Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt
Toxoplasma gondii is a parasite with a special predilection for the central nervous system. Toxoplasmosis's contribution to the triggering of many neurodevelopmental disorders was established. This study aimed to detect the seroprevalence and genotypes of T. gondii strains in children with neur...
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ftdoajarticles:oai:doaj.org/article:73eb693824d149cb963ccfc04e0d49a3 2024-09-09T19:26:15+00:00 Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt Sara M. Elzeky Nairmen Nabih Aida A. Abdel-Magied Dina S. Abdelmagid Aya E. Handoussa Marwa M. Hamouda 2022-01-01T00:00:00Z https://doi.org/10.1155/2022/2343679 https://doaj.org/article/73eb693824d149cb963ccfc04e0d49a3 EN eng Wiley http://dx.doi.org/10.1155/2022/2343679 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2022/2343679 https://doaj.org/article/73eb693824d149cb963ccfc04e0d49a3 Journal of Tropical Medicine, Vol 2022 (2022) Arctic medicine. Tropical medicine RC955-962 article 2022 ftdoajarticles https://doi.org/10.1155/2022/2343679 2024-08-05T17:48:41Z Toxoplasma gondii is a parasite with a special predilection for the central nervous system. Toxoplasmosis's contribution to the triggering of many neurodevelopmental disorders was established. This study aimed to detect the seroprevalence and genotypes of T. gondii strains in children with neurodevelopmental disorders. The study included 180 children with neurodevelopmental disorders and 180 children in the control group. Assessment of seropositivity of Toxoplasma IgM and IgG antibodies in patients and controls was carried out. Genetic characterization of T. gondii was obtained by nested polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique targeting dense granule gene (GRA6). Our results showed that the overall seroprevalence of T. gondii antibodies in the patient and controls was 35.6% and 11.7%, respectively. Nested PCR showed positivity in 11.1% of the patient group for T. gondii DNA. T. gondii seropositivity rate was significantly high in patients with hydrocephalus and also in patients with epilepsy. Positive nested PCR was significantly high in children with hydrocephalus only. Genotyping using nested PCR-RFLP showed genotype I (80%) followed by atypical strains (20%) with no association with any specific clinical presentation. In conclusion, among toxoplasmosis-positive children with neurodevelopmental disorders, analysis of T. gondii GRA6 locus revealed the predominance of type I genotype followed by atypical strains. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2022 1 8 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Sara M. Elzeky Nairmen Nabih Aida A. Abdel-Magied Dina S. Abdelmagid Aya E. Handoussa Marwa M. Hamouda Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Toxoplasma gondii is a parasite with a special predilection for the central nervous system. Toxoplasmosis's contribution to the triggering of many neurodevelopmental disorders was established. This study aimed to detect the seroprevalence and genotypes of T. gondii strains in children with neurodevelopmental disorders. The study included 180 children with neurodevelopmental disorders and 180 children in the control group. Assessment of seropositivity of Toxoplasma IgM and IgG antibodies in patients and controls was carried out. Genetic characterization of T. gondii was obtained by nested polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique targeting dense granule gene (GRA6). Our results showed that the overall seroprevalence of T. gondii antibodies in the patient and controls was 35.6% and 11.7%, respectively. Nested PCR showed positivity in 11.1% of the patient group for T. gondii DNA. T. gondii seropositivity rate was significantly high in patients with hydrocephalus and also in patients with epilepsy. Positive nested PCR was significantly high in children with hydrocephalus only. Genotyping using nested PCR-RFLP showed genotype I (80%) followed by atypical strains (20%) with no association with any specific clinical presentation. In conclusion, among toxoplasmosis-positive children with neurodevelopmental disorders, analysis of T. gondii GRA6 locus revealed the predominance of type I genotype followed by atypical strains. |
format |
Article in Journal/Newspaper |
author |
Sara M. Elzeky Nairmen Nabih Aida A. Abdel-Magied Dina S. Abdelmagid Aya E. Handoussa Marwa M. Hamouda |
author_facet |
Sara M. Elzeky Nairmen Nabih Aida A. Abdel-Magied Dina S. Abdelmagid Aya E. Handoussa Marwa M. Hamouda |
author_sort |
Sara M. Elzeky |
title |
Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt |
title_short |
Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt |
title_full |
Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt |
title_fullStr |
Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt |
title_full_unstemmed |
Seroprevalence and Genetic Characterization of Toxoplasma gondii among Children with Neurodevelopmental Disorders in Egypt |
title_sort |
seroprevalence and genetic characterization of toxoplasma gondii among children with neurodevelopmental disorders in egypt |
publisher |
Wiley |
publishDate |
2022 |
url |
https://doi.org/10.1155/2022/2343679 https://doaj.org/article/73eb693824d149cb963ccfc04e0d49a3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2022 (2022) |
op_relation |
http://dx.doi.org/10.1155/2022/2343679 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2022/2343679 https://doaj.org/article/73eb693824d149cb963ccfc04e0d49a3 |
op_doi |
https://doi.org/10.1155/2022/2343679 |
container_title |
Journal of Tropical Medicine |
container_volume |
2022 |
container_start_page |
1 |
op_container_end_page |
8 |
_version_ |
1809895913025437696 |