Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children
Abstract Background Even though malaria is generally on the decline due extensive control and elimination efforts, it still remains a public health problem for over 40% of the world’s population. During the course of malaria infection, parasites and red blood cells come under oxidative stress and th...
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ftdoajarticles:oai:doaj.org/article:7452b8906c9f4165907c1e4c82f9223a 2023-05-15T15:17:59+02:00 Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children Samuel Antwi-Baffour Ransford Kyeremeh Dorcas Buabeng Jonathan Kofi Adjei Claudia Aryeh George Kpentey Mahmood Abdulai Seidu 2018-11-01T00:00:00Z https://doi.org/10.1186/s12936-018-2569-x https://doaj.org/article/7452b8906c9f4165907c1e4c82f9223a EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2569-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2569-x 1475-2875 https://doaj.org/article/7452b8906c9f4165907c1e4c82f9223a Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) Monocytes Lymphocytes Parasitaemia Malaria and peripheral blood Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2569-x 2022-12-31T14:37:24Z Abstract Background Even though malaria is generally on the decline due extensive control and elimination efforts, it still remains a public health problem for over 40% of the world’s population. During the course of malaria infection, parasites and red blood cells come under oxidative stress and there is host immune response in an attempt to protect the red blood cells. The frequency of monocytes and lymphocytes in peripheral blood might, therefore, be expected to reflect the state of an individual’s immune response to the infection. Circulating monocytes and lymphocytes could therefore serve as an index in relation to malaria parasitaemia. The purpose of this study was to determine whether the relative count of monocytes to lymphocytes in peripheral blood (M:L ratio) can predict parasitaemia and, therefore, the severity of malaria infection. Methods Two millilitre of venous blood sample were taken from participants by venisection into anticoagulant tubes. Thick and thin blood films were made and stained with Giemsa and examined for malaria parasites. Whole blood specimen were analysed for full blood count using ABX Pentra 60 C+ automated haematological analyzer. Data was entered into Microsoft Word and analysed using Statistical Package for Social Sciences (SPSS, Version 20.0) and Graphpad prism. Spearman’s correlation was used to determine correlation between occurrences of clinical malaria and the monocytes and lymphocytes ratio. Statistical significance was taken as p ≤ 0.05 with 95% confidence interval. Results The study comprised of 1629 (m = 896; f = 733) children up to 5 years presenting with clinical malaria as cases and 445 (m = 257; f = 188) apparently healthy children as controls. The results indicated that there was a significant positive correlation between the monocytes to lymphocytes ratio and the presence of parasites (p = 0.04) and the level of parasitaemia within the age group of 0–3 years (p = 0.02) and 4–5 years (p = 0.03). Conclusions The monocyte to lymphocyte ratio obtained correlated ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Monocytes Lymphocytes Parasitaemia Malaria and peripheral blood Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Monocytes Lymphocytes Parasitaemia Malaria and peripheral blood Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Samuel Antwi-Baffour Ransford Kyeremeh Dorcas Buabeng Jonathan Kofi Adjei Claudia Aryeh George Kpentey Mahmood Abdulai Seidu Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children |
topic_facet |
Monocytes Lymphocytes Parasitaemia Malaria and peripheral blood Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Even though malaria is generally on the decline due extensive control and elimination efforts, it still remains a public health problem for over 40% of the world’s population. During the course of malaria infection, parasites and red blood cells come under oxidative stress and there is host immune response in an attempt to protect the red blood cells. The frequency of monocytes and lymphocytes in peripheral blood might, therefore, be expected to reflect the state of an individual’s immune response to the infection. Circulating monocytes and lymphocytes could therefore serve as an index in relation to malaria parasitaemia. The purpose of this study was to determine whether the relative count of monocytes to lymphocytes in peripheral blood (M:L ratio) can predict parasitaemia and, therefore, the severity of malaria infection. Methods Two millilitre of venous blood sample were taken from participants by venisection into anticoagulant tubes. Thick and thin blood films were made and stained with Giemsa and examined for malaria parasites. Whole blood specimen were analysed for full blood count using ABX Pentra 60 C+ automated haematological analyzer. Data was entered into Microsoft Word and analysed using Statistical Package for Social Sciences (SPSS, Version 20.0) and Graphpad prism. Spearman’s correlation was used to determine correlation between occurrences of clinical malaria and the monocytes and lymphocytes ratio. Statistical significance was taken as p ≤ 0.05 with 95% confidence interval. Results The study comprised of 1629 (m = 896; f = 733) children up to 5 years presenting with clinical malaria as cases and 445 (m = 257; f = 188) apparently healthy children as controls. The results indicated that there was a significant positive correlation between the monocytes to lymphocytes ratio and the presence of parasites (p = 0.04) and the level of parasitaemia within the age group of 0–3 years (p = 0.02) and 4–5 years (p = 0.03). Conclusions The monocyte to lymphocyte ratio obtained correlated ... |
format |
Article in Journal/Newspaper |
author |
Samuel Antwi-Baffour Ransford Kyeremeh Dorcas Buabeng Jonathan Kofi Adjei Claudia Aryeh George Kpentey Mahmood Abdulai Seidu |
author_facet |
Samuel Antwi-Baffour Ransford Kyeremeh Dorcas Buabeng Jonathan Kofi Adjei Claudia Aryeh George Kpentey Mahmood Abdulai Seidu |
author_sort |
Samuel Antwi-Baffour |
title |
Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children |
title_short |
Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children |
title_full |
Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children |
title_fullStr |
Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children |
title_full_unstemmed |
Correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in Ghanaian children |
title_sort |
correlation of malaria parasitaemia with peripheral blood monocyte to lymphocyte ratio as indicator of susceptibility to severe malaria in ghanaian children |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2569-x https://doaj.org/article/7452b8906c9f4165907c1e4c82f9223a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2569-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2569-x 1475-2875 https://doaj.org/article/7452b8906c9f4165907c1e4c82f9223a |
op_doi |
https://doi.org/10.1186/s12936-018-2569-x |
container_title |
Malaria Journal |
container_volume |
17 |
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1 |
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1766348220691120128 |