459199e9a2c7951514c9 EN eng BMC https://doi.org/10.1186/s12985-022-01888-x https://doaj.org/toc/1743-422X doi:10.1186/s12985-022-01888-x 1743-422X https://doaj.org/article/74fb8d76c555459199e9a2c7951514c9 Virology Journal, Vol 19, Iss 1, Pp 1-12 (2022) Canine influenza Dog Guinea pigs H3N2 Pathogenicity Transmission Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12985-022-01888-x 2022-12-30T21:32:42Z Abstract Background Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important. Methods Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 106 EID50 per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 106 EID50 per ml of CIV-H3N2. Results For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1–2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed. Conclusion The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Virology Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Canine influenza Dog Guinea pigs H3N2 Pathogenicity Transmission Infectious and parasitic diseases RC109-216 |
spellingShingle |
Canine influenza Dog Guinea pigs H3N2 Pathogenicity Transmission Infectious and parasitic diseases RC109-216 Ratanaporn Tangwangvivat Supassama Chaiyawong Nutthawan Nonthabenjawan Kamonpan Charoenkul Taveesak Janethanakit Kitikhun Udom Sawang Kesdangsakonwut Rachod Tantilertcharoen Aunyaratana Thontiravong Alongkorn Amonsin Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models |
topic_facet |
Canine influenza Dog Guinea pigs H3N2 Pathogenicity Transmission Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important. Methods Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 106 EID50 per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 106 EID50 per ml of CIV-H3N2. Results For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1–2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed. Conclusion The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model. |
format |
Article in Journal/Newspaper |
author |
Ratanaporn Tangwangvivat Supassama Chaiyawong Nutthawan Nonthabenjawan Kamonpan Charoenkul Taveesak Janethanakit Kitikhun Udom Sawang Kesdangsakonwut Rachod Tantilertcharoen Aunyaratana Thontiravong Alongkorn Amonsin |
author_facet |
Ratanaporn Tangwangvivat Supassama Chaiyawong Nutthawan Nonthabenjawan Kamonpan Charoenkul Taveesak Janethanakit Kitikhun Udom Sawang Kesdangsakonwut Rachod Tantilertcharoen Aunyaratana Thontiravong Alongkorn Amonsin |
author_sort |
Ratanaporn Tangwangvivat |
title |
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models |
title_short |
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models |
title_full |
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models |
title_fullStr |
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models |
title_full_unstemmed |
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models |
title_sort |
transmission and pathogenicity of canine h3n2 influenza virus in dog and guinea pig models |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12985-022-01888-x https://doaj.org/article/74fb8d76c555459199e9a2c7951514c9 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Virology Journal, Vol 19, Iss 1, Pp 1-12 (2022) |
op_relation |
https://doi.org/10.1186/s12985-022-01888-x https://doaj.org/toc/1743-422X doi:10.1186/s12985-022-01888-x 1743-422X https://doaj.org/article/74fb8d76c555459199e9a2c7951514c9 |
op_doi |
https://doi.org/10.1186/s12985-022-01888-x |
container_title |
Virology Journal |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766386063461318656 |