Obesity impairs resistance to Leishmania major infection in C57BL/6 mice.
An association between increased susceptibility to infectious diseases and obesity has been described as a result of impaired immunity in obese individuals. It is not clear whether a similar linkage can be drawn between obesity and parasitic diseases. To evaluate the effect of obesity in the immune...
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ftdoajarticles:oai:doaj.org/article:74e1cf2e88c340abb31e1088e676158b 2023-05-15T15:11:15+02:00 Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. Vinicius Dantas Martins Franciele Carolina Silva Felipe Caixeta Matheus Batista Carneiro Graziele Ribeiro Goes Lícia Torres Sara Cândida Barbosa Leonardo Vaz Nivea Carolina Paiva Cláudia Martins Carneiro Leda Quercia Vieira Ana Maria Caetano Faria Tatiani Uceli Maioli 2020-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006596 https://doaj.org/article/74e1cf2e88c340abb31e1088e676158b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0006596 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006596 https://doaj.org/article/74e1cf2e88c340abb31e1088e676158b PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0006596 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006596 2022-12-31T07:16:57Z An association between increased susceptibility to infectious diseases and obesity has been described as a result of impaired immunity in obese individuals. It is not clear whether a similar linkage can be drawn between obesity and parasitic diseases. To evaluate the effect of obesity in the immune response to cutaneous Leishmania major infection, we studied the ability of C57BL/6 mice fed a hypercaloric diet (HSB) to control leishmaniasis. Mice with diet-induced obesity presented thicker lesions with higher parasite burden and a more intense inflammatory infiltrate in the infected ear after infection with L. major. There was no difference between control and obese mice in IFN-gamma or IL-4 production by auricular draining lymph node cells, but obese mice produced higher levels of IgG1 and IL-17. Peritoneal macrophages from obese mice were less efficient to kill L. major when infected in vitro than macrophages from control mice. In vitro stimulation of macrophages with IL-17 decreased their capacity to kill the parasite. Moreover, macrophages from obese mice presented higher arginase activity. To confirm the role of IL-17 in the context of obesity and infection, we studied lesion development in obese IL-17R-/- mice infected with L. major and found no difference in skin lesions and the leukocyte accumulation in the draining lymph node is redcuced in knockout mice compared between obese and lean animals. Our results indicate that diet-induced obesity impairs resistance to L. major in C57BL/6 mice and that IL-17 is involved in lesion development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 1 e0006596 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vinicius Dantas Martins Franciele Carolina Silva Felipe Caixeta Matheus Batista Carneiro Graziele Ribeiro Goes Lícia Torres Sara Cândida Barbosa Leonardo Vaz Nivea Carolina Paiva Cláudia Martins Carneiro Leda Quercia Vieira Ana Maria Caetano Faria Tatiani Uceli Maioli Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
An association between increased susceptibility to infectious diseases and obesity has been described as a result of impaired immunity in obese individuals. It is not clear whether a similar linkage can be drawn between obesity and parasitic diseases. To evaluate the effect of obesity in the immune response to cutaneous Leishmania major infection, we studied the ability of C57BL/6 mice fed a hypercaloric diet (HSB) to control leishmaniasis. Mice with diet-induced obesity presented thicker lesions with higher parasite burden and a more intense inflammatory infiltrate in the infected ear after infection with L. major. There was no difference between control and obese mice in IFN-gamma or IL-4 production by auricular draining lymph node cells, but obese mice produced higher levels of IgG1 and IL-17. Peritoneal macrophages from obese mice were less efficient to kill L. major when infected in vitro than macrophages from control mice. In vitro stimulation of macrophages with IL-17 decreased their capacity to kill the parasite. Moreover, macrophages from obese mice presented higher arginase activity. To confirm the role of IL-17 in the context of obesity and infection, we studied lesion development in obese IL-17R-/- mice infected with L. major and found no difference in skin lesions and the leukocyte accumulation in the draining lymph node is redcuced in knockout mice compared between obese and lean animals. Our results indicate that diet-induced obesity impairs resistance to L. major in C57BL/6 mice and that IL-17 is involved in lesion development. |
format |
Article in Journal/Newspaper |
author |
Vinicius Dantas Martins Franciele Carolina Silva Felipe Caixeta Matheus Batista Carneiro Graziele Ribeiro Goes Lícia Torres Sara Cândida Barbosa Leonardo Vaz Nivea Carolina Paiva Cláudia Martins Carneiro Leda Quercia Vieira Ana Maria Caetano Faria Tatiani Uceli Maioli |
author_facet |
Vinicius Dantas Martins Franciele Carolina Silva Felipe Caixeta Matheus Batista Carneiro Graziele Ribeiro Goes Lícia Torres Sara Cândida Barbosa Leonardo Vaz Nivea Carolina Paiva Cláudia Martins Carneiro Leda Quercia Vieira Ana Maria Caetano Faria Tatiani Uceli Maioli |
author_sort |
Vinicius Dantas Martins |
title |
Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. |
title_short |
Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. |
title_full |
Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. |
title_fullStr |
Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. |
title_full_unstemmed |
Obesity impairs resistance to Leishmania major infection in C57BL/6 mice. |
title_sort |
obesity impairs resistance to leishmania major infection in c57bl/6 mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0006596 https://doaj.org/article/74e1cf2e88c340abb31e1088e676158b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0006596 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0006596 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006596 https://doaj.org/article/74e1cf2e88c340abb31e1088e676158b |
op_doi |
https://doi.org/10.1371/journal.pntd.0006596 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
1 |
container_start_page |
e0006596 |
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1766342139593097216 |