Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019
Abstract Background Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens their usefulness as malar...
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ftdoajarticles:oai:doaj.org/article:73ea0893e04f4320b049bd40427fb702 2023-05-15T15:17:49+02:00 Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 Agaba B. Bosco Karen Anderson Karryn Gresty Christiane Prosser David Smith Joaniter I. Nankabirwa Sam Nsobya Adoke Yeka Jimmy Opigo Samuel Gonahasa Rhoda Namubiru Emmanuel Arinaitwe Paul Mbaka John Kissa Sungho Won Bora Lee Chae Seung Lim Charles Karamagi Jane Cunningham Joan K. Nakayaga Moses R. Kamya Qin Cheng 2020-08-01T00:00:00Z https://doi.org/10.1186/s12936-020-03362-x https://doaj.org/article/73ea0893e04f4320b049bd40427fb702 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03362-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03362-x 1475-2875 https://doaj.org/article/73ea0893e04f4320b049bd40427fb702 Malaria Journal, Vol 19, Iss 1, Pp 1-14 (2020) Malaria rapid diagnostic tests Plasmodium falciparum Histidine rich protein 2 Histidine rich protein 3 Gene deletion Deoxyribonucleic acid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03362-x 2022-12-31T01:18:04Z Abstract Background Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens their usefulness as malaria diagnostic and surveillance tools. The pfhrp2 and pfhrp3 gene deletions surveillance was conducted in P. falciparum parasite populations in Uganda. Methods Three-hundred (n = 300) P. falciparum isolates collected from cross-sectional malaria surveys in symptomatic individuals in 48 districts of eastern and western Uganda were analysed for the presence of pfhrp2 and pfhrp3 genes. Presence of parasite DNA was confirmed by PCR amplification of the 18s rRNA gene, msp1 and msp2 single copy genes. Presence or absence of deletions was confirmed by amplification of exon1 and exon2 of pfhrp2 and pfhrp3 using gene specific PCR. Results Overall, pfhrp2 and pfhrp3 gene deletions were detected in 29/300 (9.7%, 95% CI 6.6–13.6%) parasite isolates. The pfhrp2 gene was deleted in 10/300 (3.3%, 95% CI 1.6–6.0%) isolates, pfhrp3 in 9/300 (3.0%, 95% CI 1.4–5.6%) while both pfhrp2 and pfhrp3 were deleted in 10/300 (3.3%, 95% CI 1.6–6.0%) parasite isolates. Proportion of pfhrp2/3 deletions was higher in the eastern 14.7% (95% CI 9.7–20.0%) compared to the western region 3.1% (95% CI 0.8–7.7%), p = 0.001. Geographical location was associated with gene deletions aOR 6.25 (2.02–23.55), p = 0.003. Conclusions This is the first large-scale survey reporting the presence of pfhrp2/3 gene deletions in P. falciparum isolates in Uganda. Roll out of RDTs for malaria diagnosis should take into consideration the existence of pfhrp2/3 gene deletions particularly in areas where they were detected. Periodic pfhrp2/3 surveys are recommended to inform future decisions for deployment of alternative RDTs. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria rapid diagnostic tests Plasmodium falciparum Histidine rich protein 2 Histidine rich protein 3 Gene deletion Deoxyribonucleic acid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria rapid diagnostic tests Plasmodium falciparum Histidine rich protein 2 Histidine rich protein 3 Gene deletion Deoxyribonucleic acid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Agaba B. Bosco Karen Anderson Karryn Gresty Christiane Prosser David Smith Joaniter I. Nankabirwa Sam Nsobya Adoke Yeka Jimmy Opigo Samuel Gonahasa Rhoda Namubiru Emmanuel Arinaitwe Paul Mbaka John Kissa Sungho Won Bora Lee Chae Seung Lim Charles Karamagi Jane Cunningham Joan K. Nakayaga Moses R. Kamya Qin Cheng Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 |
topic_facet |
Malaria rapid diagnostic tests Plasmodium falciparum Histidine rich protein 2 Histidine rich protein 3 Gene deletion Deoxyribonucleic acid Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens their usefulness as malaria diagnostic and surveillance tools. The pfhrp2 and pfhrp3 gene deletions surveillance was conducted in P. falciparum parasite populations in Uganda. Methods Three-hundred (n = 300) P. falciparum isolates collected from cross-sectional malaria surveys in symptomatic individuals in 48 districts of eastern and western Uganda were analysed for the presence of pfhrp2 and pfhrp3 genes. Presence of parasite DNA was confirmed by PCR amplification of the 18s rRNA gene, msp1 and msp2 single copy genes. Presence or absence of deletions was confirmed by amplification of exon1 and exon2 of pfhrp2 and pfhrp3 using gene specific PCR. Results Overall, pfhrp2 and pfhrp3 gene deletions were detected in 29/300 (9.7%, 95% CI 6.6–13.6%) parasite isolates. The pfhrp2 gene was deleted in 10/300 (3.3%, 95% CI 1.6–6.0%) isolates, pfhrp3 in 9/300 (3.0%, 95% CI 1.4–5.6%) while both pfhrp2 and pfhrp3 were deleted in 10/300 (3.3%, 95% CI 1.6–6.0%) parasite isolates. Proportion of pfhrp2/3 deletions was higher in the eastern 14.7% (95% CI 9.7–20.0%) compared to the western region 3.1% (95% CI 0.8–7.7%), p = 0.001. Geographical location was associated with gene deletions aOR 6.25 (2.02–23.55), p = 0.003. Conclusions This is the first large-scale survey reporting the presence of pfhrp2/3 gene deletions in P. falciparum isolates in Uganda. Roll out of RDTs for malaria diagnosis should take into consideration the existence of pfhrp2/3 gene deletions particularly in areas where they were detected. Periodic pfhrp2/3 surveys are recommended to inform future decisions for deployment of alternative RDTs. |
format |
Article in Journal/Newspaper |
author |
Agaba B. Bosco Karen Anderson Karryn Gresty Christiane Prosser David Smith Joaniter I. Nankabirwa Sam Nsobya Adoke Yeka Jimmy Opigo Samuel Gonahasa Rhoda Namubiru Emmanuel Arinaitwe Paul Mbaka John Kissa Sungho Won Bora Lee Chae Seung Lim Charles Karamagi Jane Cunningham Joan K. Nakayaga Moses R. Kamya Qin Cheng |
author_facet |
Agaba B. Bosco Karen Anderson Karryn Gresty Christiane Prosser David Smith Joaniter I. Nankabirwa Sam Nsobya Adoke Yeka Jimmy Opigo Samuel Gonahasa Rhoda Namubiru Emmanuel Arinaitwe Paul Mbaka John Kissa Sungho Won Bora Lee Chae Seung Lim Charles Karamagi Jane Cunningham Joan K. Nakayaga Moses R. Kamya Qin Cheng |
author_sort |
Agaba B. Bosco |
title |
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 |
title_short |
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 |
title_full |
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 |
title_fullStr |
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 |
title_full_unstemmed |
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019 |
title_sort |
molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in plasmodium falciparum parasite populations in uganda, 2017–2019 |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03362-x https://doaj.org/article/73ea0893e04f4320b049bd40427fb702 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-14 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03362-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03362-x 1475-2875 https://doaj.org/article/73ea0893e04f4320b049bd40427fb702 |
op_doi |
https://doi.org/10.1186/s12936-020-03362-x |
container_title |
Malaria Journal |
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19 |
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1766348087525113856 |