CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)
Ostreid herpesvirus-1 microvariant (OsHV-1 μVar) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and f...
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ftdoajarticles:oai:doaj.org/article:718a0dcd82714c2190811b2bfedfa9ce 2023-05-15T15:57:50+02:00 CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) Xueying Tang Baoyu Huang Siheng Lin Wei Wang Guofan Zhang Li Li 2020-07-01T00:00:00Z https://doi.org/10.3389/fimmu.2020.01247 https://doaj.org/article/718a0dcd82714c2190811b2bfedfa9ce EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2020.01247/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.01247 https://doaj.org/article/718a0dcd82714c2190811b2bfedfa9ce Frontiers in Immunology, Vol 11 (2020) innate immunity OsHV-1 μVar Crassostrea gigas TLR MyD88 Immunologic diseases. Allergy RC581-607 article 2020 ftdoajarticles https://doi.org/10.3389/fimmu.2020.01247 2022-12-31T04:05:13Z Ostreid herpesvirus-1 microvariant (OsHV-1 μVar) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and functional divergence of innate immune genes in the oyster have been studied, but the contribution of molecular mechanisms underlying innate immunity remains to be defined. Here, we identified the interacting proteins associated with Crassostrea gigas Toll-like receptors (CgTLR) using a yeast two-hybrid (Y2H) screening system. A total of eight proteins were identified that could interact with CgTLR. Three of these appeared at least four times in the screening and were related to MyD88. Two genes encoding these MyD88-like proteins, CgMyD88-1 and CgMyD88-2, possessed typical death and TIR domains. The third gene encoding an MyD88-like protein possessed only a TIR domain, and we named it CgMyD88s. CgMyD88s interacted only with CgTLR, but not CgMyD88-1 or CgMyD88-2. Both CgMyD88-1 and CgMyD88-2 mRNAs were upregulated after OsHV-1 μVar infection, whereas the expression of CgMyD88s decreased. When overexpressed in HEK293T cells, CgMyD88-1 and CgMyD88-2 activated an NF-κB reporter, whereas CgMyD88s impaired activation induced by CgMyD88-1 or CgMyD88-2. Intriguingly, the silencing of CgMyD88s using double-stranded RNA (dsRNA)-mediated RNA interference increased the expression of CgMyD88-1 and CgMyD88-2. Taken together, our results revealed that CgMyD88-1, CgMyD88-2, and CgMyD88s may all participate in the TLR-mediated innate immune pathway and that CgMyD88s served as a plug to avoid oysters from excessive inflammatory response during OsHV-1 μVar infections. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
innate immunity OsHV-1 μVar Crassostrea gigas TLR MyD88 Immunologic diseases. Allergy RC581-607 |
spellingShingle |
innate immunity OsHV-1 μVar Crassostrea gigas TLR MyD88 Immunologic diseases. Allergy RC581-607 Xueying Tang Baoyu Huang Siheng Lin Wei Wang Guofan Zhang Li Li CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
topic_facet |
innate immunity OsHV-1 μVar Crassostrea gigas TLR MyD88 Immunologic diseases. Allergy RC581-607 |
description |
Ostreid herpesvirus-1 microvariant (OsHV-1 μVar) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and functional divergence of innate immune genes in the oyster have been studied, but the contribution of molecular mechanisms underlying innate immunity remains to be defined. Here, we identified the interacting proteins associated with Crassostrea gigas Toll-like receptors (CgTLR) using a yeast two-hybrid (Y2H) screening system. A total of eight proteins were identified that could interact with CgTLR. Three of these appeared at least four times in the screening and were related to MyD88. Two genes encoding these MyD88-like proteins, CgMyD88-1 and CgMyD88-2, possessed typical death and TIR domains. The third gene encoding an MyD88-like protein possessed only a TIR domain, and we named it CgMyD88s. CgMyD88s interacted only with CgTLR, but not CgMyD88-1 or CgMyD88-2. Both CgMyD88-1 and CgMyD88-2 mRNAs were upregulated after OsHV-1 μVar infection, whereas the expression of CgMyD88s decreased. When overexpressed in HEK293T cells, CgMyD88-1 and CgMyD88-2 activated an NF-κB reporter, whereas CgMyD88s impaired activation induced by CgMyD88-1 or CgMyD88-2. Intriguingly, the silencing of CgMyD88s using double-stranded RNA (dsRNA)-mediated RNA interference increased the expression of CgMyD88-1 and CgMyD88-2. Taken together, our results revealed that CgMyD88-1, CgMyD88-2, and CgMyD88s may all participate in the TLR-mediated innate immune pathway and that CgMyD88s served as a plug to avoid oysters from excessive inflammatory response during OsHV-1 μVar infections. |
format |
Article in Journal/Newspaper |
author |
Xueying Tang Baoyu Huang Siheng Lin Wei Wang Guofan Zhang Li Li |
author_facet |
Xueying Tang Baoyu Huang Siheng Lin Wei Wang Guofan Zhang Li Li |
author_sort |
Xueying Tang |
title |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_short |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_full |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_fullStr |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_full_unstemmed |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_sort |
cgmyd88s serves as an innate immune system plug during ostreid herpesvirus 1 infection in the pacific oyster (crassostrea gigas) |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.01247 https://doaj.org/article/718a0dcd82714c2190811b2bfedfa9ce |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2020.01247/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.01247 https://doaj.org/article/718a0dcd82714c2190811b2bfedfa9ce |
op_doi |
https://doi.org/10.3389/fimmu.2020.01247 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
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1766393530248331264 |