Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus
Although it has been demonstrated that venoms and toxins from some snakes are able to influence the growth of tumor cells, few antitumoral compounds from Bothrops leucurus venom have been characterized. Leucurolysin-B (leuc-B) is a metalloproteinase class P-III isolated from B. leucurus which posses...
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ftdoajarticles:oai:doaj.org/article:74e76f3588dc4624996a48cf71c0795a 2023-05-15T15:07:37+02:00 Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus LM Gabriel EF Sanchez SG Silva RG Santos 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000100004 https://doaj.org/article/74e76f3588dc4624996a48cf71c0795a EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000100004 1678-9199 https://doaj.org/article/74e76f3588dc4624996a48cf71c0795a Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 1, Pp 24-33 (2012) snake venom leucurolysin-B ECD-disintegrin tumor in vitro study Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000100004 2022-12-31T14:09:37Z Although it has been demonstrated that venoms and toxins from some snakes are able to influence the growth of tumor cells, few antitumoral compounds from Bothrops leucurus venom have been characterized. Leucurolysin-B (leuc-B) is a metalloproteinase class P-III isolated from B. leucurus which possesses an ECD-disintegrin domain. Both ECD-disentegrin and RGD-disintegrin are able to bind to cell surface integrins and inhibit their adherence to their natural ligands. In the present study, the potential efficacy and the cytotoxic effects of leuc-B on glioblastoma, breast cancer and melanoma cell lines were analyzed. The effect of leuc-B on cancer cell survival was evaluated and its 50% inhibitory concentration (IC50) was determined. Morphological alterations were monitored by contrast phase and fluorescent microscopy. The results demonstrated that leuc-B has potent cytotoxic effect in a micromolar range against all evaluated cancer cell lines. Morphologically, dying cells showed fragmentation, condensation of their contents concomitant with shrinkage and appearance of vacuoles. This study reports for the first time the cytotoxic effect of leuc-B from B. leucurus snake venom on tumor cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 1 24 33 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
snake venom leucurolysin-B ECD-disintegrin tumor in vitro study Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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snake venom leucurolysin-B ECD-disintegrin tumor in vitro study Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 LM Gabriel EF Sanchez SG Silva RG Santos Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus |
topic_facet |
snake venom leucurolysin-B ECD-disintegrin tumor in vitro study Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Although it has been demonstrated that venoms and toxins from some snakes are able to influence the growth of tumor cells, few antitumoral compounds from Bothrops leucurus venom have been characterized. Leucurolysin-B (leuc-B) is a metalloproteinase class P-III isolated from B. leucurus which possesses an ECD-disintegrin domain. Both ECD-disentegrin and RGD-disintegrin are able to bind to cell surface integrins and inhibit their adherence to their natural ligands. In the present study, the potential efficacy and the cytotoxic effects of leuc-B on glioblastoma, breast cancer and melanoma cell lines were analyzed. The effect of leuc-B on cancer cell survival was evaluated and its 50% inhibitory concentration (IC50) was determined. Morphological alterations were monitored by contrast phase and fluorescent microscopy. The results demonstrated that leuc-B has potent cytotoxic effect in a micromolar range against all evaluated cancer cell lines. Morphologically, dying cells showed fragmentation, condensation of their contents concomitant with shrinkage and appearance of vacuoles. This study reports for the first time the cytotoxic effect of leuc-B from B. leucurus snake venom on tumor cells. |
format |
Article in Journal/Newspaper |
author |
LM Gabriel EF Sanchez SG Silva RG Santos |
author_facet |
LM Gabriel EF Sanchez SG Silva RG Santos |
author_sort |
LM Gabriel |
title |
Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus |
title_short |
Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus |
title_full |
Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus |
title_fullStr |
Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus |
title_full_unstemmed |
Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus |
title_sort |
tumor cytotoxicity of leucurolysin-b, a p-iii snake venom metalloproteinase from bothrops leucurus |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000100004 https://doaj.org/article/74e76f3588dc4624996a48cf71c0795a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 1, Pp 24-33 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000100004 1678-9199 https://doaj.org/article/74e76f3588dc4624996a48cf71c0795a |
op_doi |
https://doi.org/10.1590/S1678-91992012000100004 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
18 |
container_issue |
1 |
container_start_page |
24 |
op_container_end_page |
33 |
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1766339084893028352 |