Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético
Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast ce...
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ftdoajarticles:oai:doaj.org/article:73a3a01113174646a1041eb18ecc1047 2024-09-09T19:28:06+00:00 Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético Cilmery S. Kurokawa Catalina R. Lopes Maria F. Sugizaki Eiko E. Kuramae Marcello F. Franco Maria T. S. Peraçoli 2005-10-01T00:00:00Z https://doi.org/10.1590/S0036-46652005000500004 https://doaj.org/article/73a3a01113174646a1041eb18ecc1047 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000500004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652005000500004 0036-4665 1678-9946 https://doaj.org/article/73a3a01113174646a1041eb18ecc1047 Revista do Instituto de Medicina Tropical de São Paulo, Vol 47, Iss 5, Pp 257-262 (2005) Antibodies Colony morphology Paracoccidioides brasiliensis RAPD Virulence Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2005 ftdoajarticles https://doi.org/10.1590/S0036-46652005000500004 2024-08-05T17:49:30Z Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast cell recovery from lungs and demonstration of histopathologic lesions in different organs. The results showed that the isolates presented four virulence degrees: high virulence, intermediate, low and non-virulence. RAPD clustered the isolates studied in two main groups with 56% of genetic similarity. Strains with low virulence, Pb265 or the non-virulent, Pb192, showed glabrous/cerebriform morphology and high genetic similarity (98.7%) when compared to the other isolates studied. The same was observed with Bt79 and Bt83 that shared 96% genetic similarity, cottony colonies and high virulence. The RAPD technique could only discriminate P. brasiliensis isolates according to glabrous/cerebriform or cottony colonies, and also high from low virulence strains. Isolates with intermediate virulence such as Pb18, Pb18B6, Bt32 and Bt56 showed variability in their similarity coefficient suggesting that RAPD was able to detect genetic variability in this fungal specie. Virulence profile of P. brasiliensis demonstrated that both mycelial morphologic extreme phenotypes may be associated with fungal virulence and their in vitro subculture time. Thus, RAPD technique analysis employed in association with virulence, morphologic and immunologic aspects might prove adequate to detect differences between P. brasiliensis isolates. Dez isolados de P. brasiliensis foram avaliados em relação à patogenicidade por inoculação intravenosa em camundongos e associação com morfologia miceliana e padrão genético por amplificação genônica do DNA polimórfico (RAPD). A patogenicidade, avaliada por recuperação de fungos viáveis a partir de tecido pulmonar e por lesões histopatológicas em diferentes órgãos, mostrou que os isolados apresentaram quatro graus de ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 47 5 257 262 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Antibodies Colony morphology Paracoccidioides brasiliensis RAPD Virulence Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Antibodies Colony morphology Paracoccidioides brasiliensis RAPD Virulence Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Cilmery S. Kurokawa Catalina R. Lopes Maria F. Sugizaki Eiko E. Kuramae Marcello F. Franco Maria T. S. Peraçoli Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético |
topic_facet |
Antibodies Colony morphology Paracoccidioides brasiliensis RAPD Virulence Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast cell recovery from lungs and demonstration of histopathologic lesions in different organs. The results showed that the isolates presented four virulence degrees: high virulence, intermediate, low and non-virulence. RAPD clustered the isolates studied in two main groups with 56% of genetic similarity. Strains with low virulence, Pb265 or the non-virulent, Pb192, showed glabrous/cerebriform morphology and high genetic similarity (98.7%) when compared to the other isolates studied. The same was observed with Bt79 and Bt83 that shared 96% genetic similarity, cottony colonies and high virulence. The RAPD technique could only discriminate P. brasiliensis isolates according to glabrous/cerebriform or cottony colonies, and also high from low virulence strains. Isolates with intermediate virulence such as Pb18, Pb18B6, Bt32 and Bt56 showed variability in their similarity coefficient suggesting that RAPD was able to detect genetic variability in this fungal specie. Virulence profile of P. brasiliensis demonstrated that both mycelial morphologic extreme phenotypes may be associated with fungal virulence and their in vitro subculture time. Thus, RAPD technique analysis employed in association with virulence, morphologic and immunologic aspects might prove adequate to detect differences between P. brasiliensis isolates. Dez isolados de P. brasiliensis foram avaliados em relação à patogenicidade por inoculação intravenosa em camundongos e associação com morfologia miceliana e padrão genético por amplificação genônica do DNA polimórfico (RAPD). A patogenicidade, avaliada por recuperação de fungos viáveis a partir de tecido pulmonar e por lesões histopatológicas em diferentes órgãos, mostrou que os isolados apresentaram quatro graus de ... |
format |
Article in Journal/Newspaper |
author |
Cilmery S. Kurokawa Catalina R. Lopes Maria F. Sugizaki Eiko E. Kuramae Marcello F. Franco Maria T. S. Peraçoli |
author_facet |
Cilmery S. Kurokawa Catalina R. Lopes Maria F. Sugizaki Eiko E. Kuramae Marcello F. Franco Maria T. S. Peraçoli |
author_sort |
Cilmery S. Kurokawa |
title |
Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético |
title_short |
Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético |
title_full |
Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético |
title_fullStr |
Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético |
title_full_unstemmed |
Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético |
title_sort |
virulence profile of ten paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns perfil de virulência de dez isolados de paracoccidioides brasiliensis: associação com morfologia e padrão genético |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2005 |
url |
https://doi.org/10.1590/S0036-46652005000500004 https://doaj.org/article/73a3a01113174646a1041eb18ecc1047 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 47, Iss 5, Pp 257-262 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000500004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652005000500004 0036-4665 1678-9946 https://doaj.org/article/73a3a01113174646a1041eb18ecc1047 |
op_doi |
https://doi.org/10.1590/S0036-46652005000500004 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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47 |
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257 |
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