Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities
Lichens have developed numerous adaptations to optimise their survival under harsh abiotic stress, colonise different substrates, and reach substantial population sizes and high coverage in ice-free Antarctic areas, benefiting from a symbiotic lifestyle. As lichen thalli represent consortia with an...
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ftdoajarticles:oai:doaj.org/article:7393c159b0944fff89f5b2b07b414631 2023-06-11T04:06:06+02:00 Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities Andreas Beck Angélica Casanova-Katny Julia Gerasimova 2023-04-01T00:00:00Z https://doi.org/10.3390/genes14051019 https://doaj.org/article/7393c159b0944fff89f5b2b07b414631 EN eng MDPI AG https://www.mdpi.com/2073-4425/14/5/1019 https://doaj.org/toc/2073-4425 doi:10.3390/genes14051019 2073-4425 https://doaj.org/article/7393c159b0944fff89f5b2b07b414631 Genes, Vol 14, Iss 1019, p 1019 (2023) Himantormia Placopsis Ramalina lichen-associated eukaryotes deglaciation time Genetics QH426-470 article 2023 ftdoajarticles https://doi.org/10.3390/genes14051019 2023-05-28T00:34:03Z Lichens have developed numerous adaptations to optimise their survival under harsh abiotic stress, colonise different substrates, and reach substantial population sizes and high coverage in ice-free Antarctic areas, benefiting from a symbiotic lifestyle. As lichen thalli represent consortia with an unknown number of participants, it is important to know about the accessory organisms and their relationships with various environmental conditions. To this end, we analysed lichen-associated communities from Himantormia lugubris , Placopsis antarctica , P. contortuplicata , and Ramalina terebrata , collected from soils with differing deglaciation times, using a metabarcoding approach. In general, many more Ascomycete taxa are associated with the investigated lichens compared to Basidiomycota. Given our sampling, a consistently higher number of lichen-associated eukaryotes are estimated to be present in areas with deglaciation times of longer than 5000 years compared to more recently deglaciated areas. Thus far, members of Dothideomycetes, Leotiomycetes, and Arthoniomycetes have been restricted to the Placopsis specimens from areas with deglaciation times longer than 5000 years. Striking differences between the associated organisms of R. terebrata and H. lugubris have also been discovered. Thus, a species-specific basidiomycete, Tremella , was revealed for R. terebrata , as was a member of Capnodiales for H. lugubris . Our study provides further understanding of the complex terricolous lichen-associated mycobiome using the metabarcoding approach. It also illustrates the necessity to extend our knowledge of complex lichen symbiosis and further improve the coverage of microbial eukaryotes in DNA barcode libraries, including more extended sampling. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Genes 14 5 1019 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Himantormia Placopsis Ramalina lichen-associated eukaryotes deglaciation time Genetics QH426-470 |
spellingShingle |
Himantormia Placopsis Ramalina lichen-associated eukaryotes deglaciation time Genetics QH426-470 Andreas Beck Angélica Casanova-Katny Julia Gerasimova Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities |
topic_facet |
Himantormia Placopsis Ramalina lichen-associated eukaryotes deglaciation time Genetics QH426-470 |
description |
Lichens have developed numerous adaptations to optimise their survival under harsh abiotic stress, colonise different substrates, and reach substantial population sizes and high coverage in ice-free Antarctic areas, benefiting from a symbiotic lifestyle. As lichen thalli represent consortia with an unknown number of participants, it is important to know about the accessory organisms and their relationships with various environmental conditions. To this end, we analysed lichen-associated communities from Himantormia lugubris , Placopsis antarctica , P. contortuplicata , and Ramalina terebrata , collected from soils with differing deglaciation times, using a metabarcoding approach. In general, many more Ascomycete taxa are associated with the investigated lichens compared to Basidiomycota. Given our sampling, a consistently higher number of lichen-associated eukaryotes are estimated to be present in areas with deglaciation times of longer than 5000 years compared to more recently deglaciated areas. Thus far, members of Dothideomycetes, Leotiomycetes, and Arthoniomycetes have been restricted to the Placopsis specimens from areas with deglaciation times longer than 5000 years. Striking differences between the associated organisms of R. terebrata and H. lugubris have also been discovered. Thus, a species-specific basidiomycete, Tremella , was revealed for R. terebrata , as was a member of Capnodiales for H. lugubris . Our study provides further understanding of the complex terricolous lichen-associated mycobiome using the metabarcoding approach. It also illustrates the necessity to extend our knowledge of complex lichen symbiosis and further improve the coverage of microbial eukaryotes in DNA barcode libraries, including more extended sampling. |
format |
Article in Journal/Newspaper |
author |
Andreas Beck Angélica Casanova-Katny Julia Gerasimova |
author_facet |
Andreas Beck Angélica Casanova-Katny Julia Gerasimova |
author_sort |
Andreas Beck |
title |
Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities |
title_short |
Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities |
title_full |
Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities |
title_fullStr |
Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities |
title_full_unstemmed |
Metabarcoding of Antarctic Lichens from Areas with Different Deglaciation Times Reveals a High Diversity of Lichen-Associated Communities |
title_sort |
metabarcoding of antarctic lichens from areas with different deglaciation times reveals a high diversity of lichen-associated communities |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/genes14051019 https://doaj.org/article/7393c159b0944fff89f5b2b07b414631 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Genes, Vol 14, Iss 1019, p 1019 (2023) |
op_relation |
https://www.mdpi.com/2073-4425/14/5/1019 https://doaj.org/toc/2073-4425 doi:10.3390/genes14051019 2073-4425 https://doaj.org/article/7393c159b0944fff89f5b2b07b414631 |
op_doi |
https://doi.org/10.3390/genes14051019 |
container_title |
Genes |
container_volume |
14 |
container_issue |
5 |
container_start_page |
1019 |
_version_ |
1768377883444641792 |