Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis
Abstract INTRODUCTION: Cutaneous leishmaniasis is caused by protozoa of the genus Leishmania and transmission occurs through the bite of sandflies. It is an infectious disease, which affects skin and mucosa. The aim was to quantify the macrophages M1 and M2 and the annexin A1 expression in the skin...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1590/0037-8682-0361-2019 https://doaj.org/article/7466a37cfc2e49a9b35d373589bcad4d |
id |
ftdoajarticles:oai:doaj.org/article:7466a37cfc2e49a9b35d373589bcad4d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:7466a37cfc2e49a9b35d373589bcad4d 2023-05-15T15:10:37+02:00 Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis Joselina Maria da Silva Helen Aguiar Lemes da Silva Carolina Zelenski Jéssica Azevedo de Moraes Souza Marcia Hueb Amílcar Sabino Damazo 2019-12-01T00:00:00Z https://doi.org/10.1590/0037-8682-0361-2019 https://doaj.org/article/7466a37cfc2e49a9b35d373589bcad4d EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100348&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0361-2019 https://doaj.org/article/7466a37cfc2e49a9b35d373589bcad4d Revista da Sociedade Brasileira de Medicina Tropical, Vol 52 (2019) Cutaneous leishmaniasis Annexin A1 Macrophages Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.1590/0037-8682-0361-2019 2022-12-31T02:52:39Z Abstract INTRODUCTION: Cutaneous leishmaniasis is caused by protozoa of the genus Leishmania and transmission occurs through the bite of sandflies. It is an infectious disease, which affects skin and mucosa. The aim was to quantify the macrophages M1 and M2 and the annexin A1 expression in the skin lesions of patients with cutaneous leishmaniasis. METHODS: Skin biopsies from patients (n = 50) were analyzed and classified according to the lesion type as: exudative cellular reaction, exudative granulomatous reaction, exudative necrotic reaction, exudative necrotic-granulomatous reaction. Using the immunofluorescence technique, macrophages were identified by CD163 marker, differentiated by anti-MHCII and anti-CD206 antibodies, and annexin A1 expression was determined by arbitrary unit (a.u.) densitometry. RESULTS: In M1 macrophages, a greater expression of this protein was observed in the exudative cellular reaction type lesions (136.3 ± 2.6 a.u., assuming mean and standard derivation) when compared to the expression in the lesions of exudative granulomatous reaction, exudative necrotic reaction and exudative necrotic-granulomatous reaction patients (108.0 ± 2.3, 121.6 ± 3.2 and 124.7 ± 2.4 a.u., respectively). Regarding M2 macrophages, it was observed that patients with exudative cellular reaction lesion also had a higher expression of this protein (128.8 ± 2.6 a.u.), when compared to the expression in the lesions of exudative granulomatous reaction, exudative necrotic reaction and exudative necrotic-granulomatous reaction patients (105.6 ± 2, 113.9 ± 2.8, 114.3 ± 2.1 a.u., respectively). CONCLUSIONS: These data suggest that annexin A1 is assisting macrophages in the phagocytosis process of patients with exudative cellular reaction lesion type. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 52 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cutaneous leishmaniasis Annexin A1 Macrophages Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Cutaneous leishmaniasis Annexin A1 Macrophages Arctic medicine. Tropical medicine RC955-962 Joselina Maria da Silva Helen Aguiar Lemes da Silva Carolina Zelenski Jéssica Azevedo de Moraes Souza Marcia Hueb Amílcar Sabino Damazo Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis |
topic_facet |
Cutaneous leishmaniasis Annexin A1 Macrophages Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: Cutaneous leishmaniasis is caused by protozoa of the genus Leishmania and transmission occurs through the bite of sandflies. It is an infectious disease, which affects skin and mucosa. The aim was to quantify the macrophages M1 and M2 and the annexin A1 expression in the skin lesions of patients with cutaneous leishmaniasis. METHODS: Skin biopsies from patients (n = 50) were analyzed and classified according to the lesion type as: exudative cellular reaction, exudative granulomatous reaction, exudative necrotic reaction, exudative necrotic-granulomatous reaction. Using the immunofluorescence technique, macrophages were identified by CD163 marker, differentiated by anti-MHCII and anti-CD206 antibodies, and annexin A1 expression was determined by arbitrary unit (a.u.) densitometry. RESULTS: In M1 macrophages, a greater expression of this protein was observed in the exudative cellular reaction type lesions (136.3 ± 2.6 a.u., assuming mean and standard derivation) when compared to the expression in the lesions of exudative granulomatous reaction, exudative necrotic reaction and exudative necrotic-granulomatous reaction patients (108.0 ± 2.3, 121.6 ± 3.2 and 124.7 ± 2.4 a.u., respectively). Regarding M2 macrophages, it was observed that patients with exudative cellular reaction lesion also had a higher expression of this protein (128.8 ± 2.6 a.u.), when compared to the expression in the lesions of exudative granulomatous reaction, exudative necrotic reaction and exudative necrotic-granulomatous reaction patients (105.6 ± 2, 113.9 ± 2.8, 114.3 ± 2.1 a.u., respectively). CONCLUSIONS: These data suggest that annexin A1 is assisting macrophages in the phagocytosis process of patients with exudative cellular reaction lesion type. |
format |
Article in Journal/Newspaper |
author |
Joselina Maria da Silva Helen Aguiar Lemes da Silva Carolina Zelenski Jéssica Azevedo de Moraes Souza Marcia Hueb Amílcar Sabino Damazo |
author_facet |
Joselina Maria da Silva Helen Aguiar Lemes da Silva Carolina Zelenski Jéssica Azevedo de Moraes Souza Marcia Hueb Amílcar Sabino Damazo |
author_sort |
Joselina Maria da Silva |
title |
Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis |
title_short |
Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis |
title_full |
Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis |
title_fullStr |
Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis |
title_full_unstemmed |
Analysis of macrophage subtypes and annexin A1 expression in lesions of patients with cutaneous leishmaniasis |
title_sort |
analysis of macrophage subtypes and annexin a1 expression in lesions of patients with cutaneous leishmaniasis |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2019 |
url |
https://doi.org/10.1590/0037-8682-0361-2019 https://doaj.org/article/7466a37cfc2e49a9b35d373589bcad4d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 52 (2019) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100348&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0361-2019 https://doaj.org/article/7466a37cfc2e49a9b35d373589bcad4d |
op_doi |
https://doi.org/10.1590/0037-8682-0361-2019 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
52 |
_version_ |
1766341612594528256 |