Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development
Abstract Background The complex life cycle of malaria parasites requires well-orchestrated stage specific gene expression. In the vertebrate host the parasites grow and multiply by schizogony in two different environments: within erythrocytes and within hepatocytes. Whereas erythrocytic parasites ar...
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ftdoajarticles:oai:doaj.org/article:728e1abb33eb456fb3fcb7e9917f8a35 2023-05-15T15:15:42+02:00 Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development Reto Caldelari Sunil Dogga Marc W. Schmid Blandine Franke-Fayard Chris J. Janse Dominique Soldati-Favre Volker Heussler 2019-09-01T00:00:00Z https://doi.org/10.1186/s12936-019-2968-7 https://doaj.org/article/728e1abb33eb456fb3fcb7e9917f8a35 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2968-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2968-7 1475-2875 https://doaj.org/article/728e1abb33eb456fb3fcb7e9917f8a35 Malaria Journal, Vol 18, Iss 1, Pp 1-20 (2019) Malaria Life cycle Plasmodium liver stage Transcriptome RNA-seq Merosome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2968-7 2022-12-31T07:08:20Z Abstract Background The complex life cycle of malaria parasites requires well-orchestrated stage specific gene expression. In the vertebrate host the parasites grow and multiply by schizogony in two different environments: within erythrocytes and within hepatocytes. Whereas erythrocytic parasites are well-studied in this respect, relatively little is known about the exo-erythrocytic stages. Methods In an attempt to fill this gap, genome wide RNA-seq analyses of various exo-erythrocytic stages of Plasmodium berghei including sporozoites, samples from a time-course of liver stage development and detached cells were performed. These latter contain infectious merozoites and represent the final step in exo-erythrocytic development. Results The analysis represents the complete transcriptome of the entire life cycle of P. berghei parasites with temporal detailed analysis of the liver stage allowing comparison of gene expression across the progression of the life cycle. These RNA-seq data from different developmental stages were used to cluster genes with similar expression profiles, in order to infer their functions. A comparison with published data from other parasite stages confirmed stage-specific gene expression and revealed numerous genes that are expressed differentially in blood and exo-erythrocytic stages. One of the most exo-erythrocytic stage-specific genes was PBANKA_1003900, which has previously been annotated as a “gametocyte specific protein”. The promoter of this gene drove high GFP expression in exo-erythrocytic stages, confirming its expression profile seen by RNA-seq. Conclusions The comparative analysis of the genome wide mRNA expression profiles of erythrocytic and different exo-erythrocytic stages could be used to improve the understanding of gene regulation in Plasmodium parasites and can be used to model exo-erythrocytic stage metabolic networks toward the identification of differences in metabolic processes during schizogony in erythrocytes and hepatocytes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Malaria Life cycle Plasmodium liver stage Transcriptome RNA-seq Merosome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Life cycle Plasmodium liver stage Transcriptome RNA-seq Merosome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Reto Caldelari Sunil Dogga Marc W. Schmid Blandine Franke-Fayard Chris J. Janse Dominique Soldati-Favre Volker Heussler Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development |
topic_facet |
Malaria Life cycle Plasmodium liver stage Transcriptome RNA-seq Merosome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The complex life cycle of malaria parasites requires well-orchestrated stage specific gene expression. In the vertebrate host the parasites grow and multiply by schizogony in two different environments: within erythrocytes and within hepatocytes. Whereas erythrocytic parasites are well-studied in this respect, relatively little is known about the exo-erythrocytic stages. Methods In an attempt to fill this gap, genome wide RNA-seq analyses of various exo-erythrocytic stages of Plasmodium berghei including sporozoites, samples from a time-course of liver stage development and detached cells were performed. These latter contain infectious merozoites and represent the final step in exo-erythrocytic development. Results The analysis represents the complete transcriptome of the entire life cycle of P. berghei parasites with temporal detailed analysis of the liver stage allowing comparison of gene expression across the progression of the life cycle. These RNA-seq data from different developmental stages were used to cluster genes with similar expression profiles, in order to infer their functions. A comparison with published data from other parasite stages confirmed stage-specific gene expression and revealed numerous genes that are expressed differentially in blood and exo-erythrocytic stages. One of the most exo-erythrocytic stage-specific genes was PBANKA_1003900, which has previously been annotated as a “gametocyte specific protein”. The promoter of this gene drove high GFP expression in exo-erythrocytic stages, confirming its expression profile seen by RNA-seq. Conclusions The comparative analysis of the genome wide mRNA expression profiles of erythrocytic and different exo-erythrocytic stages could be used to improve the understanding of gene regulation in Plasmodium parasites and can be used to model exo-erythrocytic stage metabolic networks toward the identification of differences in metabolic processes during schizogony in erythrocytes and hepatocytes. |
format |
Article in Journal/Newspaper |
author |
Reto Caldelari Sunil Dogga Marc W. Schmid Blandine Franke-Fayard Chris J. Janse Dominique Soldati-Favre Volker Heussler |
author_facet |
Reto Caldelari Sunil Dogga Marc W. Schmid Blandine Franke-Fayard Chris J. Janse Dominique Soldati-Favre Volker Heussler |
author_sort |
Reto Caldelari |
title |
Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development |
title_short |
Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development |
title_full |
Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development |
title_fullStr |
Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development |
title_full_unstemmed |
Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development |
title_sort |
transcriptome analysis of plasmodium berghei during exo-erythrocytic development |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2968-7 https://doaj.org/article/728e1abb33eb456fb3fcb7e9917f8a35 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-20 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2968-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2968-7 1475-2875 https://doaj.org/article/728e1abb33eb456fb3fcb7e9917f8a35 |
op_doi |
https://doi.org/10.1186/s12936-019-2968-7 |
container_title |
Malaria Journal |
container_volume |
18 |
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1 |
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1766346039641505792 |