Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice
Abstract Background Induction of potent long lasting effector T cell responses against liver stage malaria antigens strongly correlates with protection from malaria. While Adenovirus serotype 5 (Ad5) based malaria vaccine platforms have the ability to induce potent effector T cell responses against...
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ftdoajarticles:oai:doaj.org/article:737c77a1cce245169e5952e9e1c5a623 2023-05-15T15:17:39+02:00 Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice Schuldt Nathaniel J Aldhamen Yasser A Godbehere-Roosa Sarah Seregin Sergey S Kousa Youssef A Amalfitano Andrea 2012-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-209 https://doaj.org/article/737c77a1cce245169e5952e9e1c5a623 EN eng BMC http://www.malariajournal.com/content/11/1/209 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-209 1475-2875 https://doaj.org/article/737c77a1cce245169e5952e9e1c5a623 Malaria Journal, Vol 11, Iss 1, p 209 (2012) Serotype 5 Serotype 4 Adenovirus Malaria Circumsporozoite protein Vaccine Heterologous Homologous Prime Boost Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-209 2022-12-31T04:52:28Z Abstract Background Induction of potent long lasting effector T cell responses against liver stage malaria antigens strongly correlates with protection from malaria. While Adenovirus serotype 5 (Ad5) based malaria vaccine platforms have the ability to induce potent effector T cell responses against transgenes, high rates of pre-existing Ad5 immunity in malaria endemic regions has prompted study of alternative Ad serotype based malaria vaccines as replacements for Ad5 based malaria vaccines. The research described in this article examines the utility of alternative serotype adenovirus serotype 4 (Ad4) expressing a sporozoite surface protein (circumsporozoite protein (CSP)) (Ad4-CSP) to induce immune responses against CSP. The immunogenicity of Ad4-CSP was also tested in homologous and heterologous prime boost vaccinations in both Ad5 naïve and Ad5 immune backgrounds as compared to use of Ad5-CSP. Results In Ad5 naïve animals, use of Ad4-CSP priming vaccinations followed by boosting with Ad5-CSP (Ad4-CSP/Ad5-CSP) maximally increased the numbers of CSP specific cytokine secreting cytotoxic T cells relative to repeated use of Ad5-CSP. The Ad4-CSP/Ad5-CSP regimen also induced equivalent levels of CSP specific cell killing as did homologous prime-boost vaccinations with Ad5-CSP, despite stimulating lower numbers of CSP specific cytotoxic T cells. Priming with Ad4-CSP followed by a homologous boost resulted in significantly less CSP specific humoral responses than any other vaccination regimen tested in Ad naïve animals. In Ad5 immune animals, addition of Ad4-CSP in homologous or heterologous prime boost resulted in inductions of higher CSP specific responses than animals repeatedly vaccinated with Ad5-CSP alone. However, the observed responses were well below those observed in similarly treated Ad naïve mice. Conclusions While the Ad4-CSP/Ad5-CSP and Ad5-CSP/Ad5-CSP vaccination regimens resulted in equivalent CSP specific killing in Ad naïve animals, Ad4-CSP/Ad5-CSP achieved this result with a lower percentage of CSP ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 209 |
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Serotype 5 Serotype 4 Adenovirus Malaria Circumsporozoite protein Vaccine Heterologous Homologous Prime Boost Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Serotype 5 Serotype 4 Adenovirus Malaria Circumsporozoite protein Vaccine Heterologous Homologous Prime Boost Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Schuldt Nathaniel J Aldhamen Yasser A Godbehere-Roosa Sarah Seregin Sergey S Kousa Youssef A Amalfitano Andrea Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice |
topic_facet |
Serotype 5 Serotype 4 Adenovirus Malaria Circumsporozoite protein Vaccine Heterologous Homologous Prime Boost Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Induction of potent long lasting effector T cell responses against liver stage malaria antigens strongly correlates with protection from malaria. While Adenovirus serotype 5 (Ad5) based malaria vaccine platforms have the ability to induce potent effector T cell responses against transgenes, high rates of pre-existing Ad5 immunity in malaria endemic regions has prompted study of alternative Ad serotype based malaria vaccines as replacements for Ad5 based malaria vaccines. The research described in this article examines the utility of alternative serotype adenovirus serotype 4 (Ad4) expressing a sporozoite surface protein (circumsporozoite protein (CSP)) (Ad4-CSP) to induce immune responses against CSP. The immunogenicity of Ad4-CSP was also tested in homologous and heterologous prime boost vaccinations in both Ad5 naïve and Ad5 immune backgrounds as compared to use of Ad5-CSP. Results In Ad5 naïve animals, use of Ad4-CSP priming vaccinations followed by boosting with Ad5-CSP (Ad4-CSP/Ad5-CSP) maximally increased the numbers of CSP specific cytokine secreting cytotoxic T cells relative to repeated use of Ad5-CSP. The Ad4-CSP/Ad5-CSP regimen also induced equivalent levels of CSP specific cell killing as did homologous prime-boost vaccinations with Ad5-CSP, despite stimulating lower numbers of CSP specific cytotoxic T cells. Priming with Ad4-CSP followed by a homologous boost resulted in significantly less CSP specific humoral responses than any other vaccination regimen tested in Ad naïve animals. In Ad5 immune animals, addition of Ad4-CSP in homologous or heterologous prime boost resulted in inductions of higher CSP specific responses than animals repeatedly vaccinated with Ad5-CSP alone. However, the observed responses were well below those observed in similarly treated Ad naïve mice. Conclusions While the Ad4-CSP/Ad5-CSP and Ad5-CSP/Ad5-CSP vaccination regimens resulted in equivalent CSP specific killing in Ad naïve animals, Ad4-CSP/Ad5-CSP achieved this result with a lower percentage of CSP ... |
format |
Article in Journal/Newspaper |
author |
Schuldt Nathaniel J Aldhamen Yasser A Godbehere-Roosa Sarah Seregin Sergey S Kousa Youssef A Amalfitano Andrea |
author_facet |
Schuldt Nathaniel J Aldhamen Yasser A Godbehere-Roosa Sarah Seregin Sergey S Kousa Youssef A Amalfitano Andrea |
author_sort |
Schuldt Nathaniel J |
title |
Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice |
title_short |
Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice |
title_full |
Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice |
title_fullStr |
Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice |
title_full_unstemmed |
Immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and Adenovirus (Ad5) immune mice |
title_sort |
immunogenicity when utilizing adenovirus serotype 4 and 5 vaccines expressing circumsporozoite protein in naïve and adenovirus (ad5) immune mice |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-209 https://doaj.org/article/737c77a1cce245169e5952e9e1c5a623 |
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Arctic |
geographic_facet |
Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 209 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/209 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-209 1475-2875 https://doaj.org/article/737c77a1cce245169e5952e9e1c5a623 |
op_doi |
https://doi.org/10.1186/1475-2875-11-209 |
container_title |
Malaria Journal |
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11 |
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209 |
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1766347902344495104 |