Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4.
Trichinellosis is a serious food-borne parasitic zoonosis worldwide. In the effort to develop vaccine against Trichinella infection, we have identified Trichinella spiralis Heat shock protein 70 (Ts-Hsp70) elicits partial protective immunity against T. spiralis infection via activating dendritic cel...
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ftdoajarticles:oai:doaj.org/article:735c2797ccfa4e11bc8b49c77bc57f92 2023-05-15T15:11:07+02:00 Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. Rui Zhang Qing Sun Yi Chen Ximeng Sun Yuan Gu Zhang Zhao Yuli Cheng Limei Zhao Jingjing Huang Bin Zhan Xinping Zhu 2018-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006502 https://doaj.org/article/735c2797ccfa4e11bc8b49c77bc57f92 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5979045?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006502 https://doaj.org/article/735c2797ccfa4e11bc8b49c77bc57f92 PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006502 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006502 2022-12-31T12:31:34Z Trichinellosis is a serious food-borne parasitic zoonosis worldwide. In the effort to develop vaccine against Trichinella infection, we have identified Trichinella spiralis Heat shock protein 70 (Ts-Hsp70) elicits partial protective immunity against T. spiralis infection via activating dendritic cells (DCs) in our previous study. This study aims to investigate whether DCs were activated by Ts-Hsp70 through TLR2 and/or TLR4 pathways.After blocking with anti-TLR2 and TLR4 antibodies, the binding of Ts-Hsp70 to DCs was significantly reduced. The reduced binding effects were also found in TLR2 and TLR4 knockout (TLR2-/- and TLR4-/-) DCs. The expression of TLR2 and TLR4 on DCs was upregulated after treatment with Ts-Hsp70 in vitro. These results suggest that Ts-Hsp70 is able to directly bind to TLR2 and TLR4 on the surface of mouse bone morrow-derived DCs. In addition, the expression of the co-stimulatory molecules (CD80, CD83) on Ts-Hsp70-induced DCs was reduced in TLR2-/- and TLR4-/- mice. More evidence showed that Ts-Hsp70 reduced its activation on TLR2/4 knockout DCs to subsequently activate the naïve T-cells. Furthermore, Ts-Hsp70 elicited protective immunity against T. spiralis infection was reduced in TLR2-/- and TLR4-/- mice correlating with the reduced humoral and cellular immune responses.This study demonstrates that Ts-Hsp70 activates DCs through TLR2 and TLR4, and TLR2 and TLR4 play important roles in Ts-Hsp70-induced DCs activation and immune responses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Morrow ENVELOPE(-81.566,-81.566,50.550,50.550) PLOS Neglected Tropical Diseases 12 5 e0006502 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rui Zhang Qing Sun Yi Chen Ximeng Sun Yuan Gu Zhang Zhao Yuli Cheng Limei Zhao Jingjing Huang Bin Zhan Xinping Zhu Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trichinellosis is a serious food-borne parasitic zoonosis worldwide. In the effort to develop vaccine against Trichinella infection, we have identified Trichinella spiralis Heat shock protein 70 (Ts-Hsp70) elicits partial protective immunity against T. spiralis infection via activating dendritic cells (DCs) in our previous study. This study aims to investigate whether DCs were activated by Ts-Hsp70 through TLR2 and/or TLR4 pathways.After blocking with anti-TLR2 and TLR4 antibodies, the binding of Ts-Hsp70 to DCs was significantly reduced. The reduced binding effects were also found in TLR2 and TLR4 knockout (TLR2-/- and TLR4-/-) DCs. The expression of TLR2 and TLR4 on DCs was upregulated after treatment with Ts-Hsp70 in vitro. These results suggest that Ts-Hsp70 is able to directly bind to TLR2 and TLR4 on the surface of mouse bone morrow-derived DCs. In addition, the expression of the co-stimulatory molecules (CD80, CD83) on Ts-Hsp70-induced DCs was reduced in TLR2-/- and TLR4-/- mice. More evidence showed that Ts-Hsp70 reduced its activation on TLR2/4 knockout DCs to subsequently activate the naïve T-cells. Furthermore, Ts-Hsp70 elicited protective immunity against T. spiralis infection was reduced in TLR2-/- and TLR4-/- mice correlating with the reduced humoral and cellular immune responses.This study demonstrates that Ts-Hsp70 activates DCs through TLR2 and TLR4, and TLR2 and TLR4 play important roles in Ts-Hsp70-induced DCs activation and immune responses. |
format |
Article in Journal/Newspaper |
author |
Rui Zhang Qing Sun Yi Chen Ximeng Sun Yuan Gu Zhang Zhao Yuli Cheng Limei Zhao Jingjing Huang Bin Zhan Xinping Zhu |
author_facet |
Rui Zhang Qing Sun Yi Chen Ximeng Sun Yuan Gu Zhang Zhao Yuli Cheng Limei Zhao Jingjing Huang Bin Zhan Xinping Zhu |
author_sort |
Rui Zhang |
title |
Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. |
title_short |
Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. |
title_full |
Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. |
title_fullStr |
Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. |
title_full_unstemmed |
Ts-Hsp70 induces protective immunity against Trichinella spiralis infection in mouse by activating dendritic cells through TLR2 and TLR4. |
title_sort |
ts-hsp70 induces protective immunity against trichinella spiralis infection in mouse by activating dendritic cells through tlr2 and tlr4. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006502 https://doaj.org/article/735c2797ccfa4e11bc8b49c77bc57f92 |
long_lat |
ENVELOPE(-81.566,-81.566,50.550,50.550) |
geographic |
Arctic Morrow |
geographic_facet |
Arctic Morrow |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006502 (2018) |
op_relation |
http://europepmc.org/articles/PMC5979045?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006502 https://doaj.org/article/735c2797ccfa4e11bc8b49c77bc57f92 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006502 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
5 |
container_start_page |
e0006502 |
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