Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs
Lake Fryxell, situated in the McMurdo Dry Valleys of Antarctica, is an intriguing aquatic ecosystem because of its perennial ice cover, highly stratified water column, and extreme physicochemical conditions, which collectively restrict lake biodiversity to solely microbial forms. To expand our curre...
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ftdoajarticles:oai:doaj.org/article:71d11a18b95b484ca5b21806431b9cd9 2023-05-15T13:32:27+02:00 Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs Jennifer M. Baker Nicole A. Vander Schaaf Anna M. G. Cunningham Anna C. Hang Chelsea L. Reeves Emily R. Huffman Carli J. Riester Michael T. Madigan W. Matthew Sattley 2019-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2019.00156 https://doaj.org/article/71d11a18b95b484ca5b21806431b9cd9 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2019.00156/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.00156 https://doaj.org/article/71d11a18b95b484ca5b21806431b9cd9 Frontiers in Microbiology, Vol 10 (2019) Antarctica Lake Fryxell McMurdo Dry Valleys chemoorganotroph psychrotolerant halotolerant Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.3389/fmicb.2019.00156 2022-12-31T04:28:35Z Lake Fryxell, situated in the McMurdo Dry Valleys of Antarctica, is an intriguing aquatic ecosystem because of its perennial ice cover, highly stratified water column, and extreme physicochemical conditions, which collectively restrict lake biodiversity to solely microbial forms. To expand our current understanding of the cultivable biodiversity of Lake Fryxell, water samples were collected from depths of 10 and 17 m, and pure cultures of eight diverse strains of aerobic, chemoorganotrophic bacteria were obtained. Despite having high 16S rRNA gene sequence similarity to mesophilic bacteria inhabiting various temperate environments, all Lake Fryxell isolates were psychrotolerant, with growth occurring at 0°C and optimal growth from 18–24°C for all isolates. Phylogenetic analyses showed the isolates to be members of six taxonomic groups, including the genera Brevundimonas, Arthrobacter, Sphingobium, Leifsonia, and Pseudomonas, as well as the family Microbacteriaceae (one strain could not reliably be assigned to a specific genus based on our analysis). Pseudomonas strain LFY10 stood out as a useful tool for teaching laboratory activities because of its substantial cold adaptation (visible growth is evident in 1–2 days at 4°C), beta-hemolytic activity, and halotolerance to 8.5% (w/v) NaCl. These cold-adapted bacteria likely play a role in carbon mineralization and other nutrient cycling in Lake Fryxell, and their characterization broadens our understanding of microbial biodiversity in aquatic polar ecosystems. Article in Journal/Newspaper Antarc* Antarctica McMurdo Dry Valleys Directory of Open Access Journals: DOAJ Articles McMurdo Dry Valleys Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Frontiers in Microbiology 10 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Antarctica Lake Fryxell McMurdo Dry Valleys chemoorganotroph psychrotolerant halotolerant Microbiology QR1-502 |
spellingShingle |
Antarctica Lake Fryxell McMurdo Dry Valleys chemoorganotroph psychrotolerant halotolerant Microbiology QR1-502 Jennifer M. Baker Nicole A. Vander Schaaf Anna M. G. Cunningham Anna C. Hang Chelsea L. Reeves Emily R. Huffman Carli J. Riester Michael T. Madigan W. Matthew Sattley Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs |
topic_facet |
Antarctica Lake Fryxell McMurdo Dry Valleys chemoorganotroph psychrotolerant halotolerant Microbiology QR1-502 |
description |
Lake Fryxell, situated in the McMurdo Dry Valleys of Antarctica, is an intriguing aquatic ecosystem because of its perennial ice cover, highly stratified water column, and extreme physicochemical conditions, which collectively restrict lake biodiversity to solely microbial forms. To expand our current understanding of the cultivable biodiversity of Lake Fryxell, water samples were collected from depths of 10 and 17 m, and pure cultures of eight diverse strains of aerobic, chemoorganotrophic bacteria were obtained. Despite having high 16S rRNA gene sequence similarity to mesophilic bacteria inhabiting various temperate environments, all Lake Fryxell isolates were psychrotolerant, with growth occurring at 0°C and optimal growth from 18–24°C for all isolates. Phylogenetic analyses showed the isolates to be members of six taxonomic groups, including the genera Brevundimonas, Arthrobacter, Sphingobium, Leifsonia, and Pseudomonas, as well as the family Microbacteriaceae (one strain could not reliably be assigned to a specific genus based on our analysis). Pseudomonas strain LFY10 stood out as a useful tool for teaching laboratory activities because of its substantial cold adaptation (visible growth is evident in 1–2 days at 4°C), beta-hemolytic activity, and halotolerance to 8.5% (w/v) NaCl. These cold-adapted bacteria likely play a role in carbon mineralization and other nutrient cycling in Lake Fryxell, and their characterization broadens our understanding of microbial biodiversity in aquatic polar ecosystems. |
format |
Article in Journal/Newspaper |
author |
Jennifer M. Baker Nicole A. Vander Schaaf Anna M. G. Cunningham Anna C. Hang Chelsea L. Reeves Emily R. Huffman Carli J. Riester Michael T. Madigan W. Matthew Sattley |
author_facet |
Jennifer M. Baker Nicole A. Vander Schaaf Anna M. G. Cunningham Anna C. Hang Chelsea L. Reeves Emily R. Huffman Carli J. Riester Michael T. Madigan W. Matthew Sattley |
author_sort |
Jennifer M. Baker |
title |
Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs |
title_short |
Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs |
title_full |
Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs |
title_fullStr |
Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs |
title_full_unstemmed |
Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs |
title_sort |
chemoorganotrophic bacteria from lake fryxell, antarctica, including pseudomonas strain lfy10, a cold-adapted, halotolerant bacterium useful in teaching labs |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.00156 https://doaj.org/article/71d11a18b95b484ca5b21806431b9cd9 |
long_lat |
ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
McMurdo Dry Valleys Fryxell Lake Fryxell |
geographic_facet |
McMurdo Dry Valleys Fryxell Lake Fryxell |
genre |
Antarc* Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctica McMurdo Dry Valleys |
op_source |
Frontiers in Microbiology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2019.00156/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.00156 https://doaj.org/article/71d11a18b95b484ca5b21806431b9cd9 |
op_doi |
https://doi.org/10.3389/fmicb.2019.00156 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766027217343610880 |