Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan
Abstract Background Vivax malaria is a leading public health concern worldwide. Due to the high prevalence of Duffy-negative blood group population, Plasmodium vivax in Africa historically is less attributable and remains a neglected disease. The interaction between Duffy binding protein and its cog...
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ftdoajarticles:oai:doaj.org/article:735ee02cbb824e769f10d65f2c0b770a 2023-05-15T15:17:16+02:00 Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan Mohammad Rafiul Hoque Mohammed Mohieldien Abbas Elfaki Md Atique Ahmed Seong-Kyun Lee Fauzi Muh Musab M. Ali Albsheer Muzamil Mahdi Abdel Hamid Eun-Taek Han 2018-08-01T00:00:00Z https://doi.org/10.1186/s12936-018-2425-z https://doaj.org/article/735ee02cbb824e769f10d65f2c0b770a EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2425-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2425-z 1475-2875 https://doaj.org/article/735ee02cbb824e769f10d65f2c0b770a Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) Malaria Plasmodium vivax Sudan Haplotype Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2425-z 2022-12-31T00:22:33Z Abstract Background Vivax malaria is a leading public health concern worldwide. Due to the high prevalence of Duffy-negative blood group population, Plasmodium vivax in Africa historically is less attributable and remains a neglected disease. The interaction between Duffy binding protein and its cognate receptor, Duffy antigen receptor for chemokine plays a key role in the invasion of red blood cells and serves as a novel vaccine candidate against P. vivax. However, the polymorphic nature of P. vivax Duffy binding protein (DBP), particularly N-terminal cysteine-rich region (PvDBPII), represents a major obstacle for the successful design of a DBP-based vaccine to enable global protection. In this study, the level of pvdbpII sequence variations, Duffy blood group genotypes, number of haplotypes circulating, and the natural selection at pvdbpII in Sudan isolates were analysed and the implication in terms of DBP-based vaccine design was discussed. Methods Forty-two P. vivax-infected blood samples were collected from patients from different areas of Sudan during 2014–2016. For Duffy blood group genotyping, the fragment that indicates GATA-1 transcription factor binding site of the FY gene (− 33T > C) was amplified by PCR and sequenced by direct sequencing. The region II flanking pvdbpII was PCR amplified and sequenced by direct sequencing. The genetic diversity and natural selection of pvdbpII were done using DnaSP ver 5.0 and MEGA ver 5.0 programs. Based on predominant, non-synonymous, single nucleotide polymorphisms (SNPs), prevalence of Sudanese haplotypes was assessed in global isolates. Results Twenty SNPs (14 non-synonymous and 6 synonymous) were identified in pvdbpII among the 42 Sudan P. vivax isolates. Sequence analysis revealed that 11 different PvDBP haplotypes exist in Sudan P. vivax isolates and the region has evolved under positive selection. Among the identified PvDBP haplotypes five PvDBP haplotypes were shared among Duffy-negative as well as Duffy-positive individuals. The high selective pressure ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Gata ENVELOPE(-19.702,-19.702,63.540,63.540) Malaria Journal 17 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Plasmodium vivax Sudan Haplotype Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Plasmodium vivax Sudan Haplotype Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mohammad Rafiul Hoque Mohammed Mohieldien Abbas Elfaki Md Atique Ahmed Seong-Kyun Lee Fauzi Muh Musab M. Ali Albsheer Muzamil Mahdi Abdel Hamid Eun-Taek Han Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan |
topic_facet |
Malaria Plasmodium vivax Sudan Haplotype Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Vivax malaria is a leading public health concern worldwide. Due to the high prevalence of Duffy-negative blood group population, Plasmodium vivax in Africa historically is less attributable and remains a neglected disease. The interaction between Duffy binding protein and its cognate receptor, Duffy antigen receptor for chemokine plays a key role in the invasion of red blood cells and serves as a novel vaccine candidate against P. vivax. However, the polymorphic nature of P. vivax Duffy binding protein (DBP), particularly N-terminal cysteine-rich region (PvDBPII), represents a major obstacle for the successful design of a DBP-based vaccine to enable global protection. In this study, the level of pvdbpII sequence variations, Duffy blood group genotypes, number of haplotypes circulating, and the natural selection at pvdbpII in Sudan isolates were analysed and the implication in terms of DBP-based vaccine design was discussed. Methods Forty-two P. vivax-infected blood samples were collected from patients from different areas of Sudan during 2014–2016. For Duffy blood group genotyping, the fragment that indicates GATA-1 transcription factor binding site of the FY gene (− 33T > C) was amplified by PCR and sequenced by direct sequencing. The region II flanking pvdbpII was PCR amplified and sequenced by direct sequencing. The genetic diversity and natural selection of pvdbpII were done using DnaSP ver 5.0 and MEGA ver 5.0 programs. Based on predominant, non-synonymous, single nucleotide polymorphisms (SNPs), prevalence of Sudanese haplotypes was assessed in global isolates. Results Twenty SNPs (14 non-synonymous and 6 synonymous) were identified in pvdbpII among the 42 Sudan P. vivax isolates. Sequence analysis revealed that 11 different PvDBP haplotypes exist in Sudan P. vivax isolates and the region has evolved under positive selection. Among the identified PvDBP haplotypes five PvDBP haplotypes were shared among Duffy-negative as well as Duffy-positive individuals. The high selective pressure ... |
format |
Article in Journal/Newspaper |
author |
Mohammad Rafiul Hoque Mohammed Mohieldien Abbas Elfaki Md Atique Ahmed Seong-Kyun Lee Fauzi Muh Musab M. Ali Albsheer Muzamil Mahdi Abdel Hamid Eun-Taek Han |
author_facet |
Mohammad Rafiul Hoque Mohammed Mohieldien Abbas Elfaki Md Atique Ahmed Seong-Kyun Lee Fauzi Muh Musab M. Ali Albsheer Muzamil Mahdi Abdel Hamid Eun-Taek Han |
author_sort |
Mohammad Rafiul Hoque |
title |
Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan |
title_short |
Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan |
title_full |
Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan |
title_fullStr |
Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan |
title_full_unstemmed |
Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan |
title_sort |
diversity pattern of duffy binding protein sequence among duffy-negatives and duffy-positives in sudan |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2425-z https://doaj.org/article/735ee02cbb824e769f10d65f2c0b770a |
long_lat |
ENVELOPE(-19.702,-19.702,63.540,63.540) |
geographic |
Arctic Gata |
geographic_facet |
Arctic Gata |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2425-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2425-z 1475-2875 https://doaj.org/article/735ee02cbb824e769f10d65f2c0b770a |
op_doi |
https://doi.org/10.1186/s12936-018-2425-z |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766347521625423872 |