An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses
Abstract Influenza viruses are an important cause of disease in both humans and animals, and their detection and characterization can take weeks. In this study, we sought to compare classical virology techniques with a new rapid microarray method for the detection and characterization of a very dive...
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ftdoajarticles:oai:doaj.org/article:72502165463b425ca9d2a90a460bab33 2023-05-15T15:15:23+02:00 An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses Emily S. Bailey Xinye Wang Mai-juan Ma Guo-lin Wang Gregory C. Gray 2021-03-01T00:00:00Z https://doi.org/10.1186/s40794-021-00133-7 https://doaj.org/article/72502165463b425ca9d2a90a460bab33 EN eng BMC https://doi.org/10.1186/s40794-021-00133-7 https://doaj.org/toc/2055-0936 doi:10.1186/s40794-021-00133-7 2055-0936 https://doaj.org/article/72502165463b425ca9d2a90a460bab33 Tropical Diseases, Travel Medicine and Vaccines, Vol 7, Iss 1, Pp 1-5 (2021) Clinical performance Diagnostic validation FluChip-8G insight assay Influenza subtyping Microarray Multiplex RT-PCR Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.1186/s40794-021-00133-7 2022-12-31T06:14:05Z Abstract Influenza viruses are an important cause of disease in both humans and animals, and their detection and characterization can take weeks. In this study, we sought to compare classical virology techniques with a new rapid microarray method for the detection and characterization of a very diverse, panel of animal, environmental, and human clinical or field specimens that were molecularly positive for influenza A alone (n = 111), influenza B alone (n = 3), both viruses (n = 13), or influenza negative (n = 2) viruses. All influenza virus positive samples in this study were first subtyped by traditional laboratory methods, and later evaluated using the FluChip-8G Insight Assay (InDevR Inc. Boulder, CO) in laboratories at Duke University (USA) or at Duke Kunshan University (China). The FluChip-8G Insight multiplexed assay agreed with classical virologic techniques 59 (54.1%) of 109 influenza A-positive, 3 (100%) of the 3 influenza B-positive, 0 (0%) of 10 both influenza A- and B-positive samples, 75% of 24 environmental samples including those positive for H1, H3, H7, H9, N1, and N9 strains, and 80% of 22 avian influenza samples. It had difficulty with avian N6 types and swine H3 and N2 influenza specimens. The FluChip-8G Insight assay performed well with most human, environmental, and animal samples, but had some difficulty with samples containing multiple viral strains and with specific animal influenza strains. As classical virology methods are often iterative and can take weeks, the FluChip-8G Insight Assay rapid results (time range 8 to 12 h) offers considerable time savings. As the FluChip-8G analysis algorithm is expected to improve over time with addition of new subtypes and sample matrices, the FluChip-8G Insight Assay has considerable promise for rapid characterization of novel influenza viruses affecting humans or animals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Diseases, Travel Medicine and Vaccines 7 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Clinical performance Diagnostic validation FluChip-8G insight assay Influenza subtyping Microarray Multiplex RT-PCR Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Clinical performance Diagnostic validation FluChip-8G insight assay Influenza subtyping Microarray Multiplex RT-PCR Arctic medicine. Tropical medicine RC955-962 Emily S. Bailey Xinye Wang Mai-juan Ma Guo-lin Wang Gregory C. Gray An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses |
topic_facet |
Clinical performance Diagnostic validation FluChip-8G insight assay Influenza subtyping Microarray Multiplex RT-PCR Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Influenza viruses are an important cause of disease in both humans and animals, and their detection and characterization can take weeks. In this study, we sought to compare classical virology techniques with a new rapid microarray method for the detection and characterization of a very diverse, panel of animal, environmental, and human clinical or field specimens that were molecularly positive for influenza A alone (n = 111), influenza B alone (n = 3), both viruses (n = 13), or influenza negative (n = 2) viruses. All influenza virus positive samples in this study were first subtyped by traditional laboratory methods, and later evaluated using the FluChip-8G Insight Assay (InDevR Inc. Boulder, CO) in laboratories at Duke University (USA) or at Duke Kunshan University (China). The FluChip-8G Insight multiplexed assay agreed with classical virologic techniques 59 (54.1%) of 109 influenza A-positive, 3 (100%) of the 3 influenza B-positive, 0 (0%) of 10 both influenza A- and B-positive samples, 75% of 24 environmental samples including those positive for H1, H3, H7, H9, N1, and N9 strains, and 80% of 22 avian influenza samples. It had difficulty with avian N6 types and swine H3 and N2 influenza specimens. The FluChip-8G Insight assay performed well with most human, environmental, and animal samples, but had some difficulty with samples containing multiple viral strains and with specific animal influenza strains. As classical virology methods are often iterative and can take weeks, the FluChip-8G Insight Assay rapid results (time range 8 to 12 h) offers considerable time savings. As the FluChip-8G analysis algorithm is expected to improve over time with addition of new subtypes and sample matrices, the FluChip-8G Insight Assay has considerable promise for rapid characterization of novel influenza viruses affecting humans or animals. |
format |
Article in Journal/Newspaper |
author |
Emily S. Bailey Xinye Wang Mai-juan Ma Guo-lin Wang Gregory C. Gray |
author_facet |
Emily S. Bailey Xinye Wang Mai-juan Ma Guo-lin Wang Gregory C. Gray |
author_sort |
Emily S. Bailey |
title |
An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses |
title_short |
An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses |
title_full |
An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses |
title_fullStr |
An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses |
title_full_unstemmed |
An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses |
title_sort |
evaluation of the indevr fluchip-8g insight microarray assay in characterizing influenza a viruses |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s40794-021-00133-7 https://doaj.org/article/72502165463b425ca9d2a90a460bab33 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Diseases, Travel Medicine and Vaccines, Vol 7, Iss 1, Pp 1-5 (2021) |
op_relation |
https://doi.org/10.1186/s40794-021-00133-7 https://doaj.org/toc/2055-0936 doi:10.1186/s40794-021-00133-7 2055-0936 https://doaj.org/article/72502165463b425ca9d2a90a460bab33 |
op_doi |
https://doi.org/10.1186/s40794-021-00133-7 |
container_title |
Tropical Diseases, Travel Medicine and Vaccines |
container_volume |
7 |
container_issue |
1 |
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1766345744921395200 |