Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon
We report the development of a multigene gene expression assay on the BioMark HD platform for the evaluation of immune competence (ImCom) in farmed Atlantic salmon. The first version of the assay included 92 genes selected on the basis of transcriptome analyses in 54 trials that challenged the immun...
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2020
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ftdoajarticles:oai:doaj.org/article:74e299c644c74941851ee287a225bebe 2023-05-15T15:30:17+02:00 Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon Aleksei Krasnov Sergey Afanasyev Stian Nylund Alexander Rebl 2020-10-01T00:00:00Z https://doi.org/10.3390/genes11111236 https://doaj.org/article/74e299c644c74941851ee287a225bebe EN eng MDPI AG https://www.mdpi.com/2073-4425/11/11/1236 https://doaj.org/toc/2073-4425 doi:10.3390/genes11111236 2073-4425 https://doaj.org/article/74e299c644c74941851ee287a225bebe Genes, Vol 11, Iss 1236, p 1236 (2020) Atlantic salmon smolt gene expression immune competence Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.3390/genes11111236 2022-12-31T15:56:29Z We report the development of a multigene gene expression assay on the BioMark HD platform for the evaluation of immune competence (ImCom) in farmed Atlantic salmon. The first version of the assay included 92 genes selected on the basis of transcriptome analyses in 54 trials that challenged the immune system; annotations were taken into account to represent the key pathways of innate and adaptive immunity. ImCom was tested on samples collected from seven independent projects. Fish were reared from the start feeding to eight months in the sea at eight units in different parts of Norway. Several tissues were analyzed. Linear discriminant analysis (LDA) showed that no more than 10 genes were required to separate groups, and a set of 46 immune genes was sufficient for any task. The second version of the assay was tested in the gills of two groups of high-performing healthy smolts and in groups with intermediate and high mortality rates (IM and HM, respectively). A set of 645 gill samples from clinically healthy Atlantic salmon was used as a reference. The IM group showed general suppression of immunity. All HM group salmon were above the threshold by the squared deviation from the reference. This group showed marked upregulation of genes involved in acute stress and inflammation: mmp-9, mmp-13, hsp70, il-1b, lect2 , and cathelicidin . Further work will clarify the boundaries of the norm and explore various cases of impaired immunity. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Norway Genes 11 11 1236 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon smolt gene expression immune competence Genetics QH426-470 |
spellingShingle |
Atlantic salmon smolt gene expression immune competence Genetics QH426-470 Aleksei Krasnov Sergey Afanasyev Stian Nylund Alexander Rebl Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon |
topic_facet |
Atlantic salmon smolt gene expression immune competence Genetics QH426-470 |
description |
We report the development of a multigene gene expression assay on the BioMark HD platform for the evaluation of immune competence (ImCom) in farmed Atlantic salmon. The first version of the assay included 92 genes selected on the basis of transcriptome analyses in 54 trials that challenged the immune system; annotations were taken into account to represent the key pathways of innate and adaptive immunity. ImCom was tested on samples collected from seven independent projects. Fish were reared from the start feeding to eight months in the sea at eight units in different parts of Norway. Several tissues were analyzed. Linear discriminant analysis (LDA) showed that no more than 10 genes were required to separate groups, and a set of 46 immune genes was sufficient for any task. The second version of the assay was tested in the gills of two groups of high-performing healthy smolts and in groups with intermediate and high mortality rates (IM and HM, respectively). A set of 645 gill samples from clinically healthy Atlantic salmon was used as a reference. The IM group showed general suppression of immunity. All HM group salmon were above the threshold by the squared deviation from the reference. This group showed marked upregulation of genes involved in acute stress and inflammation: mmp-9, mmp-13, hsp70, il-1b, lect2 , and cathelicidin . Further work will clarify the boundaries of the norm and explore various cases of impaired immunity. |
format |
Article in Journal/Newspaper |
author |
Aleksei Krasnov Sergey Afanasyev Stian Nylund Alexander Rebl |
author_facet |
Aleksei Krasnov Sergey Afanasyev Stian Nylund Alexander Rebl |
author_sort |
Aleksei Krasnov |
title |
Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon |
title_short |
Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon |
title_full |
Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon |
title_fullStr |
Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon |
title_full_unstemmed |
Multigene Expression Assay for Assessment of the Immune Status of Atlantic Salmon |
title_sort |
multigene expression assay for assessment of the immune status of atlantic salmon |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/genes11111236 https://doaj.org/article/74e299c644c74941851ee287a225bebe |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Genes, Vol 11, Iss 1236, p 1236 (2020) |
op_relation |
https://www.mdpi.com/2073-4425/11/11/1236 https://doaj.org/toc/2073-4425 doi:10.3390/genes11111236 2073-4425 https://doaj.org/article/74e299c644c74941851ee287a225bebe |
op_doi |
https://doi.org/10.3390/genes11111236 |
container_title |
Genes |
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11 |
container_issue |
11 |
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1236 |
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1766360737430634496 |