Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis.
BACKGROUND: Protection and recovery from visceral leishmaniasis (VL) have been associated with cell-mediated immune (CMI) responses, whereas no protective role has been attributed to humoral responses against specific parasitic antigens. In this report, we compared carefully selected groups of indiv...
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ftdoajarticles:oai:doaj.org/article:721b55354aab468a8858c1fc67c197d9 2023-05-15T15:12:13+02:00 Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. Daniel R Abánades Leonardo V Arruda Elaine S Arruda José Roberto A S Pinto Mario S Palma Dorlene Aquino Arlene J Caldas Manuel Soto Aldina Barral Manoel Barral-Netto 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001687 https://doaj.org/article/721b55354aab468a8858c1fc67c197d9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3378602?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001687 https://doaj.org/article/721b55354aab468a8858c1fc67c197d9 PLoS Neglected Tropical Diseases, Vol 6, Iss 6, p e1687 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001687 2022-12-31T13:18:25Z BACKGROUND: Protection and recovery from visceral leishmaniasis (VL) have been associated with cell-mediated immune (CMI) responses, whereas no protective role has been attributed to humoral responses against specific parasitic antigens. In this report, we compared carefully selected groups of individuals with distinct responses to Leishmania chagasi to explore antigen-recognizing IgG present in resistant individuals. METHODOLOGY AND PRINCIPAL FINDINGS: VL patients with negative delayed-type hypersensitivity (DTH) were classified into the susceptible group. Individuals who had recovered from VL and converted to a DTH+ response, as well as asymptomatic infected individuals (DTH+), were categorized into the resistant group. Sera from these groups were used to detect antigens from L. chagasi by conventional and 2D Western blot assays. Despite an overall reduction in the reactivity of several proteins after DTH conversion, a specific group of proteins (approximately 110-130 kDa) consistently reacted with sera from DTH converters. Other antigens that specifically reacted with sera from DTH+ individuals were isolated and tandem mass spectrometry followed by database query with the protein search engine MASCO were used to identify antigens. The serological properties of recombinant version of the selected antigens were tested by ELISA. Sera from asymptomatic infected people (DTH+) reacted more strongly with a mixture of selected recombinant antigens than with total soluble Leishmania antigen (SLA), with less cross-reactivity against Chagas disease patients' sera. SIGNIFICANCE: Our results are the first evidence of leishmania proteins that are specifically recognized by sera from individuals who are putatively resistant to VL. In addition, these data highlight the possibility of using specific proteins in serological tests for the identification of asymptomatic infected individuals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 6 e1687 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniel R Abánades Leonardo V Arruda Elaine S Arruda José Roberto A S Pinto Mario S Palma Dorlene Aquino Arlene J Caldas Manuel Soto Aldina Barral Manoel Barral-Netto Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Protection and recovery from visceral leishmaniasis (VL) have been associated with cell-mediated immune (CMI) responses, whereas no protective role has been attributed to humoral responses against specific parasitic antigens. In this report, we compared carefully selected groups of individuals with distinct responses to Leishmania chagasi to explore antigen-recognizing IgG present in resistant individuals. METHODOLOGY AND PRINCIPAL FINDINGS: VL patients with negative delayed-type hypersensitivity (DTH) were classified into the susceptible group. Individuals who had recovered from VL and converted to a DTH+ response, as well as asymptomatic infected individuals (DTH+), were categorized into the resistant group. Sera from these groups were used to detect antigens from L. chagasi by conventional and 2D Western blot assays. Despite an overall reduction in the reactivity of several proteins after DTH conversion, a specific group of proteins (approximately 110-130 kDa) consistently reacted with sera from DTH converters. Other antigens that specifically reacted with sera from DTH+ individuals were isolated and tandem mass spectrometry followed by database query with the protein search engine MASCO were used to identify antigens. The serological properties of recombinant version of the selected antigens were tested by ELISA. Sera from asymptomatic infected people (DTH+) reacted more strongly with a mixture of selected recombinant antigens than with total soluble Leishmania antigen (SLA), with less cross-reactivity against Chagas disease patients' sera. SIGNIFICANCE: Our results are the first evidence of leishmania proteins that are specifically recognized by sera from individuals who are putatively resistant to VL. In addition, these data highlight the possibility of using specific proteins in serological tests for the identification of asymptomatic infected individuals. |
format |
Article in Journal/Newspaper |
author |
Daniel R Abánades Leonardo V Arruda Elaine S Arruda José Roberto A S Pinto Mario S Palma Dorlene Aquino Arlene J Caldas Manuel Soto Aldina Barral Manoel Barral-Netto |
author_facet |
Daniel R Abánades Leonardo V Arruda Elaine S Arruda José Roberto A S Pinto Mario S Palma Dorlene Aquino Arlene J Caldas Manuel Soto Aldina Barral Manoel Barral-Netto |
author_sort |
Daniel R Abánades |
title |
Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. |
title_short |
Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. |
title_full |
Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. |
title_fullStr |
Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. |
title_full_unstemmed |
Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. |
title_sort |
immunodominant antigens of leishmania chagasi associated with protection against human visceral leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001687 https://doaj.org/article/721b55354aab468a8858c1fc67c197d9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 6, p e1687 (2012) |
op_relation |
http://europepmc.org/articles/PMC3378602?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001687 https://doaj.org/article/721b55354aab468a8858c1fc67c197d9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001687 |
container_title |
PLoS Neglected Tropical Diseases |
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6 |
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6 |
container_start_page |
e1687 |
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1766342938624786432 |