Nueva técnica difásica en tubo para el diagnóstico de estrongiloidiasis

• Main Authors: Maldonado Adriana, Rivero de Rodríguez Zulbey, Bracho Angela, Avila Ayarí.
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2018
• Subjects: Parásitos intestinales; Strongyloides stercoralis; estrongiloidiasis; técnica de agar en placa; técnica difásica en tubo; Intestinal parasites; S. stercoralis; strongyloidiasis; agar plate technique; diphasic tube technique; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic; Ritchie
• Online Access: https://doaj.org/article/740a72b6f84b422f8ca66937e1b37608

Abstract: A study of enteroparasites in an indigenous population was carried out, through which a new diagnostic procedure for strongyloidiasis, diphasic technique in tube (DTT), was proposed, in order to compare it with plaque agar (Arakaki). The methodology consisted in the analysis of fecal samples of 50 individuals by means of a fresh test, Ritchie concentration technique, plate agar technique and the DTT proposed here. A high parasitic prevalence and poliparasitism was obtained, with 94% and 70% respectively. Blastocystis spp. (chromist) prevailed, followed by Giardia intestinalis, among the protozoa, while among the helminths the geohelmities predominated, headed by Trichuris trichiura and Strongyloides stercoralis. The sensitivity and specificity of DTT was 75% and 66.7%, respectively, at 24 hours of reading, the species being isolated 1.5 times more on the traditional technique, and 85.71% and 16.67% at 48 hours, recovering the nematode 1.6 times more, showing greater sensitivity. No cases of strongyloidiasis were reported through the fresh examination, nor by the Ritchie technique. It is concluded that DTT, is more effective for diagnosis of the species S. stercoralis, both at 24 and at 48 hours of reading. Resumen: Se realizó un estudio de enteroparásitos en una población indígena, a través del cual se propuso un nuevo procedimiento de diagnóstico para estrongiloidiasis, técnica difásica en tubo (TDT), con el objeto de compararla con la de agar en placa (Arakaki). La metodología consistió en análisis de muestras fecales de 50 individuos mediante examen al fresco, técnica de concentración de Ritchie, técnica de agar en placa y la TDT aquí propuesta. Se obtuvo una prevalencia parasitaria y poliparasitismo elevados, con 94% y 70% respectivamente. Blastocystis spp. (chromista) prevaleció, seguido de Giardia intestinalis, entre los protozoarios, mientras entre los helmintos predominaron los geohelmitos, encabezados por Trichuris trichiura y Strongyloides stercoralis. La sensibilidad y especificidad de la ...