Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Lisandra Serra Damasceno, Tania Vite-Garín, José Antonio Ramírez, Gabriela Rodríguez-Arellanes, Marcos Abreu de Almeida, Mauro de Medeiros Muniz, Jacó Ricarte Lima de Mesquita, Terezinha do Menino Jesus Silva Leitão, Maria Lucia Taylor, Rosely Maria Zancopé-Oliveira
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2019
• Subjects: Mixed infection; Histoplasma capsulatum; Mating types; Multilocus sequence typing; Histoplasmosis; HIV coinfections; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/s1678-9946201961008; https://doaj.org/article/73fc20d045494b948f21144597ddc314

ABSTRACT Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.