IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration
One impediment to the successful oral vaccination in fish is the hostile stomach environment that antigens must cross. Furthermore, uptake of antigens from the gut to systemic distribution is required for induction of systemic immunity, the dynamics of which are poorly understood. In the present stu...
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ftdoajarticles:oai:doaj.org/article:7320f4a7d8a641589973e9e7d0b8d379 2023-05-15T15:29:58+02:00 IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration Lihan Chen Øystein Evensen Stephen Mutoloki 2015-05-01T00:00:00Z https://doi.org/10.3390/v7052507 https://doaj.org/article/7320f4a7d8a641589973e9e7d0b8d379 EN eng MDPI AG http://www.mdpi.com/1999-4915/7/5/2507 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v7052507 https://doaj.org/article/7320f4a7d8a641589973e9e7d0b8d379 Viruses, Vol 7, Iss 5, Pp 2507-2517 (2015) infectious pancreatic necrosis virus (IPNV) Atlantic salmon oral anal uptake Microbiology QR1-502 article 2015 ftdoajarticles https://doi.org/10.3390/v7052507 2022-12-31T04:44:11Z One impediment to the successful oral vaccination in fish is the hostile stomach environment that antigens must cross. Furthermore, uptake of antigens from the gut to systemic distribution is required for induction of systemic immunity, the dynamics of which are poorly understood. In the present study, groups of Atlantic salmon parr were intubated with live or inactivated infectious pancreatic necrosis virus (IPNV), either orally or anally. At 1, 24 and 72 h post infection (p.i.), the fish were sacrificed. Serum was used for assessing IPNV by ELISA, while formalin-fixed head-kidney, spleen, liver and intestine tissues were used for the demonstration of antigens by immunohistochemistry. Both live and inactivated IPNV antigens were observed in enterocytes of the intestines and in immune cells of the head-kidneys and spleens of all groups. In the liver, no antigens were observed in any of the groups. Significantly higher serum antigen OD values (p < 0.04) were observed in orally- compared to anally-intubated fish. By contrast, no difference (p = 0.05) was observed in tissue antigens between these groups by immunohistochemistry. No significant difference (p = 0.05) in serum antigens was observed between groups intubated with live and inactivated IPNV, while in tissues, significantly more antigens (p < 0.03) were observe in the latter compared to the former. These findings demonstrate that both live and inactivated IPNV are taken up by enterocytes in the intestines of Atlantic salmon, likely by receptor-mediated mechanisms. Higher IPNV uptake by the oral compared to anal route suggests that both the anterior and posterior intestines are important for the uptake of the virus and that IPNV is resistant to gastric degradation of the Atlantic salmon stomach. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Viruses 7 5 2507 2517 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
infectious pancreatic necrosis virus (IPNV) Atlantic salmon oral anal uptake Microbiology QR1-502 |
spellingShingle |
infectious pancreatic necrosis virus (IPNV) Atlantic salmon oral anal uptake Microbiology QR1-502 Lihan Chen Øystein Evensen Stephen Mutoloki IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration |
topic_facet |
infectious pancreatic necrosis virus (IPNV) Atlantic salmon oral anal uptake Microbiology QR1-502 |
description |
One impediment to the successful oral vaccination in fish is the hostile stomach environment that antigens must cross. Furthermore, uptake of antigens from the gut to systemic distribution is required for induction of systemic immunity, the dynamics of which are poorly understood. In the present study, groups of Atlantic salmon parr were intubated with live or inactivated infectious pancreatic necrosis virus (IPNV), either orally or anally. At 1, 24 and 72 h post infection (p.i.), the fish were sacrificed. Serum was used for assessing IPNV by ELISA, while formalin-fixed head-kidney, spleen, liver and intestine tissues were used for the demonstration of antigens by immunohistochemistry. Both live and inactivated IPNV antigens were observed in enterocytes of the intestines and in immune cells of the head-kidneys and spleens of all groups. In the liver, no antigens were observed in any of the groups. Significantly higher serum antigen OD values (p < 0.04) were observed in orally- compared to anally-intubated fish. By contrast, no difference (p = 0.05) was observed in tissue antigens between these groups by immunohistochemistry. No significant difference (p = 0.05) in serum antigens was observed between groups intubated with live and inactivated IPNV, while in tissues, significantly more antigens (p < 0.03) were observe in the latter compared to the former. These findings demonstrate that both live and inactivated IPNV are taken up by enterocytes in the intestines of Atlantic salmon, likely by receptor-mediated mechanisms. Higher IPNV uptake by the oral compared to anal route suggests that both the anterior and posterior intestines are important for the uptake of the virus and that IPNV is resistant to gastric degradation of the Atlantic salmon stomach. |
format |
Article in Journal/Newspaper |
author |
Lihan Chen Øystein Evensen Stephen Mutoloki |
author_facet |
Lihan Chen Øystein Evensen Stephen Mutoloki |
author_sort |
Lihan Chen |
title |
IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration |
title_short |
IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration |
title_full |
IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration |
title_fullStr |
IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration |
title_full_unstemmed |
IPNV Antigen Uptake and Distribution in Atlantic Salmon Following Oral Administration |
title_sort |
ipnv antigen uptake and distribution in atlantic salmon following oral administration |
publisher |
MDPI AG |
publishDate |
2015 |
url |
https://doi.org/10.3390/v7052507 https://doaj.org/article/7320f4a7d8a641589973e9e7d0b8d379 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Viruses, Vol 7, Iss 5, Pp 2507-2517 (2015) |
op_relation |
http://www.mdpi.com/1999-4915/7/5/2507 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v7052507 https://doaj.org/article/7320f4a7d8a641589973e9e7d0b8d379 |
op_doi |
https://doi.org/10.3390/v7052507 |
container_title |
Viruses |
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7 |
container_issue |
5 |
container_start_page |
2507 |
op_container_end_page |
2517 |
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1766360419935453184 |