SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas
The self-renewal and differentiation of germ cells are essential for gametogenesis and reproduction. In mammals, the transcription factors SOHLH1 and SOHLH2, two members of the bHLH family, are specifically expressed in the gonads, and play an important role in spermatocyte and oocyte differentiatio...
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ftdoajarticles:oai:doaj.org/article:722fcf302b3b44eeb20eb48220265c73 2023-05-15T15:57:54+02:00 SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas Guang Qian Yongbo Bao Danli Song Na Chen Zhihua Lin 2019-05-01T00:00:00Z https://doi.org/10.3389/fphys.2019.00594 https://doaj.org/article/722fcf302b3b44eeb20eb48220265c73 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fphys.2019.00594/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2019.00594 https://doaj.org/article/722fcf302b3b44eeb20eb48220265c73 Frontiers in Physiology, Vol 10 (2019) Crassostrea gigas SOHLH phylogenetics gametogenesis expression Physiology QP1-981 article 2019 ftdoajarticles https://doi.org/10.3389/fphys.2019.00594 2022-12-31T09:41:12Z The self-renewal and differentiation of germ cells are essential for gametogenesis and reproduction. In mammals, the transcription factors SOHLH1 and SOHLH2, two members of the bHLH family, are specifically expressed in the gonads, and play an important role in spermatocyte and oocyte differentiation. In our previous study, we performed a phylogenetic analysis of the Lophotrochozoa bHLH genes, and two Sohlh were identified in the Pacific oyster Crassostrea gigas. Based on the genomes of other species that have complete genomic information, we further analyzed the phylogenetics of the Sohlh in this study. The results indicate that the Sohlh are ancient genes that were lost in many species during evolution, including in some invertebrates, and lower vertebrates. The phylogenetic tree shows that Sohlh1 and Sohlh2 are located in different scaffolds and that they have low similarity, suggesting early separation in invertebrates. We used RNA-seq and RT-PCR to examine the mRNA expression of the Sohlh in C. gigas (termed Cg-Sohlh), we found that Cg-Sohlh1, and Cg-Sohlh2 are specifically expressed in the gonads. During gonadal development, the mRNA expression levels of both genes increased from the proliferative stage and reached the highest level at the growth stage (P < 0.05). Then, the expression level decreased until the resting stage. In addition, immunohistochemistry was used to determine that the Cg-SOHLH1 protein was specifically expressed in the spermatogonia and spermatocytes. Cg-Sohlh2 mRNA was expressed in both the male and female gonads, while Cg-Sohlh1 mRNA was highly expressed in the female gonads at all developmental stages except for the resting stage. These data indicate that Cg-SOHLH might be gonad-specific regulatory factors, similar to mammalian SOHLH, and that Cg-SOHLH1 might be involved in spermatogonial differentiation. This study lays the foundation to further determine the functional role of SOHLH in mollusk gametogenesis and provides a foundation to better understand the regulatory mechanism ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Physiology 10 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Crassostrea gigas SOHLH phylogenetics gametogenesis expression Physiology QP1-981 |
spellingShingle |
Crassostrea gigas SOHLH phylogenetics gametogenesis expression Physiology QP1-981 Guang Qian Yongbo Bao Danli Song Na Chen Zhihua Lin SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas |
topic_facet |
Crassostrea gigas SOHLH phylogenetics gametogenesis expression Physiology QP1-981 |
description |
The self-renewal and differentiation of germ cells are essential for gametogenesis and reproduction. In mammals, the transcription factors SOHLH1 and SOHLH2, two members of the bHLH family, are specifically expressed in the gonads, and play an important role in spermatocyte and oocyte differentiation. In our previous study, we performed a phylogenetic analysis of the Lophotrochozoa bHLH genes, and two Sohlh were identified in the Pacific oyster Crassostrea gigas. Based on the genomes of other species that have complete genomic information, we further analyzed the phylogenetics of the Sohlh in this study. The results indicate that the Sohlh are ancient genes that were lost in many species during evolution, including in some invertebrates, and lower vertebrates. The phylogenetic tree shows that Sohlh1 and Sohlh2 are located in different scaffolds and that they have low similarity, suggesting early separation in invertebrates. We used RNA-seq and RT-PCR to examine the mRNA expression of the Sohlh in C. gigas (termed Cg-Sohlh), we found that Cg-Sohlh1, and Cg-Sohlh2 are specifically expressed in the gonads. During gonadal development, the mRNA expression levels of both genes increased from the proliferative stage and reached the highest level at the growth stage (P < 0.05). Then, the expression level decreased until the resting stage. In addition, immunohistochemistry was used to determine that the Cg-SOHLH1 protein was specifically expressed in the spermatogonia and spermatocytes. Cg-Sohlh2 mRNA was expressed in both the male and female gonads, while Cg-Sohlh1 mRNA was highly expressed in the female gonads at all developmental stages except for the resting stage. These data indicate that Cg-SOHLH might be gonad-specific regulatory factors, similar to mammalian SOHLH, and that Cg-SOHLH1 might be involved in spermatogonial differentiation. This study lays the foundation to further determine the functional role of SOHLH in mollusk gametogenesis and provides a foundation to better understand the regulatory mechanism ... |
format |
Article in Journal/Newspaper |
author |
Guang Qian Yongbo Bao Danli Song Na Chen Zhihua Lin |
author_facet |
Guang Qian Yongbo Bao Danli Song Na Chen Zhihua Lin |
author_sort |
Guang Qian |
title |
SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas |
title_short |
SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas |
title_full |
SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas |
title_fullStr |
SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas |
title_full_unstemmed |
SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas |
title_sort |
sohlhs might be gametogenesis-specific bhlh transcriptional regulation factors in crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fphys.2019.00594 https://doaj.org/article/722fcf302b3b44eeb20eb48220265c73 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Physiology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fphys.2019.00594/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2019.00594 https://doaj.org/article/722fcf302b3b44eeb20eb48220265c73 |
op_doi |
https://doi.org/10.3389/fphys.2019.00594 |
container_title |
Frontiers in Physiology |
container_volume |
10 |
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1766393614812839936 |