A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes
Abstract Background The ability to culture Plasmodium falciparum continuously in vitro has enabled stable access to asexual and sexual parasites for malaria research. The portfolio of isolates has remained limited and research is still largely based on NF54 and its derived clone 3D7. Since 1978, iso...
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ftdoajarticles:oai:doaj.org/article:74833f3d5a7348b299bf409cd6b49e04 2023-05-15T15:15:42+02:00 A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes Marga van de Vegte-Bolmer Wouter Graumans Rianne Stoter Geert-Jan van Gemert Robert Sauerwein Katharine A. Collins Teun Bousema 2021-09-01T00:00:00Z https://doi.org/10.1186/s12936-021-03912-x https://doaj.org/article/74833f3d5a7348b299bf409cd6b49e04 EN eng BMC https://doi.org/10.1186/s12936-021-03912-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03912-x 1475-2875 https://doaj.org/article/74833f3d5a7348b299bf409cd6b49e04 Malaria Journal, Vol 20, Iss 1, Pp 1-10 (2021) Malaria Transmission Parasite culture Culture adaptation Cloning Clinical isolates Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03912-x 2022-12-31T07:15:07Z Abstract Background The ability to culture Plasmodium falciparum continuously in vitro has enabled stable access to asexual and sexual parasites for malaria research. The portfolio of isolates has remained limited and research is still largely based on NF54 and its derived clone 3D7. Since 1978, isolates were collected and cryopreserved at Radboudumc from patients presenting at the hospital. Here, procedures are described for culture adaptation of asexual parasites, cloning and production of sexual stage parasites responsible for transmission (gametocytes) and production of oocysts in Anopheles mosquitoes. This study aimed to identify new culture-adapted transmissible P. falciparum isolates, originating from distinct geographical locations. Methods Out of a collection of 121 P. falciparum isolates stored in liquid nitrogen, 21 from different geographical origin were selected for initial testing. Isolates were evaluated for their ability to be asexually cultured in vitro, their gametocyte production capacity, and consistent generation of oocysts. Results Out of 21 isolates tested, twelve were excluded from further analysis due to lack of mature gametocyte production (n = 1) or generation of satisfactory numbers of oocysts in mosquitoes (n = 11). Nine isolates fulfilled selection criteria and were cloned by limiting dilution and retested. After cloning, one isolate was excluded for not showing transmission. The remaining eight isolates transmitted to Anopheles stephensi or Anopheles coluzzii mosquitoes and were categorized into two groups with a reproducible mean oocyst infection intensity above (n = 5) or below five (n = 3). Conclusions These new P. falciparum culture-adapted isolates with reproducible transmission to Anopheles mosquitoes are a valuable addition to the malaria research tool box. They can aid in the development of malaria interventions and will be particularly useful for those studying malaria transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Transmission Parasite culture Culture adaptation Cloning Clinical isolates Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Transmission Parasite culture Culture adaptation Cloning Clinical isolates Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Marga van de Vegte-Bolmer Wouter Graumans Rianne Stoter Geert-Jan van Gemert Robert Sauerwein Katharine A. Collins Teun Bousema A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes |
topic_facet |
Malaria Transmission Parasite culture Culture adaptation Cloning Clinical isolates Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The ability to culture Plasmodium falciparum continuously in vitro has enabled stable access to asexual and sexual parasites for malaria research. The portfolio of isolates has remained limited and research is still largely based on NF54 and its derived clone 3D7. Since 1978, isolates were collected and cryopreserved at Radboudumc from patients presenting at the hospital. Here, procedures are described for culture adaptation of asexual parasites, cloning and production of sexual stage parasites responsible for transmission (gametocytes) and production of oocysts in Anopheles mosquitoes. This study aimed to identify new culture-adapted transmissible P. falciparum isolates, originating from distinct geographical locations. Methods Out of a collection of 121 P. falciparum isolates stored in liquid nitrogen, 21 from different geographical origin were selected for initial testing. Isolates were evaluated for their ability to be asexually cultured in vitro, their gametocyte production capacity, and consistent generation of oocysts. Results Out of 21 isolates tested, twelve were excluded from further analysis due to lack of mature gametocyte production (n = 1) or generation of satisfactory numbers of oocysts in mosquitoes (n = 11). Nine isolates fulfilled selection criteria and were cloned by limiting dilution and retested. After cloning, one isolate was excluded for not showing transmission. The remaining eight isolates transmitted to Anopheles stephensi or Anopheles coluzzii mosquitoes and were categorized into two groups with a reproducible mean oocyst infection intensity above (n = 5) or below five (n = 3). Conclusions These new P. falciparum culture-adapted isolates with reproducible transmission to Anopheles mosquitoes are a valuable addition to the malaria research tool box. They can aid in the development of malaria interventions and will be particularly useful for those studying malaria transmission. |
format |
Article in Journal/Newspaper |
author |
Marga van de Vegte-Bolmer Wouter Graumans Rianne Stoter Geert-Jan van Gemert Robert Sauerwein Katharine A. Collins Teun Bousema |
author_facet |
Marga van de Vegte-Bolmer Wouter Graumans Rianne Stoter Geert-Jan van Gemert Robert Sauerwein Katharine A. Collins Teun Bousema |
author_sort |
Marga van de Vegte-Bolmer |
title |
A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes |
title_short |
A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes |
title_full |
A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes |
title_fullStr |
A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes |
title_full_unstemmed |
A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes |
title_sort |
portfolio of geographically distinct laboratory-adapted plasmodium falciparum clones with consistent infection rates in anopheles mosquitoes |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03912-x https://doaj.org/article/74833f3d5a7348b299bf409cd6b49e04 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-10 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03912-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03912-x 1475-2875 https://doaj.org/article/74833f3d5a7348b299bf409cd6b49e04 |
op_doi |
https://doi.org/10.1186/s12936-021-03912-x |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766346053262508032 |