Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo
Objective: To evaluate the combination therapy of pyronaridine tetraphosphate and diminazene aceturate against Babesia in vitro and in vivo. Methods: Bioinformatic analysis was performed using atom pair fingerprints. An in vitro combination test was performed against Babesia bovis and Theileria equi...
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Wolters Kluwer Medknow Publications
2024
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Online Access: | https://doi.org/10.4103/apjtb.apjtb_887_23 https://doaj.org/article/74e204ef4dec40508fd4ce5b4cba792c |
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ftdoajarticles:oai:doaj.org/article:74e204ef4dec40508fd4ce5b4cba792c 2024-09-09T19:26:13+00:00 Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo Shimaa Abd El-Salam El-Sayed Mohamed Z Sayed-Ahmed Shaimaa Ahmed Awad Ali Nourah Alsadaan Nawazish Alam Mahmoud S Alkhoudary Ikuo Igarashi Mohamed Abdo Rizk 2024-01-01T00:00:00Z https://doi.org/10.4103/apjtb.apjtb_887_23 https://doaj.org/article/74e204ef4dec40508fd4ce5b4cba792c EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=3;spage=93;epage=99;aulast=El-Sayed https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/apjtb.apjtb_887_23 https://doaj.org/article/74e204ef4dec40508fd4ce5b4cba792c Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 3, Pp 93-99 (2024) babesia pyronaridine tetraphosphate diminazene aceturate babesiosis theileria Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2024 ftdoajarticles https://doi.org/10.4103/apjtb.apjtb_887_23 2024-08-05T17:49:31Z Objective: To evaluate the combination therapy of pyronaridine tetraphosphate and diminazene aceturate against Babesia in vitro and in vivo. Methods: Bioinformatic analysis was performed using atom pair fingerprints. An in vitro combination test was performed against Babesia bovis and Theileria equi. Moreover, the in vivo chemotherapeutic efficacy of pyronaridine tetraphosphate in combination with diminazene aceturate was investigated against the growth of Babesia microti in mice using a fluorescence inhibitory assay. Results: Pyronaridine tetraphosphate and diminazene aceturate exhibited nearly similar molecular weights. The in vitro combination of pyronaridine tetraphosphate and diminazene aceturate was synergistic on Babesia bovis and additive on Theileria equi. In addition, 5 mg/kg pyronaridine tetraphosphate combined with 10 mg/kg diminazene aceturate inhibited Babesia microti growth significantly compared with those observed after treatment with 25 mg/kg diminazene aceturate alone from day 6 post treatment to day 12 post treatment. The combination therapy also normalized the hematological parameters of infected mice. Conclusions: An oral dose of pyronaridine tetraphosphate combined with a subcutaneous dose of diminazene aceturate inhibits Babesia in vitro and in mice, suggesting it might be a new paradigm for the treatment of babesiosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 14 3 93 99 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
babesia pyronaridine tetraphosphate diminazene aceturate babesiosis theileria Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
babesia pyronaridine tetraphosphate diminazene aceturate babesiosis theileria Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Shimaa Abd El-Salam El-Sayed Mohamed Z Sayed-Ahmed Shaimaa Ahmed Awad Ali Nourah Alsadaan Nawazish Alam Mahmoud S Alkhoudary Ikuo Igarashi Mohamed Abdo Rizk Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo |
topic_facet |
babesia pyronaridine tetraphosphate diminazene aceturate babesiosis theileria Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the combination therapy of pyronaridine tetraphosphate and diminazene aceturate against Babesia in vitro and in vivo. Methods: Bioinformatic analysis was performed using atom pair fingerprints. An in vitro combination test was performed against Babesia bovis and Theileria equi. Moreover, the in vivo chemotherapeutic efficacy of pyronaridine tetraphosphate in combination with diminazene aceturate was investigated against the growth of Babesia microti in mice using a fluorescence inhibitory assay. Results: Pyronaridine tetraphosphate and diminazene aceturate exhibited nearly similar molecular weights. The in vitro combination of pyronaridine tetraphosphate and diminazene aceturate was synergistic on Babesia bovis and additive on Theileria equi. In addition, 5 mg/kg pyronaridine tetraphosphate combined with 10 mg/kg diminazene aceturate inhibited Babesia microti growth significantly compared with those observed after treatment with 25 mg/kg diminazene aceturate alone from day 6 post treatment to day 12 post treatment. The combination therapy also normalized the hematological parameters of infected mice. Conclusions: An oral dose of pyronaridine tetraphosphate combined with a subcutaneous dose of diminazene aceturate inhibits Babesia in vitro and in mice, suggesting it might be a new paradigm for the treatment of babesiosis. |
format |
Article in Journal/Newspaper |
author |
Shimaa Abd El-Salam El-Sayed Mohamed Z Sayed-Ahmed Shaimaa Ahmed Awad Ali Nourah Alsadaan Nawazish Alam Mahmoud S Alkhoudary Ikuo Igarashi Mohamed Abdo Rizk |
author_facet |
Shimaa Abd El-Salam El-Sayed Mohamed Z Sayed-Ahmed Shaimaa Ahmed Awad Ali Nourah Alsadaan Nawazish Alam Mahmoud S Alkhoudary Ikuo Igarashi Mohamed Abdo Rizk |
author_sort |
Shimaa Abd El-Salam El-Sayed |
title |
Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo |
title_short |
Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo |
title_full |
Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo |
title_fullStr |
Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo |
title_full_unstemmed |
Pyronaridine combined with diminazene aceturate inhibits Babesia in vitro and in vivo |
title_sort |
pyronaridine combined with diminazene aceturate inhibits babesia in vitro and in vivo |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2024 |
url |
https://doi.org/10.4103/apjtb.apjtb_887_23 https://doaj.org/article/74e204ef4dec40508fd4ce5b4cba792c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 3, Pp 93-99 (2024) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=3;spage=93;epage=99;aulast=El-Sayed https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/apjtb.apjtb_887_23 https://doaj.org/article/74e204ef4dec40508fd4ce5b4cba792c |
op_doi |
https://doi.org/10.4103/apjtb.apjtb_887_23 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
14 |
container_issue |
3 |
container_start_page |
93 |
op_container_end_page |
99 |
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1809895881432891392 |