Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter
A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs. Twenty-seven adult dogs were enrolled and fed a diet based on a mixture of rice and pasta with fresh raw meat (CD). After 90 d, 8 dogs continue...
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ftdoajarticles:oai:doaj.org/article:73e60df6224e4e52803951181493e31d 2023-05-15T15:51:09+02:00 Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter Misa Sandri Sandy Sgorlon Elisa Scarsella Bruno Stefanon 2020-09-01T00:00:00Z https://doi.org/10.1016/j.aninu.2020.03.003 https://doaj.org/article/73e60df6224e4e52803951181493e31d EN eng KeAi Communications Co., Ltd. http://www.sciencedirect.com/science/article/pii/S2405654520300366 https://doaj.org/toc/2405-6545 2405-6545 doi:10.1016/j.aninu.2020.03.003 https://doaj.org/article/73e60df6224e4e52803951181493e31d Animal Nutrition, Vol 6, Iss 3, Pp 353-361 (2020) Diets Starch fraction Microbiome Nutrition Canis lupus familiaris Animal culture SF1-1100 article 2020 ftdoajarticles https://doi.org/10.1016/j.aninu.2020.03.003 2022-12-31T05:30:52Z A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs. Twenty-seven adult dogs were enrolled and fed a diet based on a mixture of rice and pasta with fresh raw meat (CD). After 90 d, 8 dogs continued to receive CD diet, 10 dogs received a diet made of a raw meat and a complementary food with rice as the main source of starch (B1), and 9 dogs were fed a diet with the same raw meat and a complementary food with potato as the main source of starch (B2). Samples of feces were collected from each dog in the mornings at the beginning of the study and after 15 d and analyzed for pH, ammonia N (N–NH3) and total N, short chain fatty acids (SCFA) and lactic acid. Relative abundance of fecal microbiota was assessed by sequencing and annotating the V3–V4 regions of the 16S rRNA. Total starch intake was similar between diets but differed in the in vitro rate digestion and in the resistant starch, which was higher in B2 than in B1 and CD diets. Dogs fed B2 diet showed lower (P < 0.05) N–NH3 and pH but higher (P < 0.05) molar proportion of lactic acid. Linear discriminant analysis of the genera relative abundances indicated a significant (P < 0.01) increase of SMB53 genus at the end of the study in B1 diet and of Megamonas genus in B1 and B2 diets in comparison to CD diet. These results suggest that changes of starch source in a raw meat-based diet have limited effects on fecal microbiome in healthy dogs, but underline a high variability of microbiota among dogs. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Animal Nutrition 6 3 353 361 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Diets Starch fraction Microbiome Nutrition Canis lupus familiaris Animal culture SF1-1100 |
spellingShingle |
Diets Starch fraction Microbiome Nutrition Canis lupus familiaris Animal culture SF1-1100 Misa Sandri Sandy Sgorlon Elisa Scarsella Bruno Stefanon Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
topic_facet |
Diets Starch fraction Microbiome Nutrition Canis lupus familiaris Animal culture SF1-1100 |
description |
A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs. Twenty-seven adult dogs were enrolled and fed a diet based on a mixture of rice and pasta with fresh raw meat (CD). After 90 d, 8 dogs continued to receive CD diet, 10 dogs received a diet made of a raw meat and a complementary food with rice as the main source of starch (B1), and 9 dogs were fed a diet with the same raw meat and a complementary food with potato as the main source of starch (B2). Samples of feces were collected from each dog in the mornings at the beginning of the study and after 15 d and analyzed for pH, ammonia N (N–NH3) and total N, short chain fatty acids (SCFA) and lactic acid. Relative abundance of fecal microbiota was assessed by sequencing and annotating the V3–V4 regions of the 16S rRNA. Total starch intake was similar between diets but differed in the in vitro rate digestion and in the resistant starch, which was higher in B2 than in B1 and CD diets. Dogs fed B2 diet showed lower (P < 0.05) N–NH3 and pH but higher (P < 0.05) molar proportion of lactic acid. Linear discriminant analysis of the genera relative abundances indicated a significant (P < 0.01) increase of SMB53 genus at the end of the study in B1 diet and of Megamonas genus in B1 and B2 diets in comparison to CD diet. These results suggest that changes of starch source in a raw meat-based diet have limited effects on fecal microbiome in healthy dogs, but underline a high variability of microbiota among dogs. |
format |
Article in Journal/Newspaper |
author |
Misa Sandri Sandy Sgorlon Elisa Scarsella Bruno Stefanon |
author_facet |
Misa Sandri Sandy Sgorlon Elisa Scarsella Bruno Stefanon |
author_sort |
Misa Sandri |
title |
Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
title_short |
Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
title_full |
Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
title_fullStr |
Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
title_full_unstemmed |
Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
title_sort |
effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter |
publisher |
KeAi Communications Co., Ltd. |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.aninu.2020.03.003 https://doaj.org/article/73e60df6224e4e52803951181493e31d |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Animal Nutrition, Vol 6, Iss 3, Pp 353-361 (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2405654520300366 https://doaj.org/toc/2405-6545 2405-6545 doi:10.1016/j.aninu.2020.03.003 https://doaj.org/article/73e60df6224e4e52803951181493e31d |
op_doi |
https://doi.org/10.1016/j.aninu.2020.03.003 |
container_title |
Animal Nutrition |
container_volume |
6 |
container_issue |
3 |
container_start_page |
353 |
op_container_end_page |
361 |
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1766386214424805376 |