Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria
Objective: To investigate the antioxidant, antibacterial and phytochemical properties of ethanol extracts of Brachylaena elliptica and Brachylaena ilicifolia against wound infecting bacteria normally found in diabetic patients. Methods: The in vitro antioxidant activity of the two plants extracts we...
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ftdoajarticles:oai:doaj.org/article:72dbdb0b54724fabaff942f94169cd80 2023-05-15T15:11:05+02:00 Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria Idowu Jonas Sagbo Anthony Jide Afolayan Graeme Bradley 2017-09-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.08.009 https://doaj.org/article/72dbdb0b54724fabaff942f94169cd80 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117308535 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.08.009 https://doaj.org/article/72dbdb0b54724fabaff942f94169cd80 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 9, Pp 817-825 (2017) Diabetes mellitus Antioxidant Phytochemical Brachylaena elliptica Brachylaena ilicifolia Wound Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.08.009 2022-12-31T08:15:18Z Objective: To investigate the antioxidant, antibacterial and phytochemical properties of ethanol extracts of Brachylaena elliptica and Brachylaena ilicifolia against wound infecting bacteria normally found in diabetic patients. Methods: The in vitro antioxidant activity of the two plants extracts were investigated spectrophotometrically using 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt, hydrogen peroxide (H2O2) and ferric reducing power. The antibacterial assay and minimum inhibitory concentration (MIC) was determined using the agar dilution method against five bacteria strains using amoxycillin and ciprofloxacin as positive control. The phytochemical analyses (tannins, total phenol, flavonoids, flavonols, proanthocyanidin, alkaloids and saponins) were assessed using standard methods. Results: The ethanol extract of both plants exhibited strong antioxidant activities in some cases when compared to the standards (vitamin C and BHT). The antibacterial activity of both plants showed an appreciable broad spectrum activity against these wound pathogens with MIC value ranges between 0.3 mg/mL and 5 mg/mL. Tannins, phenols, flavanols, proanthocyanidins and alkaloids content of B. ilicifolia were significantly higher than those in B. elliptica. However, there were no significant differences in the flavanoid content of both plants extracts. Conclusions: These results indicated that the ethanol leaf extracts of these plants have antioxidant and antibacterial activity against the tested bacteria possibly due to the presence of bioactive compounds and therefore could be used as alternative therapy against wound infection caused by these bacteria in diabetic patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 9 817 825 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Diabetes mellitus Antioxidant Phytochemical Brachylaena elliptica Brachylaena ilicifolia Wound Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Diabetes mellitus Antioxidant Phytochemical Brachylaena elliptica Brachylaena ilicifolia Wound Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Idowu Jonas Sagbo Anthony Jide Afolayan Graeme Bradley Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
topic_facet |
Diabetes mellitus Antioxidant Phytochemical Brachylaena elliptica Brachylaena ilicifolia Wound Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antioxidant, antibacterial and phytochemical properties of ethanol extracts of Brachylaena elliptica and Brachylaena ilicifolia against wound infecting bacteria normally found in diabetic patients. Methods: The in vitro antioxidant activity of the two plants extracts were investigated spectrophotometrically using 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt, hydrogen peroxide (H2O2) and ferric reducing power. The antibacterial assay and minimum inhibitory concentration (MIC) was determined using the agar dilution method against five bacteria strains using amoxycillin and ciprofloxacin as positive control. The phytochemical analyses (tannins, total phenol, flavonoids, flavonols, proanthocyanidin, alkaloids and saponins) were assessed using standard methods. Results: The ethanol extract of both plants exhibited strong antioxidant activities in some cases when compared to the standards (vitamin C and BHT). The antibacterial activity of both plants showed an appreciable broad spectrum activity against these wound pathogens with MIC value ranges between 0.3 mg/mL and 5 mg/mL. Tannins, phenols, flavanols, proanthocyanidins and alkaloids content of B. ilicifolia were significantly higher than those in B. elliptica. However, there were no significant differences in the flavanoid content of both plants extracts. Conclusions: These results indicated that the ethanol leaf extracts of these plants have antioxidant and antibacterial activity against the tested bacteria possibly due to the presence of bioactive compounds and therefore could be used as alternative therapy against wound infection caused by these bacteria in diabetic patients. |
format |
Article in Journal/Newspaper |
author |
Idowu Jonas Sagbo Anthony Jide Afolayan Graeme Bradley |
author_facet |
Idowu Jonas Sagbo Anthony Jide Afolayan Graeme Bradley |
author_sort |
Idowu Jonas Sagbo |
title |
Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
title_short |
Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
title_full |
Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
title_fullStr |
Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
title_full_unstemmed |
Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
title_sort |
antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.08.009 https://doaj.org/article/72dbdb0b54724fabaff942f94169cd80 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 9, Pp 817-825 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117308535 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.08.009 https://doaj.org/article/72dbdb0b54724fabaff942f94169cd80 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.08.009 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
9 |
container_start_page |
817 |
op_container_end_page |
825 |
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1766341992029093888 |