Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach
A highly effective method was developed to functionalize ceramic supports (Al 2 O 3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evalu...
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ftdoajarticles:oai:doaj.org/article:72be9dfd7e3a445984ba4552c848cc1c 2023-05-15T13:59:54+02:00 Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach Joanna Kujawa Marta Głodek Izabela Koter Borys Ośmiałowski Katarzyna Knozowska Samer Al-Gharabli Ludovic F. Dumée Wojciech Kujawski 2021-01-01T00:00:00Z https://doi.org/10.3390/ma14010201 https://doaj.org/article/72be9dfd7e3a445984ba4552c848cc1c EN eng MDPI AG https://www.mdpi.com/1996-1944/14/1/201 https://doaj.org/toc/1996-1944 doi:10.3390/ma14010201 1996-1944 https://doaj.org/article/72be9dfd7e3a445984ba4552c848cc1c Materials, Vol 14, Iss 201, p 201 (2021) surface modification organic spacer enzyme immobilization Candida antarctica lipase B ceramic membranes alumina oxide Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 article 2021 ftdoajarticles https://doi.org/10.3390/ma14010201 2022-12-31T05:23:07Z A highly effective method was developed to functionalize ceramic supports (Al 2 O 3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Materials 14 1 201 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
surface modification organic spacer enzyme immobilization Candida antarctica lipase B ceramic membranes alumina oxide Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 |
spellingShingle |
surface modification organic spacer enzyme immobilization Candida antarctica lipase B ceramic membranes alumina oxide Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Joanna Kujawa Marta Głodek Izabela Koter Borys Ośmiałowski Katarzyna Knozowska Samer Al-Gharabli Ludovic F. Dumée Wojciech Kujawski Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach |
topic_facet |
surface modification organic spacer enzyme immobilization Candida antarctica lipase B ceramic membranes alumina oxide Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 |
description |
A highly effective method was developed to functionalize ceramic supports (Al 2 O 3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules. |
format |
Article in Journal/Newspaper |
author |
Joanna Kujawa Marta Głodek Izabela Koter Borys Ośmiałowski Katarzyna Knozowska Samer Al-Gharabli Ludovic F. Dumée Wojciech Kujawski |
author_facet |
Joanna Kujawa Marta Głodek Izabela Koter Borys Ośmiałowski Katarzyna Knozowska Samer Al-Gharabli Ludovic F. Dumée Wojciech Kujawski |
author_sort |
Joanna Kujawa |
title |
Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach |
title_short |
Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach |
title_full |
Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach |
title_fullStr |
Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach |
title_full_unstemmed |
Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach |
title_sort |
molecular decoration of ceramic supports for highly effective enzyme immobilization—material approach |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ma14010201 https://doaj.org/article/72be9dfd7e3a445984ba4552c848cc1c |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Materials, Vol 14, Iss 201, p 201 (2021) |
op_relation |
https://www.mdpi.com/1996-1944/14/1/201 https://doaj.org/toc/1996-1944 doi:10.3390/ma14010201 1996-1944 https://doaj.org/article/72be9dfd7e3a445984ba4552c848cc1c |
op_doi |
https://doi.org/10.3390/ma14010201 |
container_title |
Materials |
container_volume |
14 |
container_issue |
1 |
container_start_page |
201 |
_version_ |
1766268829426515968 |