Discovery of mosquito saliva microRNAs during CHIKV infection.
Mosquito borne pathogens are transmitted to humans via saliva during blood feeding. Mosquito saliva is a complex concoction of many secretory factors that modulate the feeding foci to enhance pathogen infection and establishment. Multiple salivary proteins/factors have been identified/characterized...
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ftdoajarticles:oai:doaj.org/article:7428553dd6cd4d54984d66e29622e38f 2023-05-15T15:06:25+02:00 Discovery of mosquito saliva microRNAs during CHIKV infection. Payal D Maharaj Steven G Widen Jing Huang Thomas G Wood Saravanan Thangamani 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003386 https://doaj.org/article/7428553dd6cd4d54984d66e29622e38f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4303268?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003386 https://doaj.org/article/7428553dd6cd4d54984d66e29622e38f PLoS Neglected Tropical Diseases, Vol 9, Iss 1, p e0003386 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003386 2022-12-31T00:49:30Z Mosquito borne pathogens are transmitted to humans via saliva during blood feeding. Mosquito saliva is a complex concoction of many secretory factors that modulate the feeding foci to enhance pathogen infection and establishment. Multiple salivary proteins/factors have been identified/characterized that enhance pathogen infection. Here, we describe, for the first time, the identification of exogenous microRNAs from mosquito saliva. MicroRNAs are short, 18-24 nucleotide, non-coding RNAs that regulate gene expression, and are generally intracellular. However, circulating miRNAs have been described from serum and saliva of humans. Exogenous miRNAs have not been reported from hematophagous arthropod saliva. We sought to identify miRNAs in the mosquito saliva and their role in Chikungunya virus (CHIKV) infection. Next generation sequencing was utilized to identify 103 exogenous miRNAs in mosquito saliva of which 31 miRNAs were previously unidentified and were designated novel. Several miRNAs that we have identified are expressed only in the CHIKV infected mosquitoes. Five of the saliva miRNAs were tested for their potential to regulated CHIKV infection, and our results demonstrate their functional role in the transmission and establishment of infection during blood feeding on the host. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 1 e0003386 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Payal D Maharaj Steven G Widen Jing Huang Thomas G Wood Saravanan Thangamani Discovery of mosquito saliva microRNAs during CHIKV infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mosquito borne pathogens are transmitted to humans via saliva during blood feeding. Mosquito saliva is a complex concoction of many secretory factors that modulate the feeding foci to enhance pathogen infection and establishment. Multiple salivary proteins/factors have been identified/characterized that enhance pathogen infection. Here, we describe, for the first time, the identification of exogenous microRNAs from mosquito saliva. MicroRNAs are short, 18-24 nucleotide, non-coding RNAs that regulate gene expression, and are generally intracellular. However, circulating miRNAs have been described from serum and saliva of humans. Exogenous miRNAs have not been reported from hematophagous arthropod saliva. We sought to identify miRNAs in the mosquito saliva and their role in Chikungunya virus (CHIKV) infection. Next generation sequencing was utilized to identify 103 exogenous miRNAs in mosquito saliva of which 31 miRNAs were previously unidentified and were designated novel. Several miRNAs that we have identified are expressed only in the CHIKV infected mosquitoes. Five of the saliva miRNAs were tested for their potential to regulated CHIKV infection, and our results demonstrate their functional role in the transmission and establishment of infection during blood feeding on the host. |
format |
Article in Journal/Newspaper |
author |
Payal D Maharaj Steven G Widen Jing Huang Thomas G Wood Saravanan Thangamani |
author_facet |
Payal D Maharaj Steven G Widen Jing Huang Thomas G Wood Saravanan Thangamani |
author_sort |
Payal D Maharaj |
title |
Discovery of mosquito saliva microRNAs during CHIKV infection. |
title_short |
Discovery of mosquito saliva microRNAs during CHIKV infection. |
title_full |
Discovery of mosquito saliva microRNAs during CHIKV infection. |
title_fullStr |
Discovery of mosquito saliva microRNAs during CHIKV infection. |
title_full_unstemmed |
Discovery of mosquito saliva microRNAs during CHIKV infection. |
title_sort |
discovery of mosquito saliva micrornas during chikv infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003386 https://doaj.org/article/7428553dd6cd4d54984d66e29622e38f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 1, p e0003386 (2015) |
op_relation |
http://europepmc.org/articles/PMC4303268?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003386 https://doaj.org/article/7428553dd6cd4d54984d66e29622e38f |
op_doi |
https://doi.org/10.1371/journal.pntd.0003386 |
container_title |
PLOS Neglected Tropical Diseases |
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9 |
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1 |
container_start_page |
e0003386 |
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1766338029485555712 |