Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer
Abstract Background The reindeer ( Rangifer tarandus tarandus ) industry in Alaska began with animals imported from Siberia (Russia) in the 1890's. Cervid herpes virus 2 (CvHV2) is endemic in reindeer in Scandinavia. We sought to determine if the same virus, or similar herpesviruses, were circu...
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ftdoajarticles:oai:doaj.org/article:737715307f754518a33729523287fdb7 2023-05-15T15:14:26+02:00 Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer Evans Alina L das Neves Carlos G Finstad Greg F Beckmen Kimberlee B Skjerve Eystein Nymo Ingebjørg H Tryland Morten 2012-01-01T00:00:00Z https://doi.org/10.1186/1746-6148-8-5 https://doaj.org/article/737715307f754518a33729523287fdb7 EN eng BMC http://www.biomedcentral.com/1746-6148/8/5 https://doaj.org/toc/1746-6148 doi:10.1186/1746-6148-8-5 1746-6148 https://doaj.org/article/737715307f754518a33729523287fdb7 BMC Veterinary Research, Vol 8, Iss 1, p 5 (2012) caribou epidemiology herd health herpesvirus infectious diseases reindeer Rangifer wildlife medicine Veterinary medicine SF600-1100 article 2012 ftdoajarticles https://doi.org/10.1186/1746-6148-8-5 2022-12-31T01:30:21Z Abstract Background The reindeer ( Rangifer tarandus tarandus ) industry in Alaska began with animals imported from Siberia (Russia) in the 1890's. Cervid herpes virus 2 (CvHV2) is endemic in reindeer in Scandinavia. We sought to determine if the same virus, or similar herpesviruses, were circulating in Alaskan reindeer and caribou ( Rangifer tarandus granti ). Serum samples from 292 reindeer were collected during annual reindeer handlings (1988-2005) near Nome, Alaska. In 2005, swab samples were collected from 40 calves from this herd, near Nome, Alaska. In 2007, ocular and nasal swab samples were collected from 30 apparently healthy reindeer calves near Wales, Alaska. Samples of plasma and white blood cells were collected from three Alaskan caribou herds, Mulchatna (n = 24), Teshekpuk (n = 34) and the Western Arctic (n = 87) in 2009. Results Of 292 reindeer samples tested by ELISA for antibodies against alphaherpesvirus (bovine herpesvirus 1 as antigen), seroprevalence was 47% (136/292) and adult reindeer had higher seroprevalence than yearlings. The overall seroprevalence for caribou was 60% (87/145), with no significant differences among caribou herds. A virus neutralization test of 20 samples from both reindeer and caribou showed that ELISA positive samples always neutralized CvHV2 to a greater extent than BoHV1 or elk herpesvirus (ElkHV), indicating that CvHv2 is the most likely virus circulating. PCR of nasal and ocular swabs sampled from 30 reindeer calves in Wales, Alaska (2007) yielded four CvHV2 positive samples. PCR amplicons of the expected size (294 bp) were obtained from 2 of the 36 buffy coats samples from caribou, and the amplicon sequences were consistent with CvHV2. Conclusions This study shows that Alaskan reindeer and Caribou are infected with an alphaherpesvirus. Based on sequence similarity, CvHV-2 is the most likely virus. Further studies should be conducted to determine the impact of this infection on the health of these animals. Article in Journal/Newspaper Arctic elk Nome Rangifer tarandus Alaska Siberia Directory of Open Access Journals: DOAJ Articles Arctic BMC Veterinary Research 8 1 5 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
caribou epidemiology herd health herpesvirus infectious diseases reindeer Rangifer wildlife medicine Veterinary medicine SF600-1100 |
spellingShingle |
caribou epidemiology herd health herpesvirus infectious diseases reindeer Rangifer wildlife medicine Veterinary medicine SF600-1100 Evans Alina L das Neves Carlos G Finstad Greg F Beckmen Kimberlee B Skjerve Eystein Nymo Ingebjørg H Tryland Morten Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer |
topic_facet |
caribou epidemiology herd health herpesvirus infectious diseases reindeer Rangifer wildlife medicine Veterinary medicine SF600-1100 |
description |
Abstract Background The reindeer ( Rangifer tarandus tarandus ) industry in Alaska began with animals imported from Siberia (Russia) in the 1890's. Cervid herpes virus 2 (CvHV2) is endemic in reindeer in Scandinavia. We sought to determine if the same virus, or similar herpesviruses, were circulating in Alaskan reindeer and caribou ( Rangifer tarandus granti ). Serum samples from 292 reindeer were collected during annual reindeer handlings (1988-2005) near Nome, Alaska. In 2005, swab samples were collected from 40 calves from this herd, near Nome, Alaska. In 2007, ocular and nasal swab samples were collected from 30 apparently healthy reindeer calves near Wales, Alaska. Samples of plasma and white blood cells were collected from three Alaskan caribou herds, Mulchatna (n = 24), Teshekpuk (n = 34) and the Western Arctic (n = 87) in 2009. Results Of 292 reindeer samples tested by ELISA for antibodies against alphaherpesvirus (bovine herpesvirus 1 as antigen), seroprevalence was 47% (136/292) and adult reindeer had higher seroprevalence than yearlings. The overall seroprevalence for caribou was 60% (87/145), with no significant differences among caribou herds. A virus neutralization test of 20 samples from both reindeer and caribou showed that ELISA positive samples always neutralized CvHV2 to a greater extent than BoHV1 or elk herpesvirus (ElkHV), indicating that CvHv2 is the most likely virus circulating. PCR of nasal and ocular swabs sampled from 30 reindeer calves in Wales, Alaska (2007) yielded four CvHV2 positive samples. PCR amplicons of the expected size (294 bp) were obtained from 2 of the 36 buffy coats samples from caribou, and the amplicon sequences were consistent with CvHV2. Conclusions This study shows that Alaskan reindeer and Caribou are infected with an alphaherpesvirus. Based on sequence similarity, CvHV-2 is the most likely virus. Further studies should be conducted to determine the impact of this infection on the health of these animals. |
format |
Article in Journal/Newspaper |
author |
Evans Alina L das Neves Carlos G Finstad Greg F Beckmen Kimberlee B Skjerve Eystein Nymo Ingebjørg H Tryland Morten |
author_facet |
Evans Alina L das Neves Carlos G Finstad Greg F Beckmen Kimberlee B Skjerve Eystein Nymo Ingebjørg H Tryland Morten |
author_sort |
Evans Alina L |
title |
Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer |
title_short |
Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer |
title_full |
Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer |
title_fullStr |
Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer |
title_full_unstemmed |
Evidence of alphaherpesvirus infections in Alaskan caribou and reindeer |
title_sort |
evidence of alphaherpesvirus infections in alaskan caribou and reindeer |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1746-6148-8-5 https://doaj.org/article/737715307f754518a33729523287fdb7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic elk Nome Rangifer tarandus Alaska Siberia |
genre_facet |
Arctic elk Nome Rangifer tarandus Alaska Siberia |
op_source |
BMC Veterinary Research, Vol 8, Iss 1, p 5 (2012) |
op_relation |
http://www.biomedcentral.com/1746-6148/8/5 https://doaj.org/toc/1746-6148 doi:10.1186/1746-6148-8-5 1746-6148 https://doaj.org/article/737715307f754518a33729523287fdb7 |
op_doi |
https://doi.org/10.1186/1746-6148-8-5 |
container_title |
BMC Veterinary Research |
container_volume |
8 |
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1 |
container_start_page |
5 |
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1766344883082100736 |