Proteomic characterization of Naja mandalayensis venom

• Published in: Journal of Venomous Animals and Toxins including Tropical Diseases
• Main Authors: Emídio Beraldo Neto, Guilherme Rabelo Coelho, Juliana Mozer Sciani, Daniel Carvalho Pimenta
• Format: Article in Journal/Newspaper
• Language: English
• Published: SciELO 2021
• Subjects: Naja mandalayensis; Proteome; Spitting cobra; Three-finger toxins; SVMP; Enzymes; Arctic medicine. Tropical medicine; RC955-962; Toxicology. Poisons; RA1190-1270; Zoology; QL1-991; Arctic
• Online Access: https://doi.org/10.1590/1678-9199-jvatitd-2020-0125; https://doaj.org/article/736ce1222d50434e8b8962a5af7c6ebe

Abstract Background Naja mandalayensis is a spitting cobra from Myanmar. To the best of our knowledge, no studies on this venom composition have been conducted so far. On the other hand, few envenomation descriptions state that it elicits mainly local inflammation in the victims’ eyes, the preferred target of this spiting cobra. Symptoms would typically include burning and painful sensation, conjunctivitis, edema and temporary loss of vision. Methods We have performed a liquid-chromatography (C18-RP-HPLC) mass spectrometry (ESI-IT-TOF/MS) based approach in order to biochemically characterize N. mandalayensis venom. Results A wide variety of three-finger toxins (cardiotoxins) and metallopeptidases were detected. Less abundant, but still representative, were cysteine-rich secretory proteins, L-amino-acid oxidases, phospholipases A2, venom 5'-nucleotidase and a serine peptidase inhibitor. Other proteins were present, but were detected in a relatively small concentration. Conclusion The present study set the basis for a better comprehension of the envenomation from a molecular perspective and, by increasing the interest and information available for this species, allows future venom comparisons among cobras and their diverse venom proteins.