Global gene expression responses of Atlantic salmon skin to Moritella viscosa
Abstract Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin...
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ftdoajarticles:oai:doaj.org/article:719e4b8d08f04c52a9d0531b8124dac3 2023-05-15T15:28:31+02:00 Global gene expression responses of Atlantic salmon skin to Moritella viscosa Khalil Eslamloo Surendra Kumar Xi Xue Kathleen S. Parrish Sara L. Purcell Mark D. Fast Matthew L. Rise 2022-03-01T00:00:00Z https://doi.org/10.1038/s41598-022-08341-7 https://doaj.org/article/719e4b8d08f04c52a9d0531b8124dac3 EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-08341-7 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-08341-7 2045-2322 https://doaj.org/article/719e4b8d08f04c52a9d0531b8124dac3 Scientific Reports, Vol 12, Iss 1, Pp 1-16 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-08341-7 2022-12-31T04:01:23Z Abstract Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the lesion (Mv-At) and away from the lesion (Mv-Aw) sites. M. viscosa infection was confirmed through RNA-based qPCR assays. RNA-Seq identified 5212 and 2911 transcripts differentially expressed in the Mv-At compared to no-infection control and Mv-Aw groups, respectively. Also, there were 563 differentially expressed transcripts when comparing the Mv-Aw to control samples. Our results suggest that M. viscosa caused massive and strong, but largely infection site-focused, transcriptome dysregulations in Atlantic salmon skin, and its effects beyond the skin lesion site were comparably subtle. The M. viscosa-induced transcripts of Atlantic salmon were mainly involved in innate and adaptive immune response-related pathways, whereas the suppressed transcripts by this pathogen were largely connected to developmental and cellular processes. As validated by qPCR, M. viscosa dysregulated transcripts encoding receptors, signal transducers, transcription factors and immune effectors playing roles in TLR- and IFN-dependent pathways as well as immunoregulation, antigen presentation and T-cell development. This study broadened the current understanding of molecular pathways underlying M. viscosa-triggered responses of Atlantic salmon, and identified biomarkers that may assist to diagnose and combat this pathogen. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Scientific Reports 12 1 |
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Medicine R Science Q |
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Medicine R Science Q Khalil Eslamloo Surendra Kumar Xi Xue Kathleen S. Parrish Sara L. Purcell Mark D. Fast Matthew L. Rise Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
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Medicine R Science Q |
description |
Abstract Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the lesion (Mv-At) and away from the lesion (Mv-Aw) sites. M. viscosa infection was confirmed through RNA-based qPCR assays. RNA-Seq identified 5212 and 2911 transcripts differentially expressed in the Mv-At compared to no-infection control and Mv-Aw groups, respectively. Also, there were 563 differentially expressed transcripts when comparing the Mv-Aw to control samples. Our results suggest that M. viscosa caused massive and strong, but largely infection site-focused, transcriptome dysregulations in Atlantic salmon skin, and its effects beyond the skin lesion site were comparably subtle. The M. viscosa-induced transcripts of Atlantic salmon were mainly involved in innate and adaptive immune response-related pathways, whereas the suppressed transcripts by this pathogen were largely connected to developmental and cellular processes. As validated by qPCR, M. viscosa dysregulated transcripts encoding receptors, signal transducers, transcription factors and immune effectors playing roles in TLR- and IFN-dependent pathways as well as immunoregulation, antigen presentation and T-cell development. This study broadened the current understanding of molecular pathways underlying M. viscosa-triggered responses of Atlantic salmon, and identified biomarkers that may assist to diagnose and combat this pathogen. |
format |
Article in Journal/Newspaper |
author |
Khalil Eslamloo Surendra Kumar Xi Xue Kathleen S. Parrish Sara L. Purcell Mark D. Fast Matthew L. Rise |
author_facet |
Khalil Eslamloo Surendra Kumar Xi Xue Kathleen S. Parrish Sara L. Purcell Mark D. Fast Matthew L. Rise |
author_sort |
Khalil Eslamloo |
title |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_short |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_full |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_fullStr |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_full_unstemmed |
Global gene expression responses of Atlantic salmon skin to Moritella viscosa |
title_sort |
global gene expression responses of atlantic salmon skin to moritella viscosa |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-08341-7 https://doaj.org/article/719e4b8d08f04c52a9d0531b8124dac3 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-16 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-08341-7 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-08341-7 2045-2322 https://doaj.org/article/719e4b8d08f04c52a9d0531b8124dac3 |
op_doi |
https://doi.org/10.1038/s41598-022-08341-7 |
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Scientific Reports |
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12 |
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1 |
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1766358877529440256 |