Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense.
The effect of the dinoflagellate, Alexandrium fundyense, on relative expression of glutathione S-transferase (GST) transcripts was examined in the copepod Calanus finmarchicus. Adult females were fed for 5-days on one of three experimental diets: control (100% Rhodomonas spp.), low dose of A. fundye...
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ftdoajarticles:oai:doaj.org/article:74c25eae56804f50a0da4564a3546929 2023-05-15T15:47:57+02:00 Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. Vittoria Roncalli Michelle J Jungbluth Petra H Lenz 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0159563 https://doaj.org/article/74c25eae56804f50a0da4564a3546929 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4948837?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0159563 https://doaj.org/article/74c25eae56804f50a0da4564a3546929 PLoS ONE, Vol 11, Iss 7, p e0159563 (2016) Medicine R Science Q article 2016 ftdoajarticles https://doi.org/10.1371/journal.pone.0159563 2022-12-31T13:49:38Z The effect of the dinoflagellate, Alexandrium fundyense, on relative expression of glutathione S-transferase (GST) transcripts was examined in the copepod Calanus finmarchicus. Adult females were fed for 5-days on one of three experimental diets: control (100% Rhodomonas spp.), low dose of A. fundyense (25% by volume, 75% Rhodomonas spp.), and high dose (100% A. fundyense). Relative expression of three GST genes was measured using RT-qPCR on days 0.5, 1, 2 and 5 in two independent experiments. Differential regulation was found for the Delta and the Sigma GSTs between 0.5 to 2 days, but not on day 5 in both experiments. The third GST, a microsomal, was not differentially expressed in either treatment or day. RT-qPCR results from the two experiments were similar, even though experimental females were collected from the Gulf of Maine on different dates and their reproductive output differed. In the second experiment, expression of 39 GSTs was determined on days 2 and 5 using RNA-Seq. Global gene expression analyses agreed with the RT-qPCR results. Furthermore, the RNA-Seq measurements indicated that only four GSTs were differentially expressed under the experimental conditions, and the response was small in amplitude. In summary, the A. fundyense diet led to a rapid and transient response in C. finmarchicus in three cytosolic GSTs, while a fourth GST (Omega I) was significantly up-regulated on day 5. Although there was some regulation of GSTs in response the toxic dinoflagellate, the tolerance to A. fundyense by C. finmarchicus is not dependent on the long-term up-regulation of specific GSTs. Article in Journal/Newspaper Calanus finmarchicus Directory of Open Access Journals: DOAJ Articles PLOS ONE 11 7 e0159563 |
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Medicine R Science Q Vittoria Roncalli Michelle J Jungbluth Petra H Lenz Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. |
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Medicine R Science Q |
description |
The effect of the dinoflagellate, Alexandrium fundyense, on relative expression of glutathione S-transferase (GST) transcripts was examined in the copepod Calanus finmarchicus. Adult females were fed for 5-days on one of three experimental diets: control (100% Rhodomonas spp.), low dose of A. fundyense (25% by volume, 75% Rhodomonas spp.), and high dose (100% A. fundyense). Relative expression of three GST genes was measured using RT-qPCR on days 0.5, 1, 2 and 5 in two independent experiments. Differential regulation was found for the Delta and the Sigma GSTs between 0.5 to 2 days, but not on day 5 in both experiments. The third GST, a microsomal, was not differentially expressed in either treatment or day. RT-qPCR results from the two experiments were similar, even though experimental females were collected from the Gulf of Maine on different dates and their reproductive output differed. In the second experiment, expression of 39 GSTs was determined on days 2 and 5 using RNA-Seq. Global gene expression analyses agreed with the RT-qPCR results. Furthermore, the RNA-Seq measurements indicated that only four GSTs were differentially expressed under the experimental conditions, and the response was small in amplitude. In summary, the A. fundyense diet led to a rapid and transient response in C. finmarchicus in three cytosolic GSTs, while a fourth GST (Omega I) was significantly up-regulated on day 5. Although there was some regulation of GSTs in response the toxic dinoflagellate, the tolerance to A. fundyense by C. finmarchicus is not dependent on the long-term up-regulation of specific GSTs. |
format |
Article in Journal/Newspaper |
author |
Vittoria Roncalli Michelle J Jungbluth Petra H Lenz |
author_facet |
Vittoria Roncalli Michelle J Jungbluth Petra H Lenz |
author_sort |
Vittoria Roncalli |
title |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. |
title_short |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. |
title_full |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. |
title_fullStr |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. |
title_full_unstemmed |
Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense. |
title_sort |
glutathione s-transferase regulation in calanus finmarchicus feeding on the toxic dinoflagellate alexandrium fundyense. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0159563 https://doaj.org/article/74c25eae56804f50a0da4564a3546929 |
genre |
Calanus finmarchicus |
genre_facet |
Calanus finmarchicus |
op_source |
PLoS ONE, Vol 11, Iss 7, p e0159563 (2016) |
op_relation |
http://europepmc.org/articles/PMC4948837?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0159563 https://doaj.org/article/74c25eae56804f50a0da4564a3546929 |
op_doi |
https://doi.org/10.1371/journal.pone.0159563 |
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PLOS ONE |
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11 |
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7 |
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e0159563 |
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